Progression of experimental chronic Aleutian mink disease virus infection

BACKGROUND: Aleutian mink disease virus (AMDV) is found world-wide and has a major impact on mink health and welfare by decreasing reproduction and fur quality. In the majority of mink, the infection is subclinical and the diagnosis must be confirmed by serology or polymerase chain reaction (PCR). Increased knowledge based on a systematically description of clinical signs, pathology and histopathology might be a tool to reduce the risk of infection from subclinically infected mink to AMDV free herds. The aim of this study was to give a histopathological description of the progression of a chronic experimental infection with a currently circulating Danish strain of AMDV, Saeby/DEN/799.1/05. These results were compared with the pathogenesis of previously published AMDV stains. RESULTS: This experimental AMDV infection resulted in only decreased appetite and soft or discolored feces, primarily within the first 8 weeks after AMDV inoculation. Gross pathology revealed few and inconsistent findings mainly associated with the liver, spleen and kidneys. The majority of the AMDV inoculated wild type mink (n = 41) developed various histopathological changes consistent with AMDV infection in one or more organs: infiltrations of mononuclear cells in liver, kidney and brain, reduced density of lymphocytes and increased numbers of plasma cells in lymph nodes and spleen. Natural infection, as occurred in the sentinel sapphire mink (four of six mink), progressed similar to the experimentally inoculated mink. CONCLUSIONS: Experimental AMDV inoculation mainly resulted in subclinical infection with unspecific clinical signs and gross pathology, and more consistent histopathology appearing at any time after AMDV inoculation during the 24 weeks of observation. Thus, the observed histopathology substantiates AMDV infection and no correlation to time of inoculation was found. This confirms that diagnosing AMDV infection requires serology and/or PCR and the Saeby/DEN/799.1/05 AMDV strain results in histopathology consistent with other AMDV strains

Development of body mass and sexual size dimorphism in Danish red foxes (Vulpes vulpes)

<span class="fontstyle0">In this study, we examine the development of body mass and sexual size dimorphism (SSD) in 178 juvenile wild Danish red </span><span class="fontstyle0">foxes </span><span class="fontstyle0">from 99 litters </span><span class="fontstyle0">using </span><span class="fontstyle0">piecewise analyses of regression lines for age </span><span class="fontstyle2">versus</span><span class="fontstyle0"> weight</span><span class="fontstyle0">. When fox cubs are younger than 100 days, only slight (SSD=1.7%) and no significant difference</span><span class="fontstyle0"> (t-test: t=1.2, p=0.24) </span><span class="fontstyle0">was found in the mean weight of </span><span class="fontstyle0">males (2.03± kg) and females (1.93± kg), and</span><span class="fontstyle0"> no significant difference was found in the slope of regression lines </span><span class="fontstyle0">for </span><span class="fontstyle0">males and females </span><span class="fontstyle0">(F=0.97E-5, p = 0.99). In the growth period between 100 days of age and mating around 275 days of age, the regression line in males steepens more than that of females (difference in slopes, F=5.9, p&lt;0.02) and the difference in mean weight of the sexes become highly significant (SSD=7.4%, difference in mean t=4.6, p=2.2E-5). After mating the growth curve levels off i.e. the slope of the regression lines for age </span><span class="fontstyle2">versus</span><span class="fontstyle0"> weight is not significantly different from zero. Yearly variation was revealed in the growth rate of juvenile foxes (difference in slope for males; F=3.9, p&lt;0.01 and females; F=8.6, p&lt;0.001). Conclusion: SSD in red foxes mainly develop </span><span class="fontstyle0">as a result of a faster grow rate in males </span><span class="fontstyle0">between indepency and maturity. Ontogony of red foxes may genetically be disposed to prevent males outcompeting females in the early stages of life (&lt;100 days), when cubs are still fed by adults and the increase in SSD before mating, may be an adaption to selective forces benefitting larger males. </span><span class="fontstyle0">The growth rate of juvenile foxes of both sexes is influenced by environmental variation in different years.</span> <br /

Baylisascaris procyonis in wild raccoons (Procyon lotor) in Denmark

AbstractThe nematode Baylisascaris procyonis, which may cause severe clinical disease in humans and animals, is emerging in Europe after its introduction with raccoons (Procyon lotor) from North America. B. procyonis has a broad spectrum of paratenic hosts, including rodents, birds, wild carnivores and primates, which are severely affected by the migrating larval stages of the parasite. We report here the recovery of B. procyonis from two out of 18 examined wild raccoons in Denmark. The parasites were identified based on morphology and their identity was confirmed by partial sequencing of the 18S rRNA gene. Follow-up telephone interviews of staffs in nine zoos housing captive raccoons and veterinarians supervising these zoos showed that knowledge of B. procyonis and its zoonotic potential were sparse. Eggs of B. procyonis were detected in two raccoons kept in one of three zoos that submitted fecal samples following the telephone interviews. Continuous monitoring and increased awareness are needed to reduce further spread of the parasite and to limit the public health risks associated with baylisascariasis