Hemolytic activity of C-5.

Hemolysis of horse red blood cells was done in presence of Triton-X (control) and different concentrations of C-5.</p

Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) of eugenol tosylate congeners (C1-C6) against different <i>C</i>. <i>auris</i> isolates.

Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) of eugenol tosylate congeners (C1-C6) against different C. auris isolates.</p

Design and synthesis of Co(II) and Cu(II) complexes of a dendrimeric chelate: promising anticandidal potential of chelotherapeutic agents

<div><p>In response to an increasing demand for effective anticandidal agents, a new water-soluble dendrimeric ligand (L) was synthesized by Michael addition of ethylenediamine to methyl methacrylate. The prepared ligand was complexed with Cu(II) and Co(II) ions. Both the ligand and its complexes were characterized by elemental analysis and spectroscopic studies (FT-IR, UV–vis, ¹H NMR, and ESI-MS). Square-planar and square-pyramidal geometries were proposed for Cu(II) and Co(II) on the basis of UV–vis spectroscopic data and molar conductance measurements. The ligand and its Cu(II) and Co(II) complexes were screened on <i>Candida albicans</i> ATCC 90028 by determining MICs (minimal inhibitory concentrations) and inhibition in solid media (disk diffusion assay). Hemolysis assays on human RBCs indicated that the toxicity of the copper complex was lower as compared to fluconazole. These results taken with limited toxicity make them eligible for further development as antifungals.</p></div

TUNNEL assay representing apoptosis in <i>C</i>. <i>auris</i>.

The confocal microscopy of C. auris 6057 cells treated with different concentrations (½MIC, MIC, and 2MIC) of C-5 compound. Hoechst 33342 dye (blue fluorescence) showing live cells, and Alexa Fluor 488 dye (green fluorescence) represent apoptotic cells.</p

Zone of diameters of LF, 21LF, 38LF and 45LF against <i>Candida albicans</i>.

<p>Zone of diameters of LF, 21LF, 38LF and 45LF against <i>Candida albicans</i>.</p

Superimposition of the backbone chains of iron-saturated LFs from various species.

<p>The figure shows that the overall chain folds in a similar fashion in all the species.</p

LC₉₀ values of LF, 21LF, 38LF and 45LF against <i>Candida albicans</i>.

<p>All the data were expressed as mean values ± standard deviations; <i>P</i><0.05, student's <i>t-</i> test.</p

Elution profile during the purification of the hydrolyzate of bovine LF after trypsin digestion.

<p>Elution was performed using ion exchange chromatography by CM-Sephadex C-50 in 0.05 M Tris-HCl, pH 8.0. Peak 1 indicates the unbound fraction while Peak 2 is eluted in the bound fraction.</p

Zone of inhibitions against <i>C. albicans</i> ATCC 90028.

<p>A corresponds to Fluconazole (positive control), B corresponds to 21LF, C corresponds to 38LF, D corresponds to 45LF, E corresponds to LF and F corresponds to blank.</p

Antifungal activity of LF and its three tryptic fragments.

<p>The antifungal activity of (A) LF (•), (B) 21LF (♦), (C) 38LF (▾) and (D) 45LF (▪) is plotted against <i>C. albicans</i> ATCC 90028. All the data were expressed as mean values ± standard deviations; <i>P</i><0.05, student's <i>t-</i> test</p