Generation of a ribosomal DNA probe by PCR and its use in identification of fungi within the Gaeumannomyces-Phialophora complex

The polymerase chain reaction (PCR) was used to amplify a ribosomal DNA fragment from Gaewnannomyces graminis. This fragment was labelled and tested for its usefulness as a probe in restriction fragment length polymorphism (RFLP) studies of fungi within the Gaeumannomyces-Phialophora complex. When the probe was hybridized to EcoRI digests of DNA from these fungi, there were consistent band pattern differences between the three varieties of G. graminis (tritici, avenae and graminis). This method of probe production, which is more rapid than many others currently used, has considerable potential for use in the identification of these organisms, and may also be applicable to other groups of fungi.Peer reviewe

AGENDA-SETTING BY STATE LEGISLATIVE BLACK CAUCUSES: POLICY PRIORITIES AND FACTORS OF SUCCESS

As their representation in legislative chambers has increased, black state legislators have organized into caucuses to maximize their policy influence. These caucuses act as policy entrepreneurs in initiating and advancing minority policy goals. The North Carolina Legislative Black Caucus is il- lustrative of an emerging force in half the states in moving minority policy issues from the systemic to the formal legislative agenda. Using the 1987 North Carolina Legislative Black Caucus as the research setting, supple- mented with selective data on other state caucuses, this article offers insights into the types of policy initiatives state legislative black caucuses advance. Also, situational and political factors that may foster successful agenda-setting are explored. Copyright 1989 by The Policy Studies Organization.