Morphological, Biochemical and Proline-Related Genes Analyses in Resistant and Susceptible Wheat Cultivars in Iraq

A high salinity level negatively affects the morpho-physiological parameters of plants. As a result of salinity, these effects are considered crucial signs of plant damage.  In this study, ten Iraqi wheat cultivars were examined against two salinity levels (3 and 15 ds m-1) and some morphological, biochemical properties were measured. Also proline-related genes were analyzed using Ilumina RNA sequences and bioinformatics analyses. All cultivars demonstrated a decrease in the studied parameters with an increase saliness. 'Dijlah' cultivar showed best performance salinity stress, while 'Ibaa 99' was sensitive based on morphological and biochemical parameters. The competition was in favor of the sodium ion at the expense of the potassium ion in high salinity conditions. Proline accumulation in wheat blade leaves was about 2.5 times higher at the peak salt level. The transcriptomic analysis was done and the transcripts per million (TPM) values were estimated for some proline genes. The genes of probable proline transporter 2, proline     dehydrogenase 2, and GSK-like kinase 1A obtained the higher TPM values in cultivar 'Dijlah' cultivar than in 'Ibaa 99' cultivar. It can be concluded that 'Dijlah' cultivar is a salt tolerant cultivar as compare with the susceptible 'Ibaa 99' cultivar, and their proline accumulation was increased with salinity stress and was related with TPM values.  Morphological, biochemical and TPM values would offer a good combined- criteria for recognition the tolerant genotype

Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers

DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.7

International Journal of Multidisciplinary and Current Research Salinity effect on wheat Triticum aestivum L. callus growth and development

Abstract This research was conducted to study the effect of salinity on callus induction and development of two local wheat cultivars Tamoz2 and Iraq. Four different methods were used to sterilize the mature seeds. Sterilizing the seeds before and after soaking them in water with absolute Ethanol for 1 min. followed by 6% NaOCl for 10 min. showed 0% contamination and didn't affect embryos viability. However, there was no significant difference in the percentage of contamination between this method and the method in which 70% Ethanol for 1 min. followed by 4.2% NaOCl for 10 min. before and after soaking them in water. Calli were induced from mature embryos and subjected to sodium chloride o

Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers

DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.7