Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation.

Agrin is thought to be the nerve-derived factor that initiates acetylcholine receptor (AChR) clustering at the developing neuromuscularjunction. We have investigated the signaling pathway in mouse C2 myotubes and report that agrin induces a rapid but transient tyrosine phosphorylation of the AChR beta subunit. As the beta-subunit tyrosine phosphorylation occurs before the formation of AChR clusters, it may serve as a precursor step in the clustering mechanism. Consistent with this, we observed that tyrosine phosphorylation of the beta subunit correlated precisely with the presence or absence of clustering under several experimental conditions. Moreover, two tyrosine kinase inhibitors, herbimycin and staurosporine, that blocked beta-subunit phosphorylation also blocked agrin-induced clustering. Surprisingly, the inhibitors also dispersed preformed AChR clusters, suggesting that the tyrosine phosphorylation of other proteins may be required for the maintenance of receptor clusters. These findings indicate that in mammalian muscle, agrin-induced AChR clustering occurs through a mechanism that requires tyrosine phosphorylation and may involve tyrosine phosphorylation of the AChR itself

A flexible one-pot route to metal/metal oxide nanocomposites

We report a one-pot route to Au/CeO2 nanocomposites. A readily-available biopolymer, sodium alginate, is exploited for controlled formation and stabilisation of gold nanoparticles followed by in situ growth of a sponge-like network of CeO2 nanoparticles. The flexible nature of this method as a general route to mixed metal/metal oxide nanocomposites is also demonstrated

Late Time Neutrino Masses, the LSND Experiment and the Cosmic Microwave Background

Models with low-scale breaking of global symmetries in the neutrino sector provide an alternative to the seesaw mechanism for understanding why neutrinos are light. Such models can easily incorporate light sterile neutrinos required by the LSND experiment. Furthermore, the constraints on the sterile neutrino properties from nucleosynthesis and large scale structure can be removed due to the non-conventional cosmological evolution of neutrino masses and densities. We present explicit, fully realistic supersymmetric models, and discuss the characteristic signatures predicted in the angular distributions of the cosmic microwave background.Comment: 4 pages, revtex

The Weak Mixing Angle From TeV Scale Quark-Lepton Unification

Unified theories based on an extended left-right symmetric group, $SU(4) \times SU(2)^4$, are constructed in five dimensions. The compactification scale is assumed to be only a loop factor above the weak scale, so that the weak mixing angle is predicted to be close to its tree level value of 0.239. Boundary conditions in the 5th dimension break $SU(4) \to SU(3) \times U(1)_{B-L}$, removing powerful constraints from $K_L \to \mu e$ while allowing a reliable calculation of the leading logarithm corrections to $\sin^2 \theta$. The compactification scale is expected in the 1--5 TeV region, depending on how $SU(2)^4$ is broken. Two illustrative models are presented, and the experimental signal of the $Z'$ gauge boson is discussed.Comment: 15 page

The principle of equivalence and projective structure in space-times

This paper discusses the extent to which one can determine the space-time metric from a knowledge of a certain subset of the (unparametrised) geodesics of its Levi-Civita connection, that is, from the experimental evidence of the equivalence principle. It is shown that, if the space-time concerned is known to be vacuum, then the Levi-Civita connection is uniquely determined and its associated metric is uniquely determined up to a choice of units of measurement, by the specification of these geodesics. It is further demonstrated that if two space-times share the same unparametrised geodesics and only one is assumed vacuum then their Levi-Civita connections are again equal (and so the other metric is also a vacuum metric) and the first result above is recovered.Comment: 23 pages, submitted to Classical and Quantum Gravit

On the Theory of Killing Orbits in Space-Time

This paper gives a theoretical discussion of the orbits and isotropies which arise in a space-time which admits a Lie algebra of Killing vector fields. The submanifold structure of the orbits is explored together with their induced Killing vector structure. A general decomposition of a space-time in terms of the nature and dimension of its orbits is given and the concept of stability and instability for orbits introduced. A general relation is shown linking the dimensions of the Killing algebra, the orbits and the isotropies. The well-behaved nature of "stable" orbits and the possible miss-behaviour of the "unstable" ones is pointed out and, in particular, the fact that independent Killing vector fields in space-time may not induce independent such vector fields on unstable orbits. Several examples are presented to exhibit these features. Finally, an appendix is given which revisits and attempts to clarify the well-known theorem of Fubini on the dimension of Killing orbits.Comment: Latex, 19 pages, no figur

Severe Fermi Surface Reconstruction at a Metamagnetic-Transition in Ca2−x_{2-x}Srx_xRuO4_4 (for 0.2≤x≤0.50.2 \leq x \leq 0.5)

We report an electrical transport study in Ca$_{2-x}$Sr$_{x}$RuO$_4$ single crystals at high magnetic fields ($B$). For $x =0.2$, the Hall constant $R_{xy}$ decreases sharply at an anisotropic metamagnetic (MM) transition reaching its value for Sr$_2$RuO$_4$ at high fields. A sharp decrease in the $A$ coefficient of the resistivity $T^2$-term and a change in the structure of the angular magnetoresistance oscillations (AMRO) for $B$ rotating in the planes, confirms the reconstruction of the Fermi surface (FS). Our observations and LDA calculations indicate a strong dependence of the FS on the Ca concentration and suggest the coexistence of itinerant and localized electronic states in single layered ruthenates.Comment: 5 pages, 4 fig

High Field de Haas - van Alphen Studies of the Fermi Surfaces of LaMIn5_{5} (M = Co, Rh, Ir)

We report measurements of the de Haas - van Alphen effect on a series of compounds, LaMIn$_{5}$ (M = Co, Rh, Ir). The results show that each of the Co and Ir Fermi surfaces (FSs) exhibit some portions that are two dimensional and some portions that are three dimensional. The most two dimensional character is exhibited in LaCoIn$_{5}$, less two dimensional behavior is seen in LaIrIn$_{5}$, no part of Fermi surface of LaRhIn$_{5}$ is found to have a two dimensional character. Thus the two dimensionality of portions of the FSs is largely determined by the d character of the energy bands while all of the effective masses remain $\leq$ 1.2. This fact has implications for the causes of the heavy fermion nature of superconductivity and magnetism in the Ce-based compounds having the similar composition and structure. All of the measurements were performed at the National High Magnetic Field Laboratory using either cantilever magnetometry or field modulation methods.Comment: 10 pages, 4 figure