Research on Microstructure and Properties of Medium Carbon Steel Parts Manufactured by HDMR Technology

A new study on manufacturing medium carbon steel parts by HDMR (Hybrid Deposition and Micro Rolling) technology was carried out, and the microstructures and mechanical properties obtained by HDMR process and freeform arc deposition process respectively were tested and compared in this paper. The experiment results show that: compared with the freeform arc deposition process, the grain size number obtained by HDMR process increased from 3.0 to 9.0；the tensile strength and yield strength were increased by 37.1%, 68.6%，in contrast to the investment casting, increased by 65.4% and 107.7%; compared with the forging, the tensile strength and yield strength were increased by 12.9% and 31.4% respectively. Finally, a medium carbon 45 steel aeronautical part difficult to overlay was manufactured successfully by HDMR technology, thus a new efficient way for additive manufacturing of hard-shaping metal parts at high-quality with low-cost was provided.Mechanical Engineerin

Constraint of Water Resources and Countermeasures for Local Sustainable Development of Xi'an

With tremendous pressure of population on natural resources and environment, sustainable utilization of water resources is one of the key problems that is to be solved urgently with the development of XiŽan, which is one of important cities in west China. Based on sufficient data such as hydrometrical data and yearbook statistical data, an analysis of current situation of water resources in XiŽan city has been done. Furthermore, the paper examines various factors that contribute to the shortage of water resources in XiŽan city, those are: lack of water resources, excessively rapid urban development, aggravated situation of waste water and pollution. Finally, countermeasures of the sustainable utilization of its water resources are put forwards.vokMyynti MTT tietopalvelu

A Fourier Transform-Based Calculation Method of Wilting Index for Soybean Canopy Using Multispectral Image

The growth process of soybean plants needs a lot of water. The rapid detection of canopy wilting of soybean under drought stress is of great significance for soybean variety breeding, cultivation regulation and fine management. Aiming at the problems of cumbersome and time-consuming when the traditional chemical technology was used to determine soybean wilting index, a calculation method of wilting index for soybean canopy was proposed in this study based on multispectral images’ Fourier transform. Suinong 26, a northeast soybean variety, was taken as the object. First, four kinds of soybean multispectral images of green, red, red-edge and near-infrared channels were acquired by a Sequoia multispectral camera. Second, based on the multispectral reflection image preprocessed by median filter and mean filter, the target area of a multispectral image of the soybean canopy was extracted by the iterative threshold method and affine transformation algorithm, and the effective segmentation rate was 97.02%. In addition, Fourier transform was used to analyze the spectrum characteristics of the soybean canopy’s multispectral image. When the spectrum radius of each channel was 50, the energy reached more than 98% and was concentrated in the low-frequency region of the spectrum center. Finally, according to the difference between the low-frequency DC component and the proportion of total energy in the spectral radius of the multispectral images under normal and drought treatment, a calculation model of the soybean wilting index was constructed based on the energy spectrum of Fourier transform. The results showed that the difference of the wilting index between normal and drought treatment for the four channels (green, near-infrared, red and red-edge) was 2.38, 3.11, 3.56 and 4.11, respectively. The effectiveness of the wilting index was verified and analyzed by using the average leaf inclination angle. The determination coefficient R2 of the four channels between the calculated wilting index and the average leaf inclination angle was more than 0.85. This calculation method can provide a quantitative basis and technical support for the scientific regulation of ecological and morphological phenotypic traits of soybean plants under drought stress

Use of a local worker survey as a source of neighborhood information

Researchers increasingly recognize the potential influence of the neighborhood environment on individual health and social behavior. To examine these influences, it is important to use varying measures and sources of neighborhood characteristics. Though neighborhood residents are often surveyed, the perceptions of neighborhood workers have been largely ignored. The current study documents procedures and findings from two longitudinal studies in which workers in 60 neighborhoods were surveyed about neighborhood social cohesion and social control (collective efficacy), and neighborhood problems. Results indicated that workers within neighborhoods were more homogeneous in their views of neighborhood collective efficacy and neighborhood problems than were workers across neighborhoods. In addition, not only were workers' perceptions of their neighborhoods similar to the perceptions of neighborhood residents but they also provided unique information. Overall, this study demonstrates the viability and usefulness of local workers as an additional source of neighborhood information. Keywords: neighborhoods; collective efficacy; measures; multilevel modeling R esearchers increasingly recognize the need for linking micro-and macrolevel dimensions of social behavior. Neighborhood demographic Environment and Behavior Volume XX Number X Month XXXX xx-x

CXCL13 shapes immunoactive tumor microenvironment and enhances the efficacy of PD-1 checkpoint blockade in high-grade serous ovarian cancer

Background Most patients with high-grade serous ovarian cancer (HGSC) lack an effective response to immune checkpoint blockade, highlighting the need for more knowledge about what is required for successful treatment. As follicular cytotoxic CXCR5+CD8+ T cells are maintained by reinvigoration by immune checkpoint blockade in tumors, we attempted to reveal the relationship between CXCR5+CD8+ T cells and the tumor microenvironment to predict immunotherapy responses in HGSC.Methods 264 patients with HGSC from two cohorts and 340 HGSC cases from The Cancer Genome Atlas cohort were enrolled. Ex vivo and in vivo studies were conducted with human HGSC tumors and murine tumor models. The spatial correlation between CXC-chemokine ligand 13 (CXCL13), CXCR5, CD8, and CD20 was evaluated by immunohistochemistry and immunofluorescence. Survival was compared between different subsets of patients using Kaplan-Meier analysis. The therapeutic effect of CXCL13 and programmed cell death-1 (PD-1) blockade was validated using human HGSC tumors and murine models.Results High CXCL13 expression was associated with prolonged survival. Tumors with high CXCL13 expression exhibited increased infiltration of activated and CXCR5-expressing CD8+ T cells. Incubation with CXCL13 facilitated expansion and activation of CXCR5+CD8+ T cells ex vivo. CXCR5+CD8+ T cells appeared in closer proximity to CXCL13 in tumors and chemotaxis towards CXCL13 in vitro. The combination of CXCL13, CXCR5, and CD8+ T cells was an independent predictor for survival. In addition, CXCL13 was associated with clusters of CD20+ B cells. CD20+ B cells predicted better patient survival in the presence of CXCL13. Histological evaluation highlighted colocalization of CXCL13 with tertiary lymphoid structures (TLSs). TLSs carried prognostic benefit only in the presence of CXCL13. CXCL13 in combination with anti-PD-1 therapy retarded tumor growth in a CD8+ T-cell-dependent manner, resulting in increased infiltration of cytotoxic CD8+ T cells and CXCR5+CD8+ T cells.Conclusions These data define a critical role of CXCL13 in shaping antitumor microenvironment by facilitating the maintenance of CXCR5+CD8+ T cells in TLSs and support a clinical investigation for a combination of CXCL13 and PD-1 blockade therapy in HGSC

A Fourier Transform-Based Calculation Method of Wilting Index for Soybean Canopy Using Multispectral Image

The growth process of soybean plants needs a lot of water. The rapid detection of canopy wilting of soybean under drought stress is of great significance for soybean variety breeding, cultivation regulation and fine management. Aiming at the problems of cumbersome and time-consuming when the traditional chemical technology was used to determine soybean wilting index, a calculation method of wilting index for soybean canopy was proposed in this study based on multispectral images’ Fourier transform. Suinong 26, a northeast soybean variety, was taken as the object. First, four kinds of soybean multispectral images of green, red, red-edge and near-infrared channels were acquired by a Sequoia multispectral camera. Second, based on the multispectral reflection image preprocessed by median filter and mean filter, the target area of a multispectral image of the soybean canopy was extracted by the iterative threshold method and affine transformation algorithm, and the effective segmentation rate was 97.02%. In addition, Fourier transform was used to analyze the spectrum characteristics of the soybean canopy’s multispectral image. When the spectrum radius of each channel was 50, the energy reached more than 98% and was concentrated in the low-frequency region of the spectrum center. Finally, according to the difference between the low-frequency DC component and the proportion of total energy in the spectral radius of the multispectral images under normal and drought treatment, a calculation model of the soybean wilting index was constructed based on the energy spectrum of Fourier transform. The results showed that the difference of the wilting index between normal and drought treatment for the four channels (green, near-infrared, red and red-edge) was 2.38, 3.11, 3.56 and 4.11, respectively. The effectiveness of the wilting index was verified and analyzed by using the average leaf inclination angle. The determination coefficient R2 of the four channels between the calculated wilting index and the average leaf inclination angle was more than 0.85. This calculation method can provide a quantitative basis and technical support for the scientific regulation of ecological and morphological phenotypic traits of soybean plants under drought stress

C5aR1 blockade reshapes immunosuppressive tumor microenvironment and synergizes with immune checkpoint blockade therapy in high-grade serous ovarian cancer

ABSTRACTHigh-grade serous ovarian cancer (HGSC), with a modest response to immune checkpoint blockade (ICB) targeting PD-1/PD-L1 monotherapy, is densely infiltrated by M2-polarized tumor-associated macrophages (TAMs) and regulatory T (Treg) cells. The complement C5a/C5aR1 axis contributes to the programming of the immunosuppressive phenotype of TAMs in solid tumors and represents a promising immunomodulatory target for treating HGSCs. Here, we aimed to identify the relevance of C5aR1 in prognosis, immune microenvironment, and immunotherapy response in HGSCs. The expression and relationship of C5aR1 with tumor-infiltrating immune cells were assessed by immunohistochemistry and flow cytometry in the training cohort (n = 120) and fresh HGSC tissues (n = 36). Transcriptomic analyses of the xenografts delineated the mechanisms driving the immunomodulatory activity of PMX53, an orally bioavailable C5aR1 inhibitor. Therapeutic relevance was confirmed in ex vivo tumor cultures and The Cancer Genome Atlas (TCGA) datasets. C5aR1 expression independently predicted dismal prognosis and was linked to the immunoevasive subtype of HGSC, characterized by increased infiltration of pro-tumor cells (Treg cells, M2-polarized macrophages, and neutrophils) and impaired CD8+T functions. PMX53 antagonized subcutaneous tumor growth, modulated immunosuppressive mechanisms and synergized with aPD-1 in several tumor types. Single-cell RNA-seq analysis revealed predominant C5aR1 expression in TAMs, with an immunosuppressive-related expression signature in C5aR1+TAMs. Furthermore, the combination of C5aR1 and PD-L1 was associated with specific molecular characteristics and matched clinical response annotations. Therefore, the abundance of C5aR1 could predict an inferior prognosis in HGSCs, and incorporating PD-L1 may serve as a novel predictive biomarker to guide therapeutic options