Helicoid Morphology of Arthrospira platensis NIES-39 Confers Temperature Compensation in the Longitudinal Movement Velocity of Its Trichomes

The velocity of the gliding movement of filamentous cyanobacteria on a solid surface usually has a strong temperature dependency, and the higher the temperature, the faster the speed. Former studies on this phenomenon were conducted using filamentous cyanobacteria with straight morphology. We examined the velocity of the gliding movement of Arthrospira platensis NIES-39 along its longitudinal axis to see if the same was true for this cyanobacterium with helicoid trichomes. Experimental results showed little temperature dependency in the velocity in a wide temperature range in this cyanobacterium. However, when we examined the velocity using mutants with straight trichomes, their velocity was strongly affected by temperature, like other formerly analyzed filamentous cyanobacteria. This result indicates that the helicoid morphology of A. platensis trichomes confers temperature compensation to their migration velocity, enabling them to keep a relatively constant velocity under various temperatures. Migration of wild-type trichomes is considerably suppressed compared to the straight-trichome mutants on solid media. The temperature compensation in the locomotion of this organism appears to be established as part of such a suppression. It was also found that the velocity of this cyanobacterium depended on the trichome length when they were atypically short (<250 µm); the shorter the trichomes, the slower the gliding movement tended to be. This result indicates that the coordinated action of a high number of cells constituting the trichome is required for efficient gliding movement

The RFLP mapping of the calmodulin gene of Neurospora crassa

The map position of the calmodulin gene (cmd) was determined by RFLP (Restriction Fragment Length Polymorphism) mapping in Neurospora crassa. The cmd gene was mapped on chromosome V, between al-3 and inl

Short- and Long-Term Observations of Fracture Permeability in Granite by Flow-Through Tests and Comparative Observation by X-Ray CT

Having a grasp of the variation in the fracture contact area is a kernel in the understanding of the permeability evolution of fractured rocks. However, the number of studies that focus on measuring the long-term variation in the fracture contact area under different conditions is insufficient. In this study, a series of short- and long-term permeability tests under coupled conditions is performed to check the performance of permeability. The results reveal that the permeability measured in the short-term tests shows reversible behavior and a dependence on the applied confining pressures and temperature. In contrast, the permeability in the long-term tests displays irreversible behavior and an irregular change under the constant confining pressure. In order to verify the evolution of permeability, microfocus X-ray computed tomography (CT) is developed to observe the changes in the internal fracture structure under the same conditions as those in long-term permeability tests by assembling a triaxial cell with heating capability. The fracture aperture and the fracture contact-area ratio are calculated by a CT image analysis technique. The image analysis results show that the estimated aperture is seen to decrease with an increase in the confining pressure and to also decrease with time under a constant confining pressure. Moreover, the increase in the fracture contact area under the constant confining pressure observed by X-ray CT is confirmed. This also corresponds to a decrease in permeability in long-term tests. The hydraulic aperture calculated from the permeability tests and that evaluated from the CT observation have a similar decreasing trend. Therefore, the CT observation can better capture the evolution of the internal fracture contact area. These experiments underscore the importance of mechanical compaction and/or mineral dissolution at contacts in determining the rates and the magnitude of permeability evolution within rock fractures

Sheet Dependence on Superconducting Gap in Oxygen-Deficient Iron-based Oxypnictide Superconductors NdFeAs0.85

Photoemission spectroscopy with low-energy tunable photons on oxygen-deficient iron-based oxypnictide superconductors NdFeAsO0.85 (Tc=52K) reveals a distinct photon-energy dependence of the electronic structure near the Fermi level (EF). A clear shift of the leading-edge can be observed in the superconducting states with 9.5 eV photons, while a clear Fermi cutoff with little leading-edge shift can be observed with 6.0 eV photons. The results are indicative of the superconducting gap opening not on the hole-like ones around Gamma (0,0) point but on the electron-like sheets around M(pi,pi) point.Comment: 8 pages, 3 figure