XMM-Newton and Chandra Cross Calibration Using HIFLUGCS Galaxy Clusters: Systematic Temperature Differences and Cosmological Impact

Cosmological constraints from clusters rely on accurate gravitational mass estimates, which strongly depend on cluster gas temperature measurements. Therefore, systematic calibration differences may result in biased, instrument-dependent cosmological constraints. This is of special interest in the light of the tension between the Planck results of the primary temperature anisotropies of the CMB and Sunyaev-Zel'dovich plus X-ray cluster counts analyses. We quantify in detail the systematics and uncertainties of the cross-calibration of the effective area between five X-ray instruments, EPIC-MOS1/MOS2/PN onboard XMM-Newton and ACIS-I/S onboard Chandra, and the influence on temperature measurements. Furthermore, we assess the impact of the cross calibration uncertainties on cosmology. Using the HIFLUGCS sample, consisting of the 64 X-ray brightest galaxy clusters, we constrain the ICM temperatures through spectral fitting in the same, mostly isothermal, regions and compare them. Our work is an extension to a previous one using X-ray clusters by the IACHEC. Performing spectral fitting in the full energy band we find that best-fit temperatures determined with XMM-Newton/EPIC are significantly lower than Chandra/ACIS temperatures. We demonstrate that effects like multitemperature structure and different relative sensitivities of the instruments at certain energy bands cannot explain the observed differences. We conclude that using XMM-Newton/EPIC, instead of Chandra/ACIS to derive full energy band temperature profiles for cluster mass determination results in an 8% shift towards lower OmegaM values and <1% shift towards higher sigma8 values in a cosmological analysis of a complete sample of galaxy clusters. Such a shift is insufficient to significantly alleviate the tension between Planck CMB anisotropies and SZ plus XMM-Newton cosmological constraints.Comment: Accepted by A&A; Python-Script for modification of XMM-Newton/EPIC and Chandra/ACIS effective areas according to the stacked residual ratios: https://wikis.mit.edu/confluence/display/iachec/Data

ROSAT and BeppoSAX evidence of soft X-ray excess emission in the Shapley supercluster: A3571, A3558, A3560 and A3562

Excess soft X-ray emission in clusters of galaxies has so far been detected for sources that lie along lines-of-sight to very low Galactic HI column density (such as Coma, A1795, A2199 and Virgo, N_H 0.9-2.0 10^{20} cm-2). We show that the cluster soft excess emission can be investigated even at higher N_H, which provides an opportunity for investigating soft X-ray emission characteristics among a large number of clusters. The ROSAT PSPC analysis of some members of the Shapley concentration (A3571, A3558, A3560 and A3562, at N_H 4-4.5 10^{20} cm-2) bears evidence for excess emission in the 1/4 keV band. We were able to confirm the finding for the case of A3571 by a pointed SAX observation. Within the current sample the soft X-ray flux is again found to be consistently above the level expected from a hot virialized plasma. The data quality is however insufficient to enable a discrimination between alternative models of the excess low energy flux.Comment: ApJL in press, 5 figure

X-ray total mass estimate for the nearby relaxed cluster A3571

We constrain the total mass distribution in the cluster A3571, combining spatially resolved ASCA temperature data with ROSAT imaging data with the assumption that the cluster is in hydrostatic equilibrium. The total mass within r_500 (1.7/h_50 Mpc) is M_500 = 7.8[+1.4,-2.2] 10^14/ h_50 Msun at 90% confidence, 1.1 times smaller than the isothermal estimate. The Navarro, Frenk & White ``universal profile'' is a good description of the dark matter density distribution in A3571. The gas density profile is shallower than the dark matter profile, scaling as r^{-2.1} at large radii, leading to a monotonically increasing gas mass fraction with radius. Within r_500 the gas mass fraction reaches a value of f_gas = 0.19[+0.06,-0.03] h_50^{-3/2} (90% confidence errors). Assuming that this value of f_gas is a lower limit for the the universal value of the baryon fraction, we estimate the 90% confidence upper limit of the cosmological matter density to be Omega_m < 0.4.Comment: 10 pages, 4 figures, accepted by Ap

Exosome-mediated Transfer of αvβ3 Integrin from Tumorigenic to Nontumorigenic Cells Promotes a Migratory Phenotype.

The αvβ3 integrin is known to be highly upregulated during cancer progression and promotes a migratory and metastatic phenotype in many types of tumors. We hypothesized that the αvβ3 integrin is transferred through exosomes and, upon transfer, has the ability to support functional aberrations in recipient cells. Here, for the first time, it is demonstrated that αvβ3 is present in exosomes released from metastatic PC3 and CWR22Pc prostate cancer cells. Exosomal β3 is transferred as a protein from donor to nontumorigenic and tumorigenic cells as β3 protein or mRNA levels remain unaffected upon transcription or translation inhibition in recipient cells. Furthermore, it is shown that upon exosome uptake, de novo expression of an αvβ3 increases adhesion and migration of recipient cells on an αvβ3 ligand, vitronectin. To evaluate the relevance of these findings, exosomes were purified from the blood of TRAMP mice carrying tumors where the expression of αvβ3 is found higher than in exosomes from wild-type mice. In addition, it is demonstrated that αvβ3 is coexpressed with synaptophysin, a biomarker for aggressive neuroendocrine prostate cancer. IMPLICATIONS: Overall this study reveals that the αvβ3 integrin is transferred from tumorigenic to nontumorigenic cells via exosomes, and its de novo expression in recipient cells promotes cell migration on its ligand. The increased expression of αvβ3 in exosomes from mice bearing tumors points to its clinical relevance and potential use as a biomarker. Mol Cancer Res; 14(11); 1136-46. ©2016 AACR

Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram by mass spectrometry

Secreted fungal proteins with mannanase activity were identified by mass spectrometry of bands excised from a Congo Red stained zymogram containing locust bean gum as substrate. This technique circumvents the need to locate corresponding bands on a parallel gel without substrate and provides good accuracy in targeting proteins for identification. © 2009 Elsevier B.V. All rights reserved

Bioproducts From Euglena gracilis: Synthesis and Applications.

In recent years, the versatile phototrophic protist Euglena gracilis has emerged as an interesting candidate for application-driven research and commercialisation, as it is an excellent source of dietary protein, pro(vitamins), lipids, and the β-1,3-glucan paramylon only found in euglenoids. From these, paramylon is already marketed as an immunostimulatory agent in nutraceuticals. Bioproducts from E. gracilis can be produced under various cultivation conditions discussed in this review, and their yields are relatively high when compared with those achieved in microalgal systems. Future challenges include achieving the economy of large-scale cultivation. Recent insights into the complex metabolism of E. gracilis have highlighted unique metabolic pathways, which could provide new leads for product enhancement by genetic modification of the organism. Also, development of molecular tools for strain improvement are emerging rapidly, making E. gracilis a noteworthy challenger for microalgae such as Chlorella spp. and their products currently on the market

Thermal and non-thermal nature of the soft excess emission from Sersic 159-03 observed with XMM-Newton

Several nearby clusters exhibit an excess of soft X-ray radiation which cannot be attributed to the hot virialized intra-cluster medium. There is no consensus to date on the origin of the excess emission: it could be either of thermal origin, or due to an inverse Compton scattering of the cosmic microwave background. Using high resolution XMM-Newton data of Sersic 159-03 we first show that strong soft excess emission is detected out to a radial distance of 0.9 Mpc. The data are interpreted using the two viable models available, i.e., by invoking a warm reservoir of thermal gas, or relativistic electrons which are part of a cosmic ray population. The thermal interpretation of the excess emission, slightly favored by the goodness-of-fit analysis, indicates that the warm gas responsible for the emission is high in mass and low in metallicity.Comment: ApJ in pres