Preliminary Characterization of the Probiotic Properties of a Bacterial Strain for Used in Monogastric Nutrition

This study aimed to evaluate some probiotic properties of Bacillus subtilis ATCC 6051a. The phenotypic profile, resistance to pH by simulated gastric juice (pH 2 and 3), bile salts by simulated intestinal fluid, survivability (%), heat and antibiotics tolerance were investigated. The strain is a Gram-positive, rod-shaped bacteria, arranged in short chains or in small irregular pairs with the ability to produce spores. Good viability at pH 2 and 3, with a survival of more than ≥80%, was found. In the presence of bile salts 0.3%, over 4 h, the strain exhibited a survival ≥85%. At 80°C, for 120 min., the strain showed good growth (9.04 log CFU/ml). Results were sensitive to most antibiotics, with a highly susceptible (between 16 – 25 mm) to erythromycin, clindamycin, amoxicillin, chloramphenicol, ciprofloxacin, amikacin and kanamycin. The strain was found to be sensitive to vancomycin, gentamicin, and tetracycline. The present research demonstrated that Bacillus subtilis ATCC 6051a can survive under gastrointestinal conditions, which involves them to future in vitro and in vivo probiotic studies

Preliminary Characterization of the Probiotic Properties of a Bacterial Strain for Used in Monogastric Nutrition

This study aimed to evaluate some probiotic properties of Bacillus subtilis ATCC 6051a. The phenotypic profile, resistance to pH by simulated gastric juice (pH 2 and 3), bile salts by simulated intestinal fluid, survivability (%), heat and antibiotics tolerance were investigated. The strain is a Gram-positive, rod-shaped bacteria, arranged in short chains or in small irregular pairs with the ability to produce spores. Good viability at pH 2 and 3, with a survival of more than ≥80%, was found. In the presence of bile salts 0.3%, over 4 h, the strain exhibited a survival ≥85%. At 80°C, for 120 min., the strain showed good growth (9.04 log CFU/ml). Results were sensitive to most antibiotics, with a highly susceptible (between 16 – 25 mm) to erythromycin, clindamycin, amoxicillin, chloramphenicol, ciprofloxacin, amikacin and kanamycin. The strain was found to be sensitive to vancomycin, gentamicin, and tetracycline. The present research demonstrated that Bacillus subtilis ATCC 6051a can survive under gastrointestinal conditions, which involves them to future in vitro and in vivo probiotic studies

In vitro evaluation of probiotic properties of A lactic acid bacteria isolated from piglet

The aim of this study was to evaluate the probiotic aspects of lactic acid bacteria (LAB) isolated from faeces of a healthy piglet (30-d-old, Topigs). Cultural, morphological and biochemical characteristics by standard API 50 CHL Biomerieux strips of isolated strain was assayed as well. The strain was conserved as Lactobacillus fermentum 1 IBNA 95 and can be found in the collection of INCDBNA. It is a Gram positive, non-spore forming, aerotolerant bacteria. It appears isolated and grew at 37°C in Oxoid MRS broth and MRS agar medium. The functional properties tested were to survive at pH 3.0 and to present resistance at addition of 0.3% bile salts (w/v). The strain was able to survive at a low pH value and to tolerate 0.3% bile salt solution during 3h exposure under according simulated gastrointestinal condition. Lactobacillus fermentum 1 IBNA 95 was presenting some probiotic features, thus can be a promising feed supplement for piglet’s nutrition

Effect of Dietary Extruded Linseed and Walnut Meal Mixture (8:1) on Performance and Plasma Protein Profile in Weaned Piglets

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period

Effects of the dietary millet (Panicum Miliaceum) on some quality characteristics of liver, Longissimus dorsi and Semitendinosus muscle on pigs

The purpose of this study was to evaluate the effect of 25% millet (Panicum miliaceum) as replacement of triticale on some quality characteristics of pig meat. Forty pigs Topigs hybrid (initial body weight 13.58 ± 0.37 kg) used in the trial for 30 days were assigned into two dietary treatments: control diet (M) and experimental diet (E, with 25% millet that replaces triticale from M diet). The pigs were slaughtered (n=3/group) at 81 ± 3 days (final body weight 32 ± 4.5 kg). The samples were taken from the liver, Longissimus dorsi (LD) and Semitendinosus (ST) muscle and analyzed for: pH, colour and texture profile. The pH value of the liver and texture profile of all the tissue samples from E diet were not statistically different comparing to the M diet. Pigs fed E diet had a significantly increased value of redness a* in the liver (>17%) and in the ST (>45.5%) compared to M group. The liver yellowness b* was significantly higher (>53%) in the E group compared to the M group. For all tissue samples, the shear force was significantly affected in group E compared to the M group. In conclusion, the replacement of triticale with millet could be recommended for inclusion in pigs diets, without affecting pig meat quality

Alterations in Essential Fatty Acids, Immunoglobulins (IgA, IgG, and IgM), and Enteric Methane Emission in Primiparous Sows Fed Hemp Seed Oil and Their Offspring Response

This study shows the effects of dietary hemp seed oil on the milk composition, blood immunoglobulins (Ig), and enteric methane (E-CH4) production of primiparous sows, and their offspring’s response at three time points. A bifactorial experiment was conducted for 21 days (d) on 18 primiparous sows (195 ± 3 days old). The sows were fed two diets: (i) a control diet (SO) based on soybean oil (1.6%), with an 18.82 n-6:n-3 polyunsaturated fatty acids (PUFA) ratio; (ii) an experimental diet (HO) based on hemp seed oil (1.6%), with a 9.14 n-6:n-3 PUFA ratio. The milk contained an elevated level of linoleic acids (LA), n-3 FA, and especially alpha-linolenic acids (ALA), while the n-6:n-3 ratio declined using hemp oil. The Ig concentration was higher in colostrum than in milk. In the first few hours, the IgG in the plasma of piglets was more than double that of maternal plasma IgG (+2.39 times). A period effect (p < 0.0001) for IgG concentration in the plasma of piglets was recorded (40% at 10 d, respectively 73% lower at 21 d than 12 h after parturition). However, the sow diet did not affect Ig (p > 0.05). The frequency of diarrhoea declined after about 7 d. The value of the rate of diarrhoea was 6.2% lower in the PHO group. We found a 4.5% decline in E-CH4 in the HO group. Applying multiple linear regression, feed intake, n-6:n-3 ratio, ALA, and lean meat were potential indicators in estimating E-CH4. In conclusion, sow dietary hemp seed oil increased lean meat %, milk EFA, and milk IgM. Significant changes in the other dependent variables of interest (body weight, plasma Igs in sows and offspring, E-CH4 production) were not recorded. There was reduced diarrhoea which shows that EFA could play a therapeutic role in the incidence of diarrhoea and in lowering of E-CH4 emission in sows and progeny. All dependent variables were significantly altered at different time points, except for fat concentration in milk and sow plasma IgG

Silkworm <i>Bombyx mori</i>—Sustainability and Economic Opportunity, Particularly for Romania

The main concerns and challenges of raising silkworms include economic value, mulberry management, biodiversity conservation of genetic resources, and developing highly productive breeds for genetic variety. This study investigated the relationship between the economic relevance of the products generated throughout the value chain, limitations, and opportunities to generate incomes for sericulture farmers, trends, and perspectives worldwide, particularly in Romania. Seventy-seven publications were considered from online databases. The diversification of products generated at each level of the value chain of silkworm rearing and their multipurpose applications impact social and economic life. Hence, silk is well known as a valuable biomaterial for industry, suitable for textile and medicine. There are several arguments to use silkworms in human food even though they are not yet authorized as edible insects at the European level. Thus, as a nutrient-rich by-product, silkworm pupae (extract, cakes, and oil) have medicinal properties and can be used for human and animal nutrition. Sericin, silk fibroin, and chitin are bioactive compounds in cocoons and pupae with pharmacological implications and drug composition, while biomass is suitable for biodiesel and excreta for compost. The farmers’ attitudes and mentality associated with political circumstances influence the perspectives for the sericulture field. Due to the high likelihood of using their products, small-medium-scale farmers might benefit sericulture by identifying new sales marketplaces and finding new beneficiaries for directing their multiple products. The funds allotted by government subventions for supporting this fascinating activity and opportunities for jobs may aid in encouraging to start of a new sericulture business or to contribute developing the existing one

Blood Parameters, Digestive Organ Size and Intestinal Microflora of Broiler Chicks Fed Sorghum as Partial Substitute of Corn

A study was conducted to evaluate the effects of dietary white sorghum (WS), as partial substitute of corn on blood parameters, digestive organ size and intestinal microflora of broilers at 35 d. Cobb 500 broilers (n=400), assigned to 2 groups, were fed with isocaloric and isonitrogenous corn-soybean meal control diets (C) or corn-WS-soybean meal diets (WS), where corn was partially replaced (50%) with WS. At 35 d, sixteen broilers per group were selected for blood sampling, gastrointestinal (GIT) measurements, digesta pH and ileum microflora analysis. There was no effect (P>0.05) of the dietary WS inclusion on blood biochemistry parameters, GIT development and digesta pH of broilers. In our study, dietary WS significantly reduced the ileal population of Enterobacteriaceae (-1.38%; P<0.0001) and E. coli (-1.16%; P=0.020), and increased the Lactobacillus spp. (+1.07%; P=0.014) compared with the C diet. In conclusion, feeding white sorghum, as partial substitute of corn in broiler diets, did not affect blood parameters, digestive organ size and digesta pH, that support the obtained performance. In addition, sorghum had a positive effect of the ileal microflora increasing the beneficial bacterial Lactobacillus spp

NUTRITIONAL QUALITY AND VALORIZATION OF SILKWORM PUPAE (BOMBYX MORI. L) IN POULTRY DIETS – REVIEW

The development of the poultry industry increased the request for conventional feed ingredients (soybean meal and oil, fish meal), leading to higher costs and environmental pressure. In this context, there is an increased interest in finding sustainable alternatives such as protein-rich insect species as innovative ingredients in poultry diets. Sericulture produces a lot of by-products, including silkworm pupae (SWP), a valuable and less expensive alternative protein source. Therefore, this review firstly presents the nutritional value of full-fat and defatted SWP as potential feed ingredients for poultry. The proximate composition of SWP consists of 37.8–82.8% crude protein (with a higher digestibility ranging from 76% to 98%), 2–36.2% crude fat, 1–9.5% crude fibre, 1–10.6% ash, and 21.9–25.8 MJ/kg gross energy. SWP has high-quality protein and lipids due to their essential amino acids (40- 48% of total amino acids content) and fatty acids profiles (especially alpha-linolenic, 20-38% and linoleic, 5.5–21%). The vitamins, minerals, and other bioactive compounds (i.e., 1-Deoxynojirimycin, 1-DNJ) complete their nutritional value. SWP also contains a polysaccharide, chitin (1.5–3.4% of dry matter), with structural function in organisms. Secondly, we reported the effects of using SWP in broilers, laying hens and quails diets, focusing on productive performance, health status and quality of poultry products. The findings demonstrated that SWP could partially replace conventional ingredients in poultry diets, maintaining appropriate performance and/or improving meat or egg qualities

Effects of Microencapsulated Probiotics on Performance, Organ Development, Diarrhoea Incidences, Blood Parameters, Intestinal Histomorphology and Microflora in Weaning Piglets

The study aimed to assess the effects of the dietary supplementation of microencapsulated L. acidophilus and L. plantarum and their combination on the growth performance, organ development, diarrhoea incidences, blood profiles, intestinal histomorphology and microflora in weaned piglets. For that, 160 piglets with an average body weight (BW) of 8.52 ± 0.15 kg were divided into four groups (40 piglets/group) and allotted to one of the four dietary treatments as follows: a basal diet (C diet) or a basal diet containing 1 × 108 CFU/g of L. acidophilus (LA diet), or a diet containing 3 × 108 CFU/g of L. plantarum (LP diet) and a diet with the combination of both bacterial strains (LA + LP diet) for 21 days. On day 14, probiotics significantly increased ADFI, while FCR was higher in the LA and LP groups than the C and LA + LP groups. No effects (p > 0.05) on visceral organs weight, intestinal pH and biochemical parameters among treatments were noticed. Treatments significantly lowered diarrhoea incidence compared to control. Villus width was greater (p p p Lactobacillus spp. while decreasing the populations of Escherichia coli and Staphylococcus. Our results indicated that dietary supplementation of microencapsulated probiotics, particularly the combination of L. plantarum and L acidophilus strains, maintained growth performance, lowered diarrhoea incidence and beneficially altered the intestinal architecture and microbial populations of weaned piglets