31 research outputs found
Alignment of plancitoxin-1-like proteins.
<p>The aligned sequences are as follows: <i>Acanthaster planci</i> plancitoxin 1 (BAD12432), <i>Saccoglossus kowalevskii</i> plancitoxin 1-like (XP_006823536), <i>T</i>. <i>spiralis</i> plancitoxin1-like protein (AHM10158) and <i>Hydra vulgaris</i> plancitoxin 1-like (XP_004209299). Black and gray indicate amino acids that are identical or highly conserved, respectively, across all aligned sequences.</p
Additional putative toxin transcripts identified by similarity searches.
<p><sup>a</sup> Full-length and not full-length open reading frames (ORFs) are indicated by “F” and “N”, respectively.</p><p><sup>b</sup> Transcripts with or without a signal peptide are indicated by “Y” and “N”, respectively; transcripts in which the presence of a signal peptide was unclear are indicated by “U” (Unknown).</p><p>Additional putative toxin transcripts identified by similarity searches.</p
Categorization of unigenes into KEGG biochemical pathways.
<p>A total of 13,663 unigenes were assigned to 241 KEGG pathways belonging to six categories. (A) The percentage of the pathway amount in each category is shown. (B) The ten largest groups with KEGG database annotation. The x-axis indicates the number of annotated unigenes.</p
Alignment of the conserved sequence motifs of Kunitz-type inhibitors.
<p>The deduced amino acid sequences of unigenes 18473, 23096, 40962 and 40624 were aligned with known Kunitz-type inhibitors, including <i>Ixodes scapularis</i> Kunitz-domain protein (XP_002435213), <i>Crassostrea gigas</i> putative Kunitz-type proteinase inhibitor (EKC39386), <i>Latrodectus hesperus</i> Kunitz-like protease inhibitor (ADV40132), <i>Astyanax mexicanus</i> Kunitz-type protease inhibitor 1-like (XP_007252976) and <i>Danio rerio</i> Kunitz-type protease inhibitor 1 (AAI63937). The six highly conserved cysteine residues are indicated by asterisks.</p
Alignment of translationally controlled tumor protein (or histamine-releasing factor).
<p>The aligned sequences are as follows: <i>Rhipicephalus microplus</i> histamine release factor (AAY67698), <i>Amblyomma americanum</i> histamine release factor (AAY67700), <i>Latrodectus hesperus</i> histamine release factor (ADV40083), <i>Saccoglossus kowalevskii</i> TCTP (XP_002740226) and <i>Ixodes scapularis</i> TCTP (AAY66972). Black and gray indicate amino acids that are identical or highly conserved across all aligned sequences, respectively.</p
Global Transcriptome Analysis of the Tentacle of the Jellyfish <i>Cyanea capillata - Fig 5 </i> Using Deep Sequencing and Expressed Sequence Tags: Insight into the Toxin- and Degenerative Disease-Related Transcripts
<p><b>(A) Multiple sequence alignment of the amino acid sequence of unigene 6213 with other known CfTX-like cnidarian toxins.</b> The abbreviations and sequence accession numbers for the aligned sequences are as follows: <i>Aurelia aurita</i> TX2 (AFK76349), <i>Chiropsalmus quadrigatus</i> CqTX-A (BAB82520), <i>Carybdea rastonii</i> CrTX-A (BAB12728), <i>Carybdea alata</i> CaTX-A (BAB12727), and <i>Chironex fleckeri</i> CfTX-A, CfTX-B, CfTX-1 and CfTX-2 (AFQ00676, AFQ00677, ABS30940 and ABS30941, respectively). The putative highly conserved transmembrane spanning region (TSR1) in the N-terminal sequences of this toxin family is boxed. (B) Phylogenetic relationships of the CfTX-like cnidarian toxins.</p
Alignment of the amino acid sequence of unigene 41308 with <i>Mesobuthus eupeus</i> venom toxin-like peptide-6 and venom protein 2.
<p>The GenBank accession numbers for venom toxin-like peptide-6 and venom protein 2 are ABR21046 and ABR21036, representatively. Black and gray indicate amino acids that are identical or highly conserved across all aligned sequences, respectively.</p
Top-hit species distribution of the BLASTx matches of the unigenes.
<p>The species distributions of the top BLASTx hits against the Nr database were analyzed (cut-off e-value < 10<sup>−5</sup>). Species with proportions of greater than 1% are shown.</p
Multiple sequence alignment of the amino acid sequence of unigenes 5396 and 5817 with those of other known huntingtin proteins.
<p>The aligned sequences are as follows: <i>Hydra vulgaris</i> huntingtin-like (XP_004206850), <i>Saccoglossus kowalevskii</i> huntingtin (XP_006822985), <i>Poecilia reticulate</i> huntingtin isoform X 5 (XP_008406720), <i>Pongo abelii</i> huntingtin (XP_002814571), <i>Oryctolagus cuniculus</i> huntingtin (XP_008248081), <i>Python bivittatus</i> huntingtin (XP_007432531) and <i>Branchiostoma floridae</i> huntingtin (ABP04240).</p
Summary of the <i>C</i>. <i>capillata</i> tentacle transcriptome.
<p>Summary of the <i>C</i>. <i>capillata</i> tentacle transcriptome.</p