Novel Concepts of “Niche-Relief” and Niche-Voltage” for Stem Cells as a Base of Bone and Hematopoietic Tissues Biomimetic Engineering

An affect of relief features and quantitative variables of artificial surfaces on structural-functional status of human lung prenatal stromal cells (HLPSC) and remodeling of Balb/c mice bone/bone marrow system have been studied. Implants with rough (Ra>2 μm) calcium phosphate micro-arc coatings have structural-functional sites (micro-regions) named “niches-relief” which are necessary for maturation and differentiation of HLPSC into secreting osteoblasts in shortterm culture. Maximal remodeling of mouse bone/bone marrow system in 45-day subcutaneous heterotopic test in vivo is also noted under optimal parameter (average index of cellular alkaline phosphatase area to artificial micro-region area is about 43 %) of osteogenic niche in vitro. Probable physical mechanism of osteogenic niche functioning has been determined. It correlates with increasing of electron work function supplied with a negative charge of calcium phosphate nanorelief in the sockets (artificial “niches”). “Niche-voltage” concept for bone tissue biomimetic engineering was proposed

Novel Concepts of “Niche-Relief” and Niche-Voltage” for Stem Cells as a Base of Bone and Hematopoietic Tissues Biomimetic Engineering

Currently, a lot of publications, mainly as hypotheses, were dedicated to niches for hemopoietic stem cells (HSC). Multipotent mesenchymal stromal cells (MMSC) are treated as important cell component of HSC niches [1]. At the same time, the circumstance that discussion of existence of niches determining fate of MMSC themselves just appears in literature sets at gaze [2]. Pilot experimental data about existence and dimensions of niches for osteogenic differentiation of MMSC, their close dependence on quantitative variables of bone mineral substance relief were recently obtained by us [3]. In this connection, investigation of the relief features effects of model mineral matrix on structural-functional conditions of human MMSC and remodeling of bone/bone marrow system in mice was of great interest