15,417 research outputs found
The Impact of the Long-Distance Transport of a BEL1-Like Messenger RNA on Development
BEL1- and KNOTTED1-type proteins are transcription factors from the three-amino-loop-extension superclass that interact in a tandem complex to regulate the expression of target genes. In potato (Solanum tuberosum), StBEL5 and its Knox protein partner regulate tuberization by targeting genes that control growth. RNA movement assays demonstrated that StBEL5 transcripts move through the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement to stolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts. Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark, promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor, binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motif in the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolon tips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results in the reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5/POTH1 binding specificity to the motifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggest that, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.Fil: Lin, Tian. University of Iowa; Estados UnidosFil: Sharma, Pooja. University of Iowa; Estados UnidosFil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de AgrobiotecnologĂa del Litoral. Universidad Nacional del Litoral. Instituto de AgrobiotecnologĂa del Litoral; ArgentinaFil: Viola, Ivana Lorena. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de AgrobiotecnologĂa del Litoral. Universidad Nacional del Litoral. Instituto de AgrobiotecnologĂa del Litoral; ArgentinaFil: Hannapel, David J.. University of Iowa; Estados Unido
Water vapor emission from IRC+10216 and other carbon-rich stars: model predictions and prospects for multitransition observations
We have modeled the emission of H2O rotational lines from the extreme C-rich
star IRC+10216. Our treatment of the excitation of H2O emissions takes into
account the excitation of H2O both through collisions, and through the pumping
of the nu2 and nu3 vibrational states by dust emission and subsequent decay to
the ground state. Regardless of the spatial distribution of the water
molecules, the H2O 1_{10}-1_{01} line at 557 GHz observed by the Submillimeter
Wave Astronomy Satellite (SWAS) is found to be pumped primarily through the
absorption of dust-emitted photons at 6 m in the nu2 band. As noted by
previous authors, the inclusion of radiative pumping lowers the ortho-H2O
abundance required to account for the 557 GHz emission, which is found to be
(0.5-1)x10^{-7} if the presence of H2O is a consequence of vaporization of
orbiting comets or Fischer-Tropsch catalysis. Predictions for other
submillimeter H2O lines that can be observed by the Herschel Space Observatory
(HSO) are reported. Multitransition HSO observations promise to reveal the
spatial distribution of the circumstellar water vapor, discriminating among the
several hypotheses that have been proposed for the origin of the H2O vapor in
the envelope of IRC+10216. We also show that, for observations with HSO, the
H2O 1_{10}-1_{01} 557 GHz line affords the greatest sensitivity in searching
for H2O in other C-rich AGB stars.Comment: 35 pages, 12 figures, to be published in The Astrophysical Journa
11th Annual Idaho Public Policy Survey
The Policy Survey attempts to identify public policy issues that are of concern to Idaho citizens. This report is the primary vehicle for dissemination of public policy concerns to the State Legislature, state agencies, and to the public at large.
Since its inception in 1990, a set of questions referred to as core questions have been asked each year. Responses to these core questions on the: quality of life in Idaho; problems facing Idaho; perceptions, trust and confidence in government and taxing entities; and perceptions on funding-levels for programs and services-are important indicators of changes in attitudes and opinions of the citizens of Idaho.
This year the State Department of Parks and Recreation, the State Division of Vocational Education, and BSU\u27s Department of Public Policy and Administration sponsored questions that were of interest to each respective organization. Also this year, two additional questions asked Idaho citizens their source of information and influence on their opinions on public policy concerns
Electric-field tuning of the valley splitting in silicon corner dots
We perform an excited state spectroscopy analysis of a silicon corner dot in
a nanowire field-effect transistor to assess the electric field tunability of
the valley splitting. First, we demonstrate a back-gate-controlled transition
between a single quantum dot and a double quantum dot in parallel that allows
tuning the device in to corner dot formation. We find a linear dependence of
the valley splitting on back-gate voltage, from to with a slope of (or equivalently a slope
of with respect to the effective field). The
experimental results are backed up by tight-binding simulations that include
the effect of surface roughness, remote charges in the gate stack and discrete
dopants in the channel. Our results demonstrate a way to electrically tune the
valley splitting in silicon-on-insulator-based quantum dots, a requirement to
achieve all-electrical manipulation of silicon spin qubits.Comment: 5 pages, 3 figures. In this version: Discussion of model expanded;
Fig. 3 updated; Refs. added (15, 22, 32, 34, 35, 36, 37
Adaptation of the Conditioned Assessment of Speech Production in Spanish
Purpose: The purpose of this article is to describe the adaptation of the Spanish adaptation of the CASP.
Method: The authors adapted each segment into Spanish, then had 41 participants complete a survey to determine if each adapted segment was representative of the Spanish phonologic system. Thirty-six children (half with typical hearing, half with hearing loss) completed the CASP in English and Spanish. Paired samples t-tests were run to compare English and Spanish CASP scores between children with hearing loss and those with typical hearing.
Results: All segments were adapted as needed into Spanish. There was no statistical difference between the English CASP scores (18.61 ± 2.03) and Spanish CASP scores (18.78 ± 1.99) for the children with typical hearing. Similarly, there was no statistical difference between the English CASP scores (16.78 ± 3.44) and Spanish CASP scores (16.67 ± 3.41) for the children with hearing loss. Children with typical hearing scored statistically significantly higher on the English and Spanish CASP than children with hearing loss.
Discussion: The CASP-S is an appropriate Spanish adaptation of the CASP, which has been field-tested for use with young Spanish-speaking children with hearing loss
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