Lady Lazarus Has Wheels: poem and note

A poem inspired by Kurt Schwitters, Sylvia Plath, and W.B. Yeats, followed by a note on its conception and composition

Lady Lazarus Has Wheels: poem and note

A poem inspired by Kurt Schwitters, Sylvia Plath, and W.B. Yeats, followed by a note on its conception and composition

Into the Orchid House with Virginia Woolf

An examination of the queer sexual textual politics of orchids in the writings of Virginia Woolf, including 'Kew Gardens' and Night and Day, with reference to suffragette activism and the orchidaceous aesthetics of Oscar Wilde

Parks, people and pixels: evaluating landscape effects of an East African national park on its surroundings

Landscapes surrounding protected areas, while still containing considerable biodiversity, have rapidly growing human populations and associated agricultural development in most of the developing world that tend to isolate them, potentially reducing their conservation value. Using field studies and multi-temporal Landsat imagery, we examine a forest park, Kibale National Park in western Uganda, its changes over time, and related land cover change in the surrounding landscape. We find Kibale has successfully defended its borders and prevents within-park deforestation and other land incursions, and has maintained tree cover throughout the time period of the study. Outside the park there was a significant increase in tea plantations and continued forest fragmentation and wetland loss. The question of whether the park is a conservation success because of the network of forest fragments and wetlands or in spite of them remains unanswered

Editing Woolf

No abstract available

Editing Woolf

No abstract available

Characterization of Recombination Events Leading to the Production of an Ecotropic Replication-Competent Retrovirus in a GP+envAM12-Derived Producer Cell Line

AbstractReplication-competent retrovirus (RCR) was identified in a GP+envAM12-derived producer cell, containing the MFG-S-Neo retroviral vector, using a marker rescue assay. Studies were undertaken to determine the origin and structure of this RCR. Receptor interference assays demonstrated that the virus was pseudotyped with an ecotropic envelope. Molecular analysis demonstrated the presence of a MoMLV ecotropic env recombinant where the neomycin resistance gene of the MFG-S-Neo vector was replaced by MoMLV ecotropic env. Additional recombinants linking the retroviral pol gene to neo and the neo gene to MoMLV env were also identified. A full-length MoMLV retroviral genome was detected by nested PCR in the contaminated amphotropic producer cells and in cells infected with its supernatant. Unexpectedly, this was also present in the GP+E86 packaging cells together with a previously undescribed envelope construct possessing a full 5′ and 3′ LTR, although these cells were consistently negative for the presence of RCR. These anomalies in the GP+E86 packaging cell line result in increased homology with the MFG-S-Neo vector, leading to an increased risk for the production of RCR. Our findings point to a need for increased vigilance when using these packaging lines to generate replication-defective retrovirus

Uncomplicated Monochorionic Diamniotic Twins and the Timing of Delivery

Cleary-Goldman and d'Alton discuss the implications of a new study in PLoS Medicine examining the risk of fetal death in uncomplicated monochorionic diamnotic twin pregnancies