Large Follicular Cyst Associated with Upper Third Molar in the Maxillary Sinus with Pterygomaxillary Space Extension: Reflection on a Case Report

BACKGROUND: Follicular cysts are osteolytic lesions characterized by greater growth, differentiation, and degeneration potentials, compared to inflammatory root cysts. Those connected to upper third molars can disrupt the neighboring teeth and extend to maxillary sinus and adjacent anatomical structures. CASE PRESENTATION: In this study, we present a case of a large dimension follicular cyst associated with the upper third molar, extended to maxillary antrum and pterygomaxillary space, uncommon for dimensions, involving meso- and sovra-structure describing the clinical diagnostic procedures, and the surgical strategies adopted. The cone-beam computed tomography allowed a very accurate analysis of the lesion. Surgical excision was performed through Caldwell-Luc technique, approaching to the pterygomaxillary space. A 1-year follow-up shows good clinical, anatomical, and functional conditions. Histological examination confirmed the diagnosis of follicular cyst. CONCLUSION: Giant follicular cysts require an accurate pre-operative study due to the delicate structures that may be involved

NPBTs FOR SUSTAINABLE VITICULTURE MANAGEMENT TO BIOTIC AND ABIOTIC STRESS

New plant breeding techniques (NPBTs) aim to overcome traditional breeding limits for plant improvement to biotic and abiotic stresses satisfying the European Policies requirements that promote chemical input reduction and a more sustainable agriculture. We decided to apply genome editing (via CRISPR/Cas9) focusing on susceptibility genes to control powdery mildew: we chosen to knock-out two genes belonging to MLO (Mildew Locus O) family: VvMLO7 and VvMLO6. The same approach was used to cope with abiotic stresses, in specifc drought, performing a knock-out of four genes, two belonging to GST (Glutathione S-Transferase) and two to PME (Pectin Methyl Esterase) gene families. In parallel to genome editing, we also applied cisgenesis to move the resistance locus RPV3-1 (Resistance to Plasmopara viticola) into economically important cultivars. This locus is formed by two di\ufb00erent genes that were inserted individually and in combination to evaluate their e\ufb00ects. One of the drawbacks linked to classical Agrobacterium tumefaciens mediated transformation is the insertion of unrelated transgene (e.g., antibiotic resistance). These markers are required for transgenic plants selection, but undesirable to be retained in commercial plants due to possible toxicity or allergenicity to humans and animals, in addition to their potential hazards for the environment. To overcome these limits, we exploit an inducible excision system based on a Cre-lox recombinase technology controlled by a heat-shock inducible promoter that will be activated once the transformation event(s) will be confrmed. Embryogenic calli of Chardonnay, Glera, Microvine, Pinot Noir, Sangiovese, were used in stable transformation with A. tumefaciens carrying the genome editing construct with the MLO-guideRNAs and the cisgenic construct carrying the two RPV3-1 genes. Embryogenic calli of rootstocks 110 Richter and SO4 were transformed with genome editing construct carrying GST and PME guideRNAs in two independent transformations. Regenerated embryos from all the transformation events are now under evaluation

New plant breeding technologies towards a more sustainable viticulture

European grapevine cultivars are highly susceptible to many pathogens that are managed through large pesticide use. Nevertheless, the European policies promote pesticide use reduction and new environmentally friendly methods for a more sustainable agriculture. In this framework, grapevine genetic improvement could benefit from New Plant Breeding Technologies. In order to reduce fungal susceptibility, we will produce knock-out plants from embryogenic calli using CRISPR/Cas9 technology. Studies in barley reported the acquisition of powdery mildew resistance by knocking out susceptibility genes belonging to the MLO (Mildew Locus O) family. In this study, our approach takes advantage from CRISPR/Cas9 technology to perform a multiple knockout of MLO genes. Among the 17 VvMLOs reported in grapevine we designed constructs to target VvMLO6 and VvMLO7. Golden Gate assembly was used to produce three different constructs (containing two guideRNAs for each gene) to knocking-out the targets singularly or by producing a double mutant. Usually, the genetic engineering techniques, mediated by A. tumefaciens, involve the insertion of exogenous selectable marker genes. These markers are required for selection of transgenic plants, but they are undesirable to be retained in commercial transgenic plants due to possible toxicity or allergenicity to humans and potential environmental hazard. To overcome these limits, we opted for a \u201cclean\u201d editing strategy developing an inducible excision system. This approach is based on a recombinase technology involving the Cre-loxP system from the P1 bacteriophage under a heat-shock inducible promoter to be activated once the editing event(s) will be confirmed. Obtainment of embryogenic calli is one of the main bottlenecks for application of CRISPR/Cas9: for two seasons, we collected inflorescences from Chardonnay, Glera, Microvine, Pinot Noir, Sangiovese cultivars and two rootstocks, 110 Richter and SO4, cultured and maintained in vitro up to embryo development and then used to perform Agrobacterium tumefaciens GV3101 mediated transformation

Early detection of hip periprosthetic joint infections through CNN on Computed Tomography images

Early detection of an infection prior to prosthesis removal (e.g., hips, knees or other areas) would provide significant benefits to patients. Currently, the detection task is carried out only retrospectively with a limited number of methods relying on biometric or other medical data. The automatic detection of a periprosthetic joint infection from tomography imaging is a task never addressed before. This study introduces a novel method for early detection of the hip prosthesis infections analyzing Computed Tomography images. The proposed solution is based on a novel ResNeSt Convolutional Neural Network architecture trained on samples from more than 100 patients. The solution showed exceptional performance in detecting infections with an experimental high level of accuracy and F-score

Cox regression and survival analysis from the tauro-urso-deoxycholic trial in amyotrophic lateral sclerosis

Recent phase II pilot clinical trials suggested that tauro-urso-deoxycholic acid (TUDCA) might slow functional decline and increase survival in patients with amyotrophic lateral sclerosis (ALS). We performed a multivariate analysis of the original TUDCA cohort to better define the treatment effect and allow comparability with other trials. Linear regression slope analysis showed statistical differences in the decline rate, favoring the active treatment arm (p-value < 0.01; −0.262 for the TUDCA group and −0.388 for the placebo group). Mean survival time, estimated by the Kaplan–Meier analysis, showed a 1-month difference, favoring active treatment (log-rank test p-value = 0.092). Cox regression analysis demonstrated that placebo treatment was associated with a higher risk of death (p-value = 0.055). These data further support the disease-modifying effect of TUDCA monotherapy and raise the question of what could be the additional effect of combining TUDCA with sodium phenylbutyrate

Molecular Imaging Diagnosis of Renal Cancer Using 99mTc-Sestamibi SPECT/CT and Girentuximab PET-CT-Current Evidence and Future Development of Novel Techniques

: Novel molecular imaging opportunities to preoperatively diagnose renal cell carcinoma is under development and will add more value in limiting the postoperative renal function loss and morbidity. We aimed to comprehensively review the research on single photon emission computed tomography/computed tomography (SPECT/CT) and positron emission tomography computed tomography (PET-CT) molecular imaging and to enhance the urologists' and radiologists' knowledge of the current research pattern. We identified an increase in prospective and also retrospective studies that researched to distinguish between benign and malignant lesions and between different clear cell renal cell carcinoma subtypes, with small numbers of patients studied, nonetheless with excellent results on specificity, sensitivity and accuracy, especially for 99mTc-sestamibi SPECT/CT that delivers quick results compared to a long acquisition time for girentuximab PET-CT, which instead gives better image quality. Nuclear medicine has helped clinicians in evaluating primary and secondary lesions, and has lately returned with new and exciting insights with novel radiotracers to reinforce its diagnostic potential in renal carcinoma. To further limit the renal function loss and post-surgery morbidity, future research is mandatory to validate the results and to clinically implement the diagnostic techniques in the context of precision medicine

Effect of Parity on Claw Horn Lesions in Holstein Dairy Cows: Clinical and Radiological Study

AbstractThe aim of this study was to estimate the effect of parity on claw lesions prevalence in Holstein dairy cows through clinical and radiological observations. Ninety-seven cows were examined two months after calving to record body condition score, locomotion score, feet diagnostic imaging (X-ray), hoof trimming and clinical evaluation. According to the lactation, the cows were divided into three groups: first parity (Group 1, n=40), second parity (Group 2, n= 29) and third or more parity (Group 3, n=28). Several claw lesions were found. One-way analysis of variance was applied along with Pearson's correlation coefficient. Significant effect of groups was evidenced on radiographic images. In particular, the radiographic images showed a significant increase of osteolysis from Group 1 to Group 3. The application of Pearson's correlation coefficient showed a significantly positive correlation between locomotion score and some claw lesions in all groups. In conclusion, this study provides an overview of claw lesions in dairy cow through clinical and radiological observations

Organic Consumers’ Viewpoints Towards New Breeding Techniques In Italy

Despite the recent decision of the European Court of Justice to consider plant obtained by New Breeding Techniques (NBTs) as GMOs, there is still an intensive debate in Europe on the use of these new techniques in the organic farming. For this reason, understanding organic consumers’ viewpoint towards NBTs is essential for the future of the sector. Following the Q method approach (Brown, 1980), a pilot study was conducted in Italy with 36 organic consumers. The balanced Q-sample, which accounted 48 statements regarding NBTs, was defined according to a 4 x 2 factorial design with six replications. Results demonstrated the presence of two relevant viewpoints towards the adoption of the NBTs in the organic seed and plant breeding sector