Statistical comparisons between embryonic death rates of IRCS.

<p>The mean of embryonic death rate (±SEM) for (n) gestations is presented for four substrains containing different regions of spretus origin: R6 (containing <i>Led2minA</i> only), R3 and R5 (containing Led2minA and Led2minB), and R4 (Led2minB only).</p

Microarray validation by RT-QPCR on 7 genes of the QTL region.

<p>Microarray validation by RT-QPCR on 7 genes of the QTL region.</p

Gene list in the minimal <i>Led2min</i> region.

<p>Gene list in the minimal <i>Led2min</i> region.</p

Number of transcripts modified in uterus of R3 IRCS compared to C57BL6/J.

<p>Number of transcripts modified in uterus of R3 IRCS compared to C57BL6/J.</p

Genomic structure of the IRCS mice in the region of interest of chromosome 1.

<p>The map presents the genomic background, spretus or musculus, of the 7 recombinant substrains (Rc) in the chromosomal region corresponding to <i>Led2</i> QTL. Recombinant strains were generated at the Pasteur institute (Paris) from 66HMMU1 strain by recombination events inside the MMU1 <i>spretus</i> segment. These strains have been genotyped using 24 polymorph markers. Marker positions are given in megabase pairs (Mb). “S" corresponds to the marker in a spretus homozygous form and “B" to the marker in a musculus (B6) homozygous form. The two minimal <i>spretus</i> regions (<i>Led2minA</i> with main effect and <i>Led2minB</i> with probable weak effect) responsible for the phenotype of interest are highlighted in gray and in gray hatched when coexisting in the same substrain.</p

Embryonic resorption rate in function of the type of crossing realized with IRCS and B6 mice.

<p>The results of different crosses (♀IRCS × ♂B6, ♀B6 × ♂IRCS and ♀B6 × ♂B6) are presented as the average (±SEM) of the embryonic resorption rate for (n) gestations.</p

Genes of <i>Led2min</i> region expressed in uterus (>500 AUF) and displaying a deregulation (R3/B6 ratio) and/or non synonymous SNP.

<p>ND: Not determined.</p

Statistical analysis of the embryonic resorption phenotype.

<p>Comparison between IRCs and B6 control using t-test with Bonferroni-corrected level.</p><p>(NS: non-significant).</p

Table S2 from Association of <i>FOXD1</i> variants with adverse pregnancy outcomes in mice and humans

Primer sequence

Figure S1 from Association of <i>FOXD1</i> variants with adverse pregnancy outcomes in mice and humans

FKHD binding sites are more abundant in placenta down-regulated genes in the 66H-Chr13 strai