Environmental distribution of bacterial orders.

<p>Canonical correspondence analysis of sampling sites libraries from JM(e), BL(e) and JOA(e). Environmental parameters included in this analysis are: total phosphorus (Ptotal), ammonia (NH₃), turbidity (Turb), nitrite (NO₂⁻), temperature (Temp), salinity (Sal), pH, nitrate (NO₃⁻), dissolved oxygen (OD) and orthophosphate (PO₄⁻).</p

Match and similarities between bacterial communities.

<p>Principal coordinates plots (PCoA) were generated using the pairwise unweighted UniFrac distances. (<b>A</b>) Comparison between libraries from bulk-environmental DNA (BE), enrichment Cultures (EC) and isolates. (<b>B</b>) Comparison between bulk-environmental DNA libraries (e) of marine (JOA), brackish (BL) and freshwater (JM) sampling points, and (<b>C</b>) Comparison between enrichment culture libraries (c) of marine (JOA), brackish (BL) and freshwater (JM) sampling points. Venn diagrams (<b>D</b>) from libraries of bulk-environmental DNA (BE), enrichment cultures (EC) and isolates. (<b>E</b>) Venn diagrams from bulk-environmental DNA libraries (e) of marine (JOA), brackish (BL) and freshwater (JM) sampling points, and (<b>F</b>) Venn diagrams from enrichment culture libraries (c) of marine (JOA), brackish (BL) and freshwater (JM) sampling points.</p

Phylogenetic tree of bacterial clones from BHI enriched culture.

<p>Reference sequences from GenBank showcased in bold. OTUs were defined by using a distance level of 3% by using the furthest neighbor algorithm in MOTHUR. Access number from each OTU is displayed. Tree topology is based on neighbor joining and bootstrap analysis was performed with 1000 replications. Bootstrap value <50 are not shown.</p

Bacterial taxonomic classes in environmental and cultured libraries.

<p>Sequences obtained from 16S rRNA gene libraries retrieved from environmental samples (black) and from enrichment cultures (grey) taxonomic assignment was performed through the RDP Classifier tool.</p

Phylogenetic tree of bacterial clones from environmental bulk DNA.

<p>Reference sequences from GenBank are shown in bold. OTUs were defined by using a distance level of 3% by the furthest neighbor algorithm in MOTHUR. Access number from each OTU is displayed. Tree topology is based on neighbor joining and bootstrap analysis was performed with 1000 replications. Bootstrap value <50 are not shown.</p

Physico-chemical data from sampling stations.¹

*<p><i>MiliSiemens</i> – mS/cm; ** Nefelométric Turbidity Unity – NTU; *** mg/L.</p>1<p>Additional information on physico-chemical parameters of the sampling sites can be found at INEA (<a href="http://www.inea.rj.gov.br" target="_blank">http://www.inea.rj.gov.br</a>).</p

Sampling sites and experimental strategy.

<p>Stars (<>\vskip -1 \scale 60%\raster="rg1"<>) mark sampling points in the lagoon system.</p

DGGE analysis of environmental and cultured samples.

<p>Dendogram generated using Bionumeric software based on images of DGGE band pattern profiles. Each sample is identified as environmental (e) or cultured (c).</p

Phylogenetic tree of isolated bacteria.

<p>Reference sequences from GenBank are showed in bold. OTUs were defined by using a distance level of 3% by the furthest neighbor algorithm in MOTHUR. Access number from each OTU is displayed. Tree topology is based on neighbor joining and bootstrap analysis was performed with 1000 replications. Bootstrap values <50 are not shown.</p