The Interrelation of the Adrenal and Posterior Pituitary Glands in Water and Electrolyte Metabolism

The secretions of the adrenal medulla account for many of the instantaneous responses of the animal body to various stimuli, but it has not been established that such secretions play any significant role in the endocrine regulation of salt and water metabolism. On the other hand, it has been established that the adrenal cortex and the antidiuretic hormone of the posterior pituitary are intimately involved in such processes. Certain compounds of the amine type, among which are the adrenal medullary hormone, do have a dramatic action on water diuresis; but it is questioned whether these substances are involved in the physiological processes involving normal water metabolism (Eversole, et al., 1940,1942,1952; Gaunt, et al., 1945, 1949; Stein and Wertheimer, 1944)

An Intravenous Assay for Antidiuretic Substance

In recent years it has been our desire to evaluate the presence of antidiuretic substance (ADS) in the bloods of animals under various experimental conditions (Giere, 1951; Eversole, Giere and Rock, 1952; Giere and Eversole, 1954; Eversole and Giere, 1954). For these determinations we have used the method of Birnie, et al. (1950) and also Ames and van Dyke\u27s (1952) modification of the intravenous method of Jeffers, Livezey and Austin (1942). Although these technics are qualitatively accurate it has been our experience that they are oftentimes difficult to duplicate. The following technic is offered as a semi-quantitative procedure which can be duplicated by a practiced individual

Antidiuretic Substance Levels in Rat plasma

In recent years it has been our desire to evaluate the presence of antidiuretic substance (ADS) in the blood of animals under various experimental conditions (Giere, 1951; Eversole, Giere and Rock, 1952; Giere and Eversole, 1954; Eversole and Giere, 1954). For these determinations we have used the method of Birnie, et al. (1950) and also our modification of the technic of Ames and van Dyke (1952). The results herein reported constitute a continuation of the above work to include other physiological and experimental conditions including such controversial subjects as the concentration of ADS in the adrenalectomized rat (Birnie, et al., 1950; Ames and van Dyke, 1952; Robinson and McFarlane, 1956)

Adrenal Ascorbic Acid Depletion Without Eosinopenia in Hypophysectomized Rats Injected lntrasplenically with Corticotrophin

Several workers (Greenspan et al., 1950; Reinhardt and Li, 1951; Stack-Dunne and Young, 1951) have reported a lack of correlation between the eosinopenic, adrenal ascorbic acid lowering activity, and maintenance of adrenal weights with various adrenocorticotrophic preparations. Reinhardt et al. (1951) have critically evaluated assay methods for adrenocorticotrophic hormone (ACTH) and cautioned that differences in rate of absorption, variations in route of administration and dose-time-response relationships may influence the results obtained with different testing procedures. The study reported here was conducted under standardized conditions to determine whether the route of administration would influence the eosinopenic and adrenal ascorbic acid depleting properties of two ACTH preparations