1 research outputs found
DrugāDrug Interactions within Protein Cavities Probed by TripletāTriplet Energy Transfer
A new direct and
noninvasive methodology based on transient absorption
spectroscopy has been developed to probe the feasibility of drugādrug
interactions within a common protein binding site. The simultaneous
presence of (<i>R</i>)-cinacalcet (CIN) and (<i>S</i>)-propranolol (PPN) within human or bovine Ī±<sub>1</sub>-acid
glycoproteins (AAGs) is revealed by detection of <sup>3</sup>CIN*
as the only transient species after laser flash photolysis of CIN/PPN/AAG
mixtures at 308 nm. This is the result of tripletātriplet energy
transfer from <sup>3</sup>PPN* to CIN, which requires close contact
between the two drugs within the same biological compartment. Similar
results are obtained with nabumetone and CIN as donor/acceptor partners.
This new methodology can, in principle, be extended to a variety of
drug/drug/biomolecule combinations