Fatty acid profile in adipose tissue of Non-Maternally Separated rats fed un-supplemented diet (A), Maternally Separated rats fed un-supplemented diet (B), Non-Maternally Separated rats fed <i>B. breve</i> DPC6330 (C), Maternally Separated rats fed <i>B. breve</i> DPC6330 (D), Non-Maternally Separated rats fed <i>B. breve</i> DPC6330 plus 0.5% (w/w) linoleic acid and 0.5% (w/w) α-linolenic acid (E), Maternally Separated rats fed <i>B. breve</i> DPC6330 plus 0.5% (w/w) linoleic acid and 0.5% (w/w) α-linolenic acid (F) for 7 weeks.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048159#s3" target="_blank">Results</a> are expressed as percentage of total identified fatty acids. Data are Means ± SEM g/100 g FAME.</p><p>^{A, B, C, D, E, F} Different superscript letters within a column indicate significant difference (* = p<0.05, ** = p<0.01, *** = p<0.001). FAME = fatty acid methyl esters. C16:0 palmitic acid; C16:1c9 palmitoleic acid; C18:0 stearic acid; C18:1<i>c</i>9 oleic acid; C18:1<i>t</i>9 elaidic acid<b>;</b> C18:2n-6 linoleic acid; C18:3n-3 linolenic acid; C20:1<i>c</i>11 eicosenoic acid; C20:4n-6 arachidonic acid.</p

Unweighted principal component analysis.

<p>For: a) control and treatment animals in day 0 (blue), control animals in day 21 (green) and treatment animals in day 21 (red) b) control animals in day 0 (blue) and control animals in day 21 (red) c) treatment animals in day 0 (blue) and treatment animals in day 21(red) d) control animals in day 21 (blue) and treatment animals in day 21 (red). UPGMA clustering and Jackkniffing for the unweighted UniFrac data e) For the UPGMA cladogram on the left: Orange colour represents animals in day 0; red for the control animals in day 21 and blue for the treatment animals in day21. d) For the Jackknife supported tree layout the labels are coloured according to the group as: Black for animals in day 0; red for the control animals in day 21 and blue for treatment animals in day 21. The lines are coloured by the Jackknife supported percentages: Red for 75–100% support; Green for 50–75% support; Yellow for 25–50% support and Blue for <25% support.</p

Percentage relative abundance of OTUs observed at the phylum, class, order and family levels in the pig distal gut microbiota at day 0, day 21 C (control) and day 21 T (treatment-cider yeast supplemented) groups.

<p>Percentage relative abundance of OTUs observed at the phylum, class, order and family levels in the pig distal gut microbiota at day 0, day 21 C (control) and day 21 T (treatment-cider yeast supplemented) groups.</p

Primers used in this study.

<p>AG: aminoglycoside.</p><p>Primers used in this study.</p

MICs of gentamicin resistant clones.

<p>MICs of gentamicin resistant clones.</p

MICs of ampicillin resistant clones.

<p>MICs of ampicillin resistant clones.</p

Sequencing results from TOPO cloning of ampicillin and gentamicin resistant genes from metagenomic DNA.

<p>* Result presented is the closest homologue to our sequence based on lowest E value.</p><p>ESBL: extended spectrum β-lactamase.</p><p>AG: aminoglycoside.</p><p>Sequencing results from TOPO cloning of ampicillin and gentamicin resistant genes from metagenomic DNA.</p