5 research outputs found
Phylogenetic Trees of Six Representative Isolates Based on Comparison of 16S rDNA and Five Putative Virulence-Associated-Factor Genes with Known Sequences
<p>Swine isolates from Sichuan (
S. suis ZYS3 and
S. suis ZYS8) labeled in green, human isolates (
S. suis ZYH13 and
S. suis ZYH14) from Sichuan labeled in red, Jiangsu isolates from 1998 (
S. suis 9801 and
S. suis Habb) labeled in blue, and the standard highly virulent strain
S. suis P1/7 labeled in pink. All representative strains from other streptococcus species or isolates of
S. suis 2 are as indicated in the tree.
</p
Microscopic Characterization of Sectioned Liver Tissue from Patients Who Had Died
<div><p>(A) Light image of a liver tissue section (×100). The central vein is indicated with an arrow.</p>
<p>(B) Light image of a liver tissue section (×200).</p>
<p>(C) The convergent zone is indicated with an arrow (×100).</p>
<p>(D) TEM image of a liver tissue section (×20,000). A bacterium found in the tissue is highlighted with an arrow.</p></div
RFLP Analysis of Different S. suis 2 Isolates
<p>
S. suis S10: a highly virulent strain from China;
<i>S</i>.
<i>suis</i> 9801: swine isolate from Jiangsu Province in 1998;
S. suis Habb: human isolate from Jiangsu Province in 1998;
S. suis ZYS3: swine isolate from Sichuan Province in 2005;
S. suis ZYH13: human isolate from Sichuan Province in 2005; M: 1 kb DNA Ladder (MBI Ferments, Gdansk, Poland).
</p
Immunohistochemical Analysis of Liver Tissue Sections from Dead Patients Incubated with Normal and S. suis 2-Infected Swine Serum
<div><p>(A) Staining with normal swine serum detected no
S. suis 2 antigen.
</p>
<p>(B) Staining with the serum from the infected swine indicated the presence of
S. suis 2 antigen.
</p></div
Detection of the Pathogenic SS2 and Identification of Its Specific Genes
<div><p>(A) Light microscopy image of the isolates cultured from autopsy specimens.</p>
<p>GP<sup>+</sup> cocci (pointed to with black arrows) are arranged in various short chains (×100).
</p>
<p>(B) Qualitative PCR detection of isolates from the liver of fatal human cases with a set of primers specific for
<i>S</i>.
<i>suis</i> 2. M: 100bp DNA marker (Fermentas, Vilnius, Lithuania). CK: 16S rDNA PCR product from the R 735 standard strain of
S. suis 2. Multi-PCR: performed with a set of unique primers specific for
<i>mrp, epf, suilysin,</i> and
<i>cps-2J,</i> respectively.
</p></div