Mesoporous Silica and Composite Nanostructures for Theranostics

We discus methods for fabrication of silica and composite nanoparticles, which can be used in various biomedical applications. The most promising types of such nanostructures are hollow silica nanosheres, sil-ica coated plasmon-resonant nanoparticles (gold nanorods and gold-silver nanocages) and nanorattles. Mesoporous silica shell can be doped by desirable targeting molecules. Here we present the results of for-mation of nanocomposites composed of gold nanorods and double-layer silica shell. The secondary mesopo-rous silica shell is doped with a photosensitizer (hematoporphyrine in our case). We demonstate some of promising theranostics applications of these nanocomposites for bioimaging and in vivo therapy of tumors. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3548

Mesoporous Silica and Composite Nanostructures for Theranostics

We discus methods for fabrication of silica and composite nanoparticles, which can be used in various biomedical applications. The most promising types of such nanostructures are hollow silica nanosheres, sil-ica coated plasmon-resonant nanoparticles (gold nanorods and gold-silver nanocages) and nanorattles. Mesoporous silica shell can be doped by desirable targeting molecules. Here we present the results of for-mation of nanocomposites composed of gold nanorods and double-layer silica shell. The secondary mesopo-rous silica shell is doped with a photosensitizer (hematoporphyrine in our case). We demonstate some of promising theranostics applications of these nanocomposites for bioimaging and in vivo therapy of tumors. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3548

Адресная доставка фотосенсибилизатора для фотохимиотерапии витилиго

Aim. In this work, the authors set out to develop an effective method for the intrafollicular delivery of “Ammi majus fructuum furocumarines” photosensitizer (AMFF) followed by UVA irradiation (λ = 320–400 nm). Materials and methods. The proposed delivery method consists in using calcium carbonate particles acting as AMFF carriers. In vivo monitoring of hair follicle filling was carried out via optical coherence tomography, as well as by means of analyzing epilated hair using confocal laser scanning microscopy. Following the administration of free and encapsulated AMFF to three healthy volunteers, the character of UVA-induced skin pigmentation was registered under dermatoscopic examination. Results. The obtained results demonstrate a profuse filling of hair follicles with calcium carbonate particles, thus confirming the possibility of intrafollicular photosensitizer delivery. It was established that exposure to UVA irradiation causes intense pigment accumulation in the area of AMFF carrier administration. Conclusion. The proposed method of the targeted photosensitizer delivery allows photochemical therapy to be improved.Цель: разработка эффективного способа доставки фотосенсибилизатора Ammi majus fructuum furocumarines (AMFF) в волосяные фолликулы с последующим применением УФА-облучения (λ = 320–400 нм). Материалы и методы. Предлагаемый способ доставки заключается в применении частиц карбоната кальция, выступающих в роли контейнера-носителя AMFF. Исследование заполнения волосяных фолликулов было проведено in vivo методом оптической когерентной томографии, а также путем экстракции волос с последующим исследованием методом лазерной конфокальной флуоресцентной микроскопии. Регистрация характера пигментации кожи 3 здоровых добровольцев после сеанса УФА-облучения участков с предварительным внедрением препарата AMFF в свободном и иммобилизованном виде была осуществлена методом дерматоскопии. Результаты. Продемонстрировано обильное заполнение волосяных фолликулов частицами карбоната кальция, доказана возможность интрафолликулярной доставки фотосенсибилизатора с их помощью. Установлено, что в результате УФА-облучения в месте внедрения контейнеров, нагруженных AMFF, происходит значительное накопление пигмента. Заключение. Предложенный способ адресной доставки фотосенсибилизатора позволяет оптимизировать методику фотохимиотерапевтического воздействия

ОСТ investigation of influence of cement kiln dust and keratoprotectoral medication on the cornea structure.

The research goal: in vitro study of cornea permeability at the action of cement kiln dust and keratoprotectoral medication. Materials and Methods. Experimental studies were carried out with twenty eyes often rabbits. The measurements were performed using ОСТ system OCP930SR 022 (Thorlabs, USA). Results. ОСТ tomography was used to monitor the attenuation coefficient of the cornea in vitro during the permeability of cement kiln dust and keratoprotector Systein Ultra. The permeability coefficients of the cornea for water, cement dust and keratoprotector were measured. A computer model was developed that allowed one to analyze the diffusion of these substances in the eye cornea. Conclusion. It is shown: 1) cement dust falling on the eye cornea caused pronounced dehydration of the tissue (thickness decreasing) and led to the increase in the coefficient of light attenuation, which in the conditions of cement production could affect the deterioration of the eyesight of workers; 2) the application of the keratoprotector on the eye cornea when exposed by cement dust, slowed significantly the dehydration process and did not cause the increase in the light attenuation coefficient, which characterized the stabilization of visual functions in cement production workers. In this case, the keratoprotector itself did not cause dehydration and led to the decrease in the light attenuation coefficient, which could allow it to be used for a long time in the order to protect the organ of vision from the negative effects of cement dust.</p

Analysing coupling architecture in the cortical EEG of a patient with unilateral cerebral palsy

The detection of coupling presence and direction between cortical areas from the EEG is a popular approach in neuroscience. Granger causality method is promising for this task, since it allows to operate with short time series and to detect nonlinear coupling or coupling between nonlinear systems. In this study EEG multichannel data from adolescent children, suffering from unilateral cerebral palsy were investigated. Signals, obtained in rest and during motor activity of affected and less affected hand, were analysed. The changes in inter-hemispheric and intra-hemispheric interactions were studied over time with an interval of two months. The obtained results of coupling were tested for significance using surrogate times series. In the present proceeding paper we report the data of one patient. The modified nonlinear Granger causality is indeed able to reveal couplings within the human brain

Evaluation of nonlinear properties of epileptic activity using largest Lyapunov exponent

Absence seizures are known to be highly non-linear large amplitude oscillations with a well pronounced main time scale. Whilst the appearance of the main frequency is usually considered as a transition from noisy complex dynamics of baseline EEG to more regular absence activity, the dynamical properties of this type of epileptiformic activity in genetic absence models was not studied precisely. Here, the estimation of the largest Lyapunov exponent from intracranial EEGs of 10 WAG/Rij rats (genetic model of absence epilepsy) was performed. Fragments of 10 seizures and 10 episodes of on-going EEG each of 4 s length were used for each animal, 3 cortical and 2 thalamic channels were analysed. The method adapted for short noisy data was implemented. The positive values of the largest Lyapunov exponent were found as for baseline as for spike wave discharges (SWDs), with values for SWDs being significantly less than for on-going activity. Current findings may indicate that SWD is a chaotic process with a well pronounced main timescale rather than a periodic regime. Also, the absence activity was shown to be less chaotic than the baseline one. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only