Toxicologic screening of fungi isolated from millet (pennisetum spp) during the rainy and dry harmattan seasons in Niger state, Nigeria

A survey of fungi contaminating mouldy field, stored and marketed millet samples collected during rainy and dry harmattan seasons of the year 2000 from the twenty five local government areas of NigerState, Nigeria, was conducted. Some of the fungal isolates from the two groups of samples were screened for their mycotoxin producing potentials in mice. Aflatoxin B1 content of the rainy season millet samples was determined. Aspergillus niger was the predominant fungi found in millet during the rains. Twelve out of the 49 wet season millet samples were contaminated with AFB1 at concentrations between 1370.28 and 3495.10 ug/kg. Penicillium spp. was the commonest contaminant of millet during the dry harmattan season. Thirty five of the fifty five fungal isolates screened for toxicity were found to produce toxic metabolites that were lethal to mice and were isolates of Aspergillus, Fusarium, Penicillium, Rhizopus, Mucor, Syncephalastrum, and Helminthosporium

Fungi and some mycotoxins contaminating rice (Oryza Sativa) in Niger State, Nigeria

Study on the fungi and some mycotoxins (Aflatoxin B1 - AFB1, ochratoxin A - OTA and zearalenone -ZEN) contaminating rice (Oryza sativa) in Niger State of Nigeria was carried out. One thousand and sixty two fungi were isolated and identified from one hundred and ninety six mouldy rice samples collected from the state. The major fungal genera contaminating rice were Aspergillus, Penicillium, Fusarium,Alternaria, Mucor, Rhizopus, Trichoderma, Curvularia,  elminthosporium and Cladosporium. The most prevalent fungal species on rice were .Penicillium spp., A. flavus, A. parasiticus, A. niger, Mucor spp.,Rhizopus spp. and Alternaria spp. The commonest fungal contaminants of the dry, harmattan; dry-hot and rainy seasons were A. niger, Penicillium spp. and A. flavus respectively. AFB1 was detected in 97 ofthe samples analyzed at concentrations between 20-1642 ug/kg. Fifty six of the one hundred and forty samples analyzed for OTA contained the toxin (24 – 1164 ug/kg). Zearalenone was found in ninety threeof the one hundred and ninety six mouldy rice samples analyzed at concentrations of between 24 and 1169 ug/kg. Twenty two samples were concurrently contaminated with the three toxins while sevenothers were found to contain both AFB1 and OTA. AFB1 and ZEN occurred together in twelve samples, and eight samples contained both OTA and ZEN

Anti-Trypanosomal Potential Of Momordica Balsamina Linn Fruit Pulp Extract Against Trypanosoma brucei brucei Infection

The search for new trypanocides has not been keenly pursued due to high cost of design and development with no promise of financial returns. Momordica balsamina fruit pulp extract was screened for antitrypanosomal activity in experimental Trypanosoma brucei brucei infection in rabbits. The extract was administered prior to parasite inoculation, 24 hours post parasite inoculation and on establishment of infection. The treatment was by oral administration of the extract at 500mg/kg body weight for 14 consecutive days. Parasitaemia was monitored daily while body weight and packed cell volume (PCV) were determined before commencement of studies and subsequently at weekly intervals for 28 days. The result showed a significant (