CHOICE OF TECHNOLOGY: THE CASE OF GRASS FED VERSUS GRAIN FED CATTLE IN HAWAII

A model describing the choice of technology is developed from theoretical considerations. It is shown that the model can be approximated using a logit function. Estimates of short-run elasticities are easily obtained. The model is then applied to the decision to place feeder cattle in confined feeding situations or on range. With one possible exception, the results are consistent with theoretical expectations and with previous studies.Livestock Production/Industries,

DEMAND FOR PLANT NUTRIENTS IN TENNESSEE DISAGGREGATED BY MIXED FERTILIZERS AND DIRECT APPLICATION MATERIALS

When obtaining nitrogen (N), phosphate (P) and potash (K), purchasing decisions concerning the quantity and the form of each plant nutrient must be made. Logit models are estimated for the choice-of-form decision by considering those variables influencing the probability that plant nutrients will be purchased in Tennessee as part of a mixed fertilizer or as direct application materials. Parameter and elasticity estimates can be used by fertilizer manufacturers and distributors to anticipate changes in the composition of demand for plant nutrients in Tennessee.Crop Production/Industries,

AN ANALYSIS OF THE USE OF GRADES AND HOUSEBRAND LABELS IN THE RETAIL BEEF MARKET

The congruence of beef consumersÂ’ purchases with their stated preferences regarding internal fat content are examined. The role of U.S. Department of Agriculture (USDA) grades and housebrand labeling of beef in providing information to consumers is studied in the theoretical framework of search theory. The empirical results indicate that the current system of USDA grades and housebrand labels is not disseminating information regarding internal fat content effectively to consumers. Suggestions are made for providing consumers with better information and education necessary to increase congruence of expressed preferences regarding internal fat content and actual beef purchases.Food Consumption/Nutrition/Food Safety, Livestock Production/Industries,

Stroma Regulates Increased Epithelial Lateral Cell Adhesion in 3D Culture: A Role for Actin/Cadherin Dynamics

Cell shape and tissue architecture are controlled by changes to junctional proteins and the cytoskeleton. How tissues control the dynamics of adhesion and cytoskeletal tension is unclear. We have studied epithelial tissue architecture using 3D culture models and found that adult primary prostate epithelial cells grow into hollow acinus-like spheroids. Importantly, when co-cultured with stroma the epithelia show increased lateral cell adhesions. To investigate this mechanism further we aimed to: identify a cell line model to allow repeatable and robust experiments; determine whether or not epithelial adhesion molecules were affected by stromal culture; and determine which stromal signalling molecules may influence cell adhesion in 3D epithelial cell cultures.The prostate cell line, BPH-1, showed increased lateral cell adhesion in response to stroma, when grown as 3D spheroids. Electron microscopy showed that 9.4% of lateral membranes were within 20 nm of each other and that this increased to 54% in the presence of stroma, after 7 days in culture. Stromal signalling did not influence E-cadherin or desmosome RNA or protein expression, but increased E-cadherin/actin co-localisation on the basolateral membranes, and decreased paracellular permeability. Microarray analysis identified several growth factors and pathways that were differentially expressed in stroma in response to 3D epithelial culture. The upregulated growth factors TGFβ2, CXCL12 and FGF10 were selected for further analysis because of previous associations with morphology. Small molecule inhibition of TGFβ2 signalling but not of CXCL12 and FGF10 signalling led to a decrease in actin and E-cadherin co-localisation and increased paracellular permeability.In 3D culture models, paracrine stromal signals increase epithelial cell adhesion via adhesion/cytoskeleton interactions and TGFβ2-dependent mechanisms may play a key role. These findings indicate a role for stroma in maintaining adult epithelial tissue morphology and integrity