2 research outputs found
In-depth proteomic characterization of classical and non-classical monocyte subsets
Monocytes are bone marrow-derived leukocytes that are part of the innate immune
system. Monocytes are divided into three subsets: classical, intermediate and non-classical,
which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16.
These cells are key players in the inflammation process underlying the mechanism of many
diseases. Thus, the molecular characterization of these cells may provide very useful information
for understanding their biology in health and disease. We performed a multicentric proteomic
study with pure classical and non-classical populations derived from 12 healthy donors. The robust
workflow used provided reproducible results among the five participating laboratories. Over 5000
proteins were identified, and about half of them were quantified using a spectral counting approach.
The results represent the protein abundance catalogue of pure classical and enriched non-classical
blood peripheral monocytes, and could serve as a reference dataset of the healthy population.
The functional analysis of the differences between cell subsets supports the consensus roles assigned
to human monocytes
In-depth proteomic characterization of classical and non-classical monocyte subsets
Monocytes are bone marrow-derived leukocytes that are part of the innate immune
system. Monocytes are divided into three subsets: classical, intermediate and non-classical,
which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16.
These cells are key players in the inflammation process underlying the mechanism of many
diseases. Thus, the molecular characterization of these cells may provide very useful information
for understanding their biology in health and disease. We performed a multicentric proteomic
study with pure classical and non-classical populations derived from 12 healthy donors. The robust
workflow used provided reproducible results among the five participating laboratories. Over 5000
proteins were identified, and about half of them were quantified using a spectral counting approach.
The results represent the protein abundance catalogue of pure classical and enriched non-classical
blood peripheral monocytes, and could serve as a reference dataset of the healthy population.
The functional analysis of the differences between cell subsets supports the consensus roles assigned
to human monocytes