158 research outputs found
Mammalian cells in culture actively export specific microRNAs
The discovery of microRNAs (miRNAs) as a new class of regulators of gene expression has triggered an explosion of research, but has left many unanswered questions about how this regulation works and how it is integrated with other regulatory mechanisms. A number of miRNAs have been found to be present in blood plasma and other body fluids of humans and mice in surprisingly high concentrations. This observation was unexpected in two respects: first, the fact that these molecules are present at all outside the cell at significant concentrations; and second, that these molecules appear to be stable outside of the cell. In light of this it has been suggested that the biological function of miRNAs may also extend outside of the cell and mediate cell-cell communication^[1-5]^. Such a system would be expected to export specific miRNAs from cells in response to specific biological stimuli. We report here that after serum deprivation several human cell lines tested do export a spectrum of miRNAs into the culture medium. The export response is substantial and prompt. The exported miRNAs are found both within and outside of microvesicles and exosomes. We have identified some candidate protein components of this system outside the cell, and found one exported protein that plays a role in protecting miRNA from degradation. Our results point to a hitherto unrecognized and uncharacterized miRNA trafficking system in mammalian cells that may involve cell-cell communication
Describing the complexity of systems: multi-variable "set complexity" and the information basis of systems biology
Context dependence is central to the description of complexity. Keying on the
pairwise definition of "set complexity" we use an information theory approach
to formulate general measures of systems complexity. We examine the properties
of multi-variable dependency starting with the concept of interaction
information. We then present a new measure for unbiased detection of
multi-variable dependency, "differential interaction information." This
quantity for two variables reduces to the pairwise "set complexity" previously
proposed as a context-dependent measure of information in biological systems.
We generalize it here to an arbitrary number of variables. Critical limiting
properties of the "differential interaction information" are key to the
generalization. This measure extends previous ideas about biological
information and provides a more sophisticated basis for study of complexity.
The properties of "differential interaction information" also suggest new
approaches to data analysis. Given a data set of system measurements
differential interaction information can provide a measure of collective
dependence, which can be represented in hypergraphs describing complex system
interaction patterns. We investigate this kind of analysis using simulated data
sets. The conjoining of a generalized set complexity measure, multi-variable
dependency analysis, and hypergraphs is our central result. While our focus is
on complex biological systems, our results are applicable to any complex
system.Comment: 44 pages, 12 figures; made revisions after peer revie
Comparison of reproducibility, accuracy, sensitivity, and specificity of miRNA quantification platforms
Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for microRNA (miRNA) quantification in biofluids is of high priority. We compare four platforms for these characteristics: small RNA sequencing (RNA-seq), FirePlex, EdgeSeq, and nCounter. For a pool of synthetic miRNAs, coefficients of variation for technical replicates are lower for EdgeSeq (6.9%) and RNA-seq (8.2%) than for FirePlex (22.4%); nCounter replicates are not performed. Receiver operating characteristic analysis for distinguishing present versus absent miRNAs shows small RNA-seq (area under curve 0.99) is superior to EdgeSeq (0.97), nCounter (0.94), and FirePlex (0.81). Expected differences in expression of placenta-associated miRNAs in plasma from pregnant and non-pregnant women are observed with RNA-seq and EdgeSeq, but not FirePlex or nCounter. These results indicate that differences in performance among miRNA profiling platforms impact ability to detect biological differences among samples and thus their relative utility for research and clinical use
An integrative approach to inferring biologically meaningful gene modules
<p>Abstract</p> <p>Background</p> <p>The ability to construct biologically meaningful gene networks and modules is critical for contemporary systems biology. Though recent studies have demonstrated the power of using gene modules to shed light on the functioning of complex biological systems, most modules in these networks have shown little association with meaningful biological function. We have devised a method which directly incorporates gene ontology (GO) annotation in construction of gene modules in order to gain better functional association.</p> <p>Results</p> <p>We have devised a method, Semantic Similarity-Integrated approach for Modularization (SSIM) that integrates various gene-gene pairwise similarity values, including information obtained from gene expression, protein-protein interactions and GO annotations, in the construction of modules using affinity propagation clustering. We demonstrated the performance of the proposed method using data from two complex biological responses: 1. the osmotic shock response in <it>Saccharomyces cerevisiae</it>, and 2. the prion-induced pathogenic mouse model. In comparison with two previously reported algorithms, modules identified by SSIM showed significantly stronger association with biological functions.</p> <p>Conclusions</p> <p>The incorporation of semantic similarity based on GO annotation with gene expression and protein-protein interaction data can greatly enhance the functional relevance of inferred gene modules. In addition, the SSIM approach can also reveal the hierarchical structure of gene modules to gain a broader functional view of the biological system. Hence, the proposed method can facilitate comprehensive and in-depth analysis of high throughput experimental data at the gene network level.</p
Duplication Models for Biological Networks
Are biological networks different from other large complex networks? Both
large biological and non-biological networks exhibit power-law graphs (number
of nodes with degree k, N(k) ~ k-b) yet the exponents, b, fall into different
ranges. This may be because duplication of the information in the genome is a
dominant evolutionary force in shaping biological networks (like gene
regulatory networks and protein-protein interaction networks), and is
fundamentally different from the mechanisms thought to dominate the growth of
most non-biological networks (such as the internet [1-4]). The preferential
choice models non-biological networks like web graphs can only produce
power-law graphs with exponents greater than 2 [1-4,8]. We use combinatorial
probabilistic methods to examine the evolution of graphs by duplication
processes and derive exact analytical relationships between the exponent of the
power law and the parameters of the model. Both full duplication of nodes (with
all their connections) as well as partial duplication (with only some
connections) are analyzed. We demonstrate that partial duplication can produce
power-law graphs with exponents less than 2, consistent with current data on
biological networks. The power-law exponent for large graphs depends only on
the growth process, not on the starting graph
- …