496 research outputs found
Procedures and operating instructions for diagnosis in vascular anomalies and pathology
In the last 30 years a revolution has occurred in the diagnosis and management of vascular anomalies. The great changes began with Mulliken and Glowacki separation of hemangiomas and vascular anomalies. Their work has now morphed into the ISSVA classification. Subsequently the discovery of the significance of the presence of GLUT-1 in the diagnosis of the hemangiomas of infancy gave us a new marker in our quest for accurate classification. Now genetic breakthroughs have led us into a \u201cStar Wars\u201d like environment in the experimental laboratory. During all these events the critical role of the pathologist has become more evident. Understanding the histopathology of anomalies has greatly aided in our approach to therapies. Moreover, genetic findings do not have full significance without the morphologic framework
Supracondylar process of the humerus in children. Cases from the 15th- 18th century in Settimo Vittone (Piedmont).
procedures and operating instructions for diagnosis in vascular anomalies and pathology
In the last 30 years a revolution has occurred in the diagnosis and management of vascular anomalies. The great changes began with Mullikenand Glowacki separation of hemangiomas and vascular anomalies. Their work has now morphed into the ISSVA classification. Subsequentlythe discovery of the significance of the presence of GLUT-1 in the diagnosis of the hemangiomas of infancy gave us a new markerin our quest for accurate classification. Now genetic breakthroughs have led us into a "Star Wars" like environment in the experimental laboratory.During all these events the critical role of the pathologist has become more evident. Understanding the histopathology of anomalieshas greatly aided in our approach to therapies. Moreover, genetic findings do not have full significance without the morphologic framewor
Proteomic profiling of human amnion for preterm birth biomarker discovery
Spontaneous preterm birth (PTB) complicates about 12% of pregnancies worldwide, remaining the
main cause of neonatal morbidity and mortality. Spontaneous preterm birth PTBs is often caused by
microbial-induced preterm labor, mediated by an inflammatory process threatening both maternal
and newborn health. In search for novel predictive biomarkers of PTB and preterm prelabor rupture
of the membranes (pPROM), and to improve understanding of infection related PTB, we performed
an untargeted mass spectrometry discovery study on 51 bioptic mid zone amnion samples from
premature babies. A total of 6352 proteins were identified. Bioinformatics analyses revealed a ranked core of 159 proteins maximizing the discrimination between the selected clinical stratification groups allowing to distinguish conditions of absent (FIR 0) from maximal Fetal Inflammatory Response (FIR 3) stratified in function of Maternal Inflammatory Response (MIR) grade. Matrix metallopeptidase-9 (MMP-9) was the top differentially expressed protein. Gene Ontology enrichment analysis of the core proteins showed significant changes in the biological pathways associated to inflammation and regulation of immune and infection response. Data suggest that the conditions determining PTB would be a transversal event, secondary to the maternal inflammatory response causing a breakdown in fetal-maternal tolerance, with fetal inflammation being more severe than maternal one. We also highlight matrix metallopeptidase-9 as a potential predictive biomarker of PTB that can be assayed in the maternal serum, for future investigation
Loss of Wwox Perturbs Neuronal Migration and Impairs Early Cortical Development
Mutations in the WWOX gene cause a broad range of ultra-rare neurodevelopmental and brain degenerative disorders, associated with a high likelihood of premature death in animal models as well as in humans. The encoded Wwox protein is a WW domain-containing oxidoreductase that participates in crucial biological processes including tumor suppression, cell growth/differentiation and regulation of steroid metabolism, while its role in neural development is less understood. We analyzed the exomes of a family affected with multiple pre- and postnatal anomalies, including cerebellar vermis hypoplasia, severe neurodevelopmental impairment and refractory epilepsy, and identified a segregating homozygous WWOX mutation leading to a premature stop codon. Abnormal cerebral cortex development due to a defective architecture of granular and molecular cell layers was found in the developing brain of a WWOX-deficient human fetus from this family. A similar disorganization of cortical layers was identified in lde/lde rats (carrying a homozygous truncating mutation which disrupts the active Wwox C-terminal domain) investigated at perinatal stages. Transcriptomic analyses of Wwox-depleted human neural progenitor cells showed an impaired expression of a number of neuronal migration-related genes encoding for tubulins, kinesins and associated proteins. These findings indicate that loss of Wwox may affect different cytoskeleton components and alter prenatal cortical development, highlighting a regulatory role of the WWOX gene in migrating neurons across different species
2D Axisymmetric Coupled Computational Fluid Dynamics–Kinetics Modeling of a Nonthermal Arc Plasma Torch for Diesel Fuel Reforming
Importance of osteologic collections in museums of pathologic anatomy as materials for study and comparison in paleopathology [Importanza delle raccolte osteologiche nei Musei di anatomia patologica quali materiali di studio e di confronto per la paleopatologia.]
- …