Correlation between miRNA-24 expression and TRIB3 mRNA and protein expression.

a<p>Spearman's rho.</p

Associations of <i>miRNA-24</i> expression levels with clinicopathological characteristics.

a<p>Due to missing values, numbers do not always add up to 94.</p>b<p><i>P</i> tested with Mann-Whitney U test or Kruskal-Wallis Test where appropriate.</p

Effect of siRNA mediated knockdown of TRIB3 on mRNA and protein levels.

<p>A. Fold induction of TRIB3 mRNA in MDA-MB-231 cells during normoxia and after 24 hours of hypoxia (1% oxygen) treated with negative control siRNA (SCR) or siRNA mediated TRIB3 knockdown (TRIB3-KD) (n = 8). B. Fold induction of TRIB3 protein intensity in MDA-MB-231 cells during normoxia or after 24 hours of hypoxia (1% oxygen) treated with negative control siRNA (SCR) or siRNA mediated TRIB3 knockdown (TRIB3-KD) (n = 3). Protein intensities were controlled for α-tubulin intensities on same blot. Fold induction are averages (+ SD) relative to levels in cells treated with control siRNA (SCR) during normoxia.</p

Effect of proteasome inhibition on TRIB3 protein levels.

<p>A. Western blot of MDA-MB-231 cells treated with MG132 and exposed to hypoxia (0.1% oxygen) immunostained for β-catenin (positive MG132 control), TRIB3 and α-tubulin (loading control). B. MDA-MB-231 cells treated with hydroxychloroquine (CQ) and exposed to hypoxia (1% oxygen) immunostained for LC3b (positive autophagy inhibition marker), TRIB3 and α-tubulin (loading control). C. MDA-MB-231 cells treated with cycloheximide (CHX) and exposed to hypoxia (1% oxygen) immunostained for HIF-1 (positive translation inhibition control), TRIB3 and α-tubulin (loading control). D. MCF-7 cells treated with cycloheximide (CHX) and exposed to hypoxia (1% oxygen) immunostained for HIF-1 (positive translation inhibition control), TRIB3 and α-tubulin (loading control).</p

TRIB3 mRNA translation during anoxia.

<p>A. Polysome profiles of MCF7 cells grown under aerobic of anoxic conditions during 24 hours. B. TRIB3 mRNA levels determined by Q-PCR in the total RNA fraction. C. TRIB3 mRNA distribution within the polysome. D. Change in translation efficiency of the TRIB3 mRNA after exposure to anoxia for 24 hrs. Experiments were performed in triplicates.</p

Breast cancer patient survival based on miRNA-24 expression levels.

<p>A. Disease free survival in patients stratified according to miRNA-24 expression below or above median. B. Overall survival in patients stratified according to miRNA-24 expression below or above median (0.9×10⁻³). C. Disease free survival in patients stratified according to tertiles miRNA-24 expression below 0.5×10⁻³, above 1.2×10⁻³ or in between. D. Overall survival in patients stratified according to tertiles miRNA-24 expression below 0.5×10⁻³, above 1.2×10⁻³ or in between.</p

miRNA 24 expression levels in groups with accordance and discordance between TRIB3 mRNA and protein.

<p>miRNA 24 expression levels in groups with accordance and discordance between TRIB3 mRNA and protein.</p

Circulating cardiovascular risk markers in adult CAH patients and healthy, matched controls.

<p>Mean values±1 SD are given.</p><p>*As hsCRP values were not distributed normally, we have calculated log(CRP) values.</p

Lipid profile in adult CAH patients and healthy, matched controls.

<p>Mean values±1 SD are given.</p

Mean 24-hour ambulatory blood pressure measurements (±1 SD) in CAH patients (open squares) and matched controls (closed squares).

<p>Mean 24-hour ambulatory blood pressure measurements (±1 SD) in CAH patients (open squares) and matched controls (closed squares).</p