Evolution of Salmonella excretion by sows during gestation in link with the faecal microbiome

Pork meat is estimated to be responsible of 10 to 20% of human salmonellosis cases. Control strategies at the farm could reduce contamination at the slaughterhouse. One of the targeted sector of the production is the maternity, where sows could be Salmonella reservoirs. The aim of this study was to characterize the faecal microbiome of sows excreting or not Salmonella during gestation phases. A total of 76 sows were selected and fecal matters were analysed at the beginning or the end of gestation period. Salmonella detection was conducted using a method including two selective enrichment media (MSRV and TBG). Nine (9) isolates per positive samples were collected. Among the 76 sows tested, 31 were shedding Salmonella. The sows in the first third of their gestation shed significantly more frequently Salmonella (22/29) than those in the last third (9/47) (χ² P \u3c 0.05). The shedding status of 19 of the sows that were previously sampled in the first third of their gestation was followed, this time in the last third, confirming reduction of the shedding. Association between changes in the intestinal microbiome and this evolution of Salmonella shedding will be explored. MiSeq sequencing is currently being conducted on the feces to identify shifts in the composition or diversity in the microbial community that could be associated to these variations. A large number of Salmonella isolates that were collected were genotyped by a high resolution melt (HRM) technique. These results showed the presence of a major HRM profile (136 isolates / 169) and two minor profiles (24 and 9 /169)

Survival in water of Campylobacter jejuni strains isolated from the slaughterhouse

Campylobacter jejuni cause gastroenteritis in humans. The main transmission vector is the consumption or handling of contaminated chicken meat, since chicken can be colonized asymptomatically by C. jejuni. However, water has been implicated as the transmission vector in a few outbreaks. One possibility is the contamination of water effluent by C. jejuni originating from chicken farm. The ability of C. jejuni to be transmitted by water would be closely associated to its ability to survive in water. Therefore, in this study, we have evaluated the ability of reference strains and chickenisolated strains to survive in water. Defined water media were used, since the composition of tap water is variable. We showed that some isolates survive better than others in defined freshwater (Fraquil) and that the survival was affected by temperature and the concentration of NaCl. By comparing the ability of C. jejuni to survive in water with other phenotypic properties previously tested, we showed that the ability to survive in water was negatively correlated with autoagglutination. Our data showed that not all chicken isolates have the same ability to survive in water, which is probably due to difference in genetic content

Digestive microbiota changes during application of an effective, feed presentation based, mitigation option against Salmonella shedding in pigs

If some studies have attempted to mitigate the Salmonella spp. excretion in pigs by feed related interventions, none clearly demonstrated the impact of the presentation (mash or pellet and particular size). Thus this study aimed to determine if the modification of the pigs feed presentation alone can lower the Salmonella spp. excretion

Residual contamination detection and serovar distribution of Listeria monocytogenes isolates in pork slaughterhouse and cutting facilities in province of Quebec

L. monocytogenes (L. mono) is recognised as a zoonotic foodborne pathogen. Its control is focused on the “Readyto Eat” food production level. Recently, Health Canada had reinforced its “Policy on L. mono in Ready-to-Eat Foods”, highlighting environmental surveillance and control of meat processing facilities as important risk reduction tools. The industry wants to improve its management of L. mono risk, taking into account previous steps of meat production. Nowadays, few information are available on the presence, distribution and types of strains in the environments concerning this pathogen in pork slaughterhouses and cutting facilities in Canada. Our objective was to detect and described residual L. mono contamination and analyse serovars distribution in different areas in the pork production continuum in and between slaughterhouses and cutting facilities in province of Quebec, during a one year period. Such data are a pre-requisite to achieve the optimization of the management measures by the industrials

Seasonal ability of biofilm formation of Listeria monocytogenes strains isolated from slaughterhouses in Quebec

Listeria monocytogenes is a pathogenic bacterium found in the environment and food. It causes Listeriosis, a serious disease that presents largest fatality rate among foodborne illness. The persistence of these bacteria in food processing plants, favoured by biofilm formation is a major problem both for industry and public health. The aim of the study was to compare strains isolated from three sectors in slaughterhouses for their ability to form biofilms. The strains were isolated from holding pens, slaughtering, and cutting in 4 slaughterhouses and for 3 different periods of the year. Biofilm formation analyses were performed for 186 strains under static conditions at 30 °C for 48 hours and 7 °C for 15 days, frequent temperature in meat processing plants. Biomass production in BHI is measured in microplates after staining with crystal violet and measuring the absorbance at 595 nm, the data is normalized to obtain inter strain comparability in 96 well plate. Results show significant difference in biofilm formation between strains according to the period of sampling and origin of strains (slaughterhouse). Biofilm formation by strains belonging to the sampling of period 2 (February to May 2014) were greater than in summer 2015 at 30 °C (p\u3c0,0001) and at 7°C (p=0,019). Strains isolated from plants 1 and 2 produced more biomass than those collected from plant 4 at 30°C (p=0,0015) and 7°C, biofilm formation in isolates from plants 1and 2 were greater than in strains from plants 3 and 4 (p\u3c0,0001). No difference in biofilm formation ability was revealed depending on the place sampled in the plant (holding pens, slaughter or cutting rooms), whatever the temperature tested. Among strains studied here, strong differences in biofilm forming ability were identified; these results are the beginning of our in vestigation aiming to answer the question of strain persistence

Molecular serotyping and virulence potential of Listeria monocytogenes isolated from bovine, swine and human in the province of Quebec

Listeria monocytogenes (L mono) cause rare but critical diseases, particularly for at risk population that include pregnant women. Food-borne origin of listeriosis is clearly recognised only since 1984. Since then, a great number of grouped cases occurred and milk or meat products, particularly pork meat, were implicated. Management of this zoonotic pathogen considers all strains as at equal risk. Recently a new perspective for characterisation of strain virulence was allowed since unaltered sequence of InlA was recognised as a key for strain virulence

The use of a multifactorial approach to reduce Salmonella shedding

An analytic epidemiological survey was carried out in france to assess the circumstances for Salmonella shedding in finishing pigs. This study gave out a combination of risk factors. The aim of our study was to evaluate that the implementation of a programme based on these risk factors reduces Salmonella carriage

Effect of fumonisins and Salmonella on digestive flora profiles assessed using a molecular tool (CE-SSCP).

Fumonisins (FB) are mycotoxins frequently found in vegetal feedstuffs, especially in maize used for pig feeding. Among fumonisins, FB1 was the better described toxin. It caused pulmonary and hepatic damages as well as immune response disorders in pigs that were recognised as especially sensitive to FB Intoxication. The FB1 immunosuppressor induced a higher susceptibility of pigs to gut pathogens such as E coli. Effects on Salmonella have poorly been studied despite the frequent asymptomatic carnage in pigs and the presumptive role of nora equilibrium on prevention of Salmonella excretion or re-excretion. To determine the influence of Salmonella carriage, fumonisins or both on digestive flora equilibrium, the use of a molecular technique CE-SSCP (Capillary-Electrophoresis Single Strand Conformation Polymorphism) appeared a good complement to the conventional bacteriological techniques. The objective was to assess the perturbation of nora associated with co-exposition in experimental conditions in absence of clinical sign

Impact of mash feeding versus pellets on propionic/butyric acid levels and on total Escherichia coli load in the gastrointestinal tract of growing pigs

Feed characteristics may influence the bacterial community composition and metabolic activities in the pig gastrointestinal tract, known to be associated with positive effects on the gut. Use of mash feed is associated with reduced Salmonella excretion, but little is known of its effect on the Escherichia coli population or of the mechanism of action. Our objectives were to assess the effect of feed texture combined with feed particle size on VFA profiles and levels, total E. coli count, and the presence of genes encoding virulence factors of pathogenic E. coli strains in the digestive tract along with their impact on pig performance of fattening pigs. Pigs (n = 840) on a commercial farm received mash or pellet diets of different particle sizes during the fattening period. Caecal and colon contents from 164 pigs were sampled at the slaughterhouse for enumeration of E. coli by quantitative PCR (qPCR) and for VFA quantification by capillary gas chromatography. The yccT gene was used to enumerate total E. coli. Improved pig performances associated with pellet texture and a 500-μm size were observed. Caecal (P = 0.02) and colon (P < 0.01) propionic acid concentrations were lower for pigs receiving pellet rather than mash feed. Similarly, caecal (P = 0.01) and colon (P < 0.001) butyric acid concentrations were also lower for pigs receiving pellet rather than mash feed, as determined by capillary gas chromatography. Moreover, caecal (P = 0.03) and colon (P < 0.001) butyric acid concentrations were higher for pigs receiving a feed with a 1,250-μm particle size rather than a 500-μm particle size. On the other hand, total caecal and colon E. coli levels were higher for pigs receiving pellet feed than for those receiving mash feed. For total E. coli enumeration, caecal (P < 0.01) and colon (P < 0.01) yccT gene copies were higher for pigs receiving pellet rather than mash feed. No effect of particle size on fatty acid concentrations or on E. coli numbers was observed. Virulence gene quantification revealed no trend. Taken together, results showed that mash feed is associated with lower growth performance but with favorable intestinal changes linked to VFA levels and E. coli reduction in the intestine