<i>In vivo</i> morphological analysis and retinal layering with cSLO angiography and SD-OCT imaging.

<p>(<b>A–C</b>) Gerbillus perpallidus, (<b>D–F</b>) Meriones unguiculatus, and (<b>G–I</b>) Phodopus campbelli. cSLO en-face imaging of the vascular pattern using (<b>A</b>, <b>D</b>, <b>G</b>) ICG angiography mode (795 nm with barrier filter at 800 nm) depicts both retinal and choroidal structures. Black line depicts OCT scan direction. (<b>B</b>, <b>E</b>, <b>H</b>) FLA displays large retinal vessels and capillaries. Besides, choroidal vessels are visible in the visual steak region as different retinal layering allows light at λ = 488 nm to better penetrate the RPE/choriocapillary complex. (<b>C</b>, <b>F</b>, <b>I</b>) In vivo cross-sectional SD-OCT images show an increased retinal thickness in the visual streak region, indicating a specialized retinal region (arrows). ONL, outer nuclear layer; RPE, retinal pigment epithilium.</p

<i>In vivo</i> fundus imaging.

<p>cSLO fundus images of (<b>A</b>) human, (<b>B</b>) mouse, and (<b>C</b>) Meriones unguiculatus. (<b>A–C</b>) D = dorsal, V = ventral. (<b>A</b>) Native infrared (λ = 830 nm) SLO image of one researcher's right eye illustrates the sparse vascularization of the macular region. (<b>B</b>) Fluorescein angiography (FLA) (488 nm, barrier filter at 500 nm) reveals the evenly distributed retinal blood vessels in mice. (<b>C</b>) FLA image of Meriones unguiculatus visualizes the horizontal sparse vascular band denoting the specialized retinal region dorsal to the optic disc.</p

Detailed analysis of the retinal blood vessel distribution <i>in vivo</i> using cSLO and <i>in vitro</i> using collagen IV staining.

<p>(<b>A–C</b>) G. perpallidus, (<b>D–F</b>) M. unguiculatus, and (<b>G–I</b>) P. campbelli. Collagen IV stained retinal wholemounts (<b>A</b>, <b>D</b>, <b>G</b>) illustrate the entire visual streak region. Magnifications of wholemounts (<b>B</b>, <b>E</b>, <b>H</b>) and corresponding in vivo cSLO data (<b>C</b>, <b>F</b>, <b>I</b>) show that retinal vessels ramify and narrow into a sparse capillary net at the border of the respective area.</p

Immunofluorescence labeling of retinal sections revealed distinct differences in cone cell distribution and vessel density.

<p>(<b>A–F</b>) Areas within the visual streak region are shown in the right panel, areas from dorsal peripheral retina in the left panel. (<b>A</b>) Isolectin IB4-FITC staining of G. perpallidus retina reveals the sparse vessel distribution within the streak region; (<b>B</b>) Peanut agglutinin staining of P. campbelli retina illustrates increased cone densities within the streak region. (<b>C</b>) SWS cone staining of M. unguiculatus retina reveals enhanced SWS cone density within the streak area compared to dorsal periphery. Rods and MWS cones are evenly distributed across the retina as shown by (<b>D</b>) rod transducin (GNAT1) staining in M. unguiculatus, (<b>E</b>) rod opsin staining, and (F) MWS cone opsin staining in G. perpallidus. (<b>A–F</b>) Nuclei were contrasted with DAPI (in blue).</p