Variables' contributions to the two principal components (Cronbach's α, a measure by default for the internal consistency of categorical principal components in the SPSS software, is 0.891 and 0.721), absolute values above 0.500 were retained to define the principal components.

<p>Variables' contributions to the two principal components (Cronbach's α, a measure by default for the internal consistency of categorical principal components in the SPSS software, is 0.891 and 0.721), absolute values above 0.500 were retained to define the principal components.</p

Data describing patients' history of disease, muscle and cognitive functions (quantitative variables are mean±SD).

<p>Data describing patients' history of disease, muscle and cognitive functions (quantitative variables are mean±SD).</p

Additional file 3: of miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context

Table S1. Predictive candidate miRNA binding sites on the human NOS1 3’UTR (DOCX 12 kb

Simplified indicators of moderate pure motor DMD (cluster C) derived from series 1.

*<p>Yate's Chi square: all p = 0.00001, exact 95% confidence limits.</p>**<p>PPV and NPV, positive or negative predictive value.</p>***<p>III borderline normal, IV normal (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004347#s2" target="_blank">methods</a>).</p

Additional file 4: of miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context

Figure S1. Nuclear localization of nNOS in DMD muscular biopsy and in myoblasts. a) Control (ctrl) and DMD human muscular biopsy sections immunolabeled with anti-nNOS (red) antibody, nuclei with Dapi (blue), and imaged by confocal microscopy. Representative of 4 DMD patients. b) nNOS, GAPDH, and histone H3 (H3) immunoblots on cytoplasmic (CE) and nuclear (NE) protein extracts from control (ctrl) and DMDd45-52 myoblasts and total extract of control human muscular biopsy (ctrl biopsy). (TIFF 912 kb

The 4-cluster solution.

<p>DMD patients were assigned to 4 clusters in the final hierarchical cluster analysis (linkage: Ward's method, metric: squared Euclidean distance) using CatPCA-derived XY coordinates in a plane defined by the cognition and motor function principal components (axes) (Cronbach's α: 0.859 and 0.721, respectively). Cognition was always altered to various extent in patients with early infantile DMD (A) and classical DMD (B), but was preserved in patients with moderate (C) and severe (D) pure motor DMD who differed markedly in motor function impairment. Overall, cluster A patients were most severely affected.</p

Genotype/phenotype correlations.

<p>Proportion of patients with a mutation upstream to exon 30 steadily increased from group A to D. This ascent correlated with spared cognition (mental status: p<0.0003) but not with motor function (age at ambulation loss: NS) (Fisher's exact test). Expectedly, the 3 patients with mutation after exon 63 affecting the brain specific DP71 transcript were classified in group A.</p

Simplified indicators applied to congenital and to moderate pure motor DMD in series 2.

*<p>Yate's Chi square: all p = 0.00001, exact 95% confidence limits.</p>**<p>PPV and NPV, positive or negative predictive value.</p>***<p>I severe mental retardation, II mild mental retardation, III borderline normal, IV normal (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004347#s2" target="_blank">methods</a>).</p

Additional file 1: of miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context

Supplementary methods. (DOCX 12 kb

Simplified indicators of congenital DMD (cluster A) derived from series 1.

*<p>Yate's Chi square: all p = 0.00001, exact 95% confidence limits.</p>**<p>PPV and NPV, positive or negative predictive value.</p>***<p>I: severe mental retardation, II: mild mental retardation (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004347#s2" target="_blank">methods</a>).</p