Comparison of microarray and qPCR data for eight genes in pigs divergently selected for low or high residual feed intake (RFI).

<p>Transcriptomic differences between the two lines were validated by qPCR at 132 and 74 days of age (onset of the growing period). For values related to microarray, the highest P-value is reported when several probes are differentially expressed for a unique gene (*P < 0.05; **P < 0.01; ***P<0.001). Fold change value is expressed as the expression ratio of low RFI to high RFI samples; ratio was inversed and preceded by a minus sign for value less than 1 (i.e., a ratio of 0.5 is expressed as -2). <i>CD40</i>, tumor necrosis factor receptor superfamily member 5; <i>GPX3</i>, glutathione peroxidase 3; <i>OAZ3</i>, ornithine decarboxylase antizyme 3; <i>DGAT2</i>, diacylglycerol O-acyltransferase 2; <i>NMI</i>, N-myc interactor; <i>TRAF6</i>, TNF receptor-associated factor 6; <i>SLPI</i>, secretory leukocyte peptidase inhibitor; <i>PSEN1</i>, presenilin 1.</p

Comparison of microarray and qPCR data for three genes in pigs fed the HF or LF diet.

<p>For values related to microarray, the highest P-value is reported when several probes are differentially expressed for a unique gene (*P < 0.05; **P < 0.01; ***P < 0.001). Fold change value is expressed as the expression ratio of HF (high fiber high fat, n = 24) to LF (low fiber low fat, n = 24) diets; ratio was inversed and preceded by a minus sign for value less than 1 (i.e., a ratio of 0.5 is expressed as -2). <i>LCN2</i>, lipocalin 2; <i>CPT1A</i>, Carnitine palmitoyltransferase 1A; <i>PSAP</i>, prosaposin.</p

Additional file 4: Table S4. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

Detail list of genes associated with different biological process within a small interconnected module in perirenal adipose tissue of pigs fed a high-fat high-fiber diet. (XLSX 11 kb

Additional file 6: Table S6. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

Complete list of key upstream regulators proposed in response to diets in subcutaneous adipose tissue and associated biological processes. (XLSX 18 kb

Additional file 7: Table S7. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

Primers used for qPCR analysis. (DOCX 17 kb

Additional file 2: Table S2. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

Summary of the biological processes shared by genes being commonly regulated by diet across adipose tissue. (DOCX 23 kb

Additional file 1: Table S1. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

List of differentially expressed probes commonly regulated by diets across perirenal and subcutaneous adipose tissues. (XLSX 207 kb

Additional file 3: Table S3. of Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

List of co-expressed probes within a small module mainly attributed to perirenal adipose tissue related responses to diet. (XLSX 40 kb

Adipocyte differentiation and cellularity.

<p>Abbreviation used: LD, <i>longissimus dorsi</i> muscle; PRAT, perirenal fat; SCAT dorsal subcutaneous adipose tissue.</p><p>^aPigs (42 d of age) were fed either a methionine-deficient (MD) diet or a control (CTRL) diet providing an adequate level of methionine for 10 days. Values are least square means together with residual standard deviation (RSD).</p><p>^bmRNA levels (arbitrary units) of target genes were measured by qPCR.</p><p>^cAdipocyte mean area (μm²) was assessed on histological cross-section of adipose tissue. Total number of adipocyte was estimated per gram of adipose tissue.</p><p>Adipocyte differentiation and cellularity.</p

Antioxidant capacities in plasma.

<p>Pigs (42 d of age) were fed either a methionine-deficient (MD) or a CTRL diet providing an adequate level of methionine for 10 days (n = 6 per diet). Values are least square means together with residual standard deviation (RSD). Differences between groups were significant at ***<i>P</i>≤0.001; **<i>P</i>≤0.01 and *<i>P</i>≤0.05. Activity level of total superoxide dismutase (SOD) was expressed in units per mL (U/mL). The antioxidant activities (mmol or μmol equivalent Trolox/L) were determined by 3-ethyl-benzothiazoline-6-sulfonic acid (ABTS), the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the Ferric Reducing Ability of Plasma (FRAP) tests.</p