Volume 1, Number 12, December, 1865

https://place.asburyseminary.edu/reposofholiness/1011/thumbnail.jp

Volume 1, Number 07, July, 1865

https://place.asburyseminary.edu/reposofholiness/1006/thumbnail.jp

Volume 1, Number 11, November, 1865

https://place.asburyseminary.edu/reposofholiness/1010/thumbnail.jp

Volume 1, Number 09, September, 1865

https://place.asburyseminary.edu/reposofholiness/1008/thumbnail.jp

Volume 1, Number 08, August, 1865

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Volume 1, Number 10, October, 1865

https://place.asburyseminary.edu/reposofholiness/1009/thumbnail.jp

Phosphatase Rtr1 Regulates Global Levels of Serine 5 RNA Polymerase II C-Terminal Domain Phosphorylation and Cotranscriptional Histone Methylation

In eukaryotes, the C-terminal domain (CTD) of Rpb1 contains a heptapeptide repeat sequence of (Y1S2P3T4S5P6S7)n that undergoes reversible phosphorylation through the opposing action of kinases and phosphatases. Rtr1 is a conserved protein that colocalizes with RNA polymerase II (RNAPII) and has been shown to be important for the transition from elongation to termination during transcription by removing RNAPII CTD serine 5 phosphorylation (Ser5-P) at a selection of target genes. In this study, we show that Rtr1 is a global regulator of the CTD code with deletion of RTR1 causing genome-wide changes in Ser5-P CTD phosphorylation and cotranscriptional histone H3 lysine 36 trimethylation (H3K36me3). Using chromatin immunoprecipitation and high-resolution microarrays, we show that RTR1 deletion results in global changes in RNAPII Ser5-P levels on genes with different lengths and transcription rates consistent with its role as a CTD phosphatase. Although Ser5-P levels increase, the overall occupancy of RNAPII either decreases or stays the same in the absence of RTR1 Additionally, the loss of Rtr1 in vivo leads to increases in H3K36me3 levels genome-wide, while total histone H3 levels remain relatively constant within coding regions. Overall, these findings suggest that Rtr1 regulates H3K36me3 levels through changes in the number of binding sites for the histone methyltransferase Set2, thereby influencing both the CTD and histone codes

Pf7: an open dataset of Plasmodium falciparum genome variation in 20,000 worldwide samples

We describe the MalariaGEN Pf7 data resource, the seventh release of Plasmodium falciparum genome variation data from the MalariaGEN network.  It comprises over 20,000 samples from 82 partner studies in 33 countries, including several malaria endemic regions that were previously underrepresented.  For the first time we include dried blood spot samples that were sequenced after selective whole genome amplification, necessitating new methods to genotype copy number variations.  We identify a large number of newly emerging crt mutations in parts of Southeast Asia, and show examples of heterogeneities in patterns of drug resistance within Africa and within the Indian subcontinent.  We describe the profile of variations in the C-terminal of the csp gene and relate this to the sequence used in the RTS,S and R21 malaria vaccines.  Pf7 provides high-quality data on genotype calls for 6 million SNPs and short indels, analysis of large deletions that cause failure of rapid diagnostic tests, and systematic characterisation of six major drug resistance loci, all of which can be freely downloaded from the MalariaGEN website

Too good to be used: analyzing utilization of the test program for certain commercial items in the Air Force

MBA Professional ReportThe purpose of this project is to analyze the Air Force’s usage rate of the Federal Acquisition Regulation (FAR) Subpart 13.5 Test Program for Certain Commercial Items. FAR 13.500(b) requires contracting officers to maximize the use of the test program when practicable. In addition to the FAR mandate, the Government Accountability Office (GAO), found it is in the government’s best interest to use the program. According to a GAO report conducted in February of 2014, titled Commercial Item Test Program Beneficial, but Actions Needed to Mitigate Potential Risks, the program improved contract lead-time and reduced required administration without an increase in overall risk to the government. Therefore, underutilization of the program will identify inefficiencies in the procurement process. This research seeks to use the Federal Procurement Data System–Next Generation (FPDS–NG) data to identify the usage rate of the FAR Subpart 13.5 Test Program for Certain Commercial Items in the Air Force and provide potential recommendations to increase and improve the test program’s use. .http://archive.org/details/toogoodtobeusedn1094544561Outstanding ThesisCaptain, United States Air ForceApproved for public release; distribution is unlimited