211 research outputs found
Caracterización lesional y etiológica de neumonías en vacuno de cebo sacrificado en matadero
Trabajo presentado a la: XXVII Reunión de la Sociedad Española de Anatomía Patológica Veterinaria. (Barcelona, España, 17-19 junio de 2015).Peer Reviewe
Assessment of acute-phase protein response associated with the different pathological forms of bovine paratuberculosis
11 páginas, 1 tabla.In this study, the concentrations of two acute-phase proteins (APPs), haptoglobin (Hp)
and serum amyloid A (SAA), were quantitatively assessed in serum samples from cattle naturally
infected with paratuberculosis (PTB). APP profiles were compared across 190 animals classified
according to the different pathological forms associated with infection: uninfected (n = 59), with
focal lesions (n = 73), multifocal lesions (n = 19), and diffuse paucibacillary (n = 11) and diffuse
multibacillary lesions (n = 28). Our results showed a significant increase in both APPs in infected
animals compared to the control group, with differences depending on the type of lesion. Hp and SAA
levels were increased significantly in all infected animals, except in cows with diffuse multibacillary
lesions that showed similar values to non-infected animals. The expression pattern of both APPs
was similar and negatively correlated with the antibody levels against PTB. These results indicate
that the release of Hp and SAA is related to the presence of PTB lesions associated with a high
cell-mediated immune response and a lower bacterial load, suggesting that the pro-inflammatory
cytokines that are associated with these forms are the main stimulus for their synthesis. These
molecules could show some potential to be used as putative biomarkers of PTB infection, particularly
for the identification of subclinical animals showing pathological forms related to latency or resistance
to the development of advanced lesions.This study was funded by grants RTI2018-099496-B-I00 from the Spanish Ministry of Science and
Innovation and LE259/P18 from “Junta de Castilla y León”
Analysis of local and peripheral immune response developed in sheep experimentally infected with Toxoplasma gondii at different times of gestation
Trabajo presentado al: 4th International Meeting on Apicomplexa in Farm
Animals. (11-14 October 2017 - Madrid, Spain).Toxoplasmosis is one the main infectious causes of reproductive failure in sheep where the time
of gestation when sheep are infected affects the clinical and lesional outcome of the disease. In
order to investigate the variations of local and peripheral immune responses during gestation,
pregnant sheep were infected at early, mid and late gestation and subsequently culled at 2, 3
and 4 weeks post infection. In those sheep inoculated during the second term, serological
antibodies were detected earlier and the increase in serological γ-IFN was higher than in the
other infected animals. Regarding the local immune response at the placenta, infiltration of
inflammatory cells was mainly found in the maternal septa, although it also invaded foetal
mesenchyme adjacent to the lesions. The increase in the number of T lymphocytes was
observed only in ewes infected during the second and last terms of gestation while the increase
of B cells occurred in sheep infected at the first and second terms. The expression of iba-1
antigen by macrophages was more frequent after infection during the first term whereas
macrophages expressing lysozyme, CD163 or calprotectin were more frequent in infections at
mid-gestation. A significant increase in the transcription of γ-IFN, when compared to control
animals, occurred after infections in the first and second term, while TNF-α and IL-10
transcription increased only in the second and last term, respectively. There were no differences
when comparing transcription of cytokines between animals infected at different terms of
gestation. This study shows that the time of gestation when infection occurs has a clear influence
over the pathogenesis of ovine toxoplasmosis as a greater inflammatory response was found
after inoculating sheep at the second term of gestation. This finding may explain the later
invasion of the placenta by the parasite at early and mid gestation described in previous studies.This study was founded by AGL2016-75935-C2-2-R and LE080U16 research grants.Peer Reviewe
Influencia de la variabilidad en la virulencia de diferentes aislados de Toxoplasma gondii sobre las lesiones de encéfalos fetales ovinos
Trabajo presentado a la: XXXII Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV). 1 octubre. Congreso virtual.Peer reviewe
Infección experimental por Toxoplasma gondii en el primer, segundo y último tercio de gestación en ovejas. Respuesta lesional y distribución del parásito
6 páginas.--Trabajo presentado al: XL Congreso Nacional y el XVI Congreso Internacional de la Sociedad Española de Ovinotecnia y Caprinotecnia. (Castellón de la Plana, España, 16-18 septiembre 2015).Peer Reviewe
Foetal periventricular leucomalacia as the main lesion in abortion during the acute phase of ovine toxoplasmosis
Trabajo presentado al: ApiCOWplexa in Farm Animals/ 3rd lnternational Meeting on Apicomplexan Parasites in Farm Animals. (Edimburgo, 30th Junio al 3rd Julio, 2015).Peer Reviewe
Calicophoron daubneyi (Paramphistomidae) in slaughtered cattle in Castilla y León (Spain)
The prevalence and aetiology of natural paramphistomosis was investigated in cattle slaughtered in the Castilla y León region (Spain) over a 3 year-period. The overall prevalence of positive animals was 6.20%. The parasite burden per animal ranged from 8 to 8005 (median = 144) and the ruminal atrium had the highest parasite burden whereas the ruminal dorsal sac the lowest. The prevalence and parasite burden increased with age while these parameters were lower in cattle under intensive management. Calicophoron daubneyi was the only Paramphistomidae species identified using morphoanatomical, histological and molecular methods in the studied animals.This work was supported by grant LE023A10-2 fromJunta de Castilla y León (JCyL). A.M. Martínez-Ibeas was supported by the JCyL andthe European Social Funds (ESF) and J. Benavides by theJAE-Doc programme (CSIC-ESF).Peer Reviewe
Dicrocoeliidae family: major species causing veterinary diseases
Editado por: Toledo, R.; Fried, B.--Digenetic Trematodes.-- Colección: Advances in Experimental Medicine and Biology 766Peer Reviewe
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