qRT-PCR of infestin.

<p>Adult insects infected with <i>T. cruzi</i> and uninfected <i>T. infestans</i> were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired <i>t</i> test was performed for statistical analysis.</p

Functional classification of transcripts that originated from downregulated contigs.

<p>Functional classification of transcripts that originated from downregulated contigs.</p

A pie chart showing the species distribution of BLASTx hits of the <i>T. infestans</i> clusters for several organisms.

<p>A pie chart showing the species distribution of BLASTx hits of the <i>T. infestans</i> clusters for several organisms.</p

Functional classification of transcripts that originated from upregulated contigs.

<p>Functional classification of transcripts that originated from upregulated contigs.</p

qRT-PCR of four transcripts upregulated upon <i>T. cruzi</i> infection.

<p>Amounts of mRNA from nitrophorin-like protein (A), 14 kDa protein (B), lysozyme (C) and cathepsin D (D) were obtained by relative quantification. Adult insects infected with <i>T. cruzi</i> and uninfected <i>T. infestans</i> were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired <i>t</i> test was performed for statistical analysis, and differences were considered significant at P<0.05. Asterisks represent significant differences (** P<0.01; *** P<0.001).</p

Functional classification of transcripts from all clusters.

<p>Functional classification of transcripts from all clusters.</p

Analysis summary of <i>T. infestans</i> ESTs.

<p>Analysis summary of <i>T. infestans</i> ESTs.</p

A list of contigs with unchanged expression that are important for metabolic processes or defense in <i>T. infestans</i>.

<p>FABL – Fatty acid binding lipocalin.</p><p>JHBP – Juvenile hormone binding protein.</p

Length distribution of <i>T. infestans</i> clusters.

<p>Length distribution of <i>T. infestans</i> clusters.</p

qRT-PCR of Thioredoxin reductase upon <i>T. cruzi</i> infection.

<p>The levels of mRNA from Thioredoxin reductase were obtained by relative quantification. Adult insects infected with <i>T. cruzi</i> and uninfected <i>T. infestans</i> were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired <i>t</i> test was performed for statistical analysis, and differences were considered significant at P<0.05. Asterisks represent significant differences (*** P<0.001).</p