14 research outputs found
Serumic concentration of cardiac troponin and some enzymes in horses with strangles
This study was conducted on horses to investigate the effect of strangles on cardiac troponin fluctuations and activity of some serum enzymes. The research was done on 30 horses with strangles and 29 normal horses. Sick horses were confirmed on the basis of clinical and laboratory signs (culture of nasal and lymph nodes discharges for Streptococcus equi). Blood samples were taken from the jugular vein. The serum activity of cardiac troponin was measured by ELISA kit and gama gluthamil transferase (GGT), alanine amino tramsferase (ALT), aspartate amino transferase (AST), creatine kinase (CK) and alkaline phosphatase (ALP) were measured by biochemical kits. The mean heart rate increased significantly (
Evaluation of cardiac injury biomarkers in sheep with acute lactic acidosis
Rumen lactic acidosis is a metabolic disorder which develops in ruminants that have ingested large amounts of unaccustomed feeds rich in ruminally fermentable carbohydrates. In this study we investigated the relationship between serum lactate levels and ruminal fluid pH with changes of cardiac damage biomarkers in serum in 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy ones. After confirmation of ARLA through clinical examination and ruminal fluid pH£5.5, venous blood samples were collected and special analysis carried out on serum samples. According to the findings ruminal fluid pH in diseased and healthy sheep were 5.28 ± 0.2 and 6.93±0.3, respectively. Serum lactate level in sheep with ARLA was significantly higher than normal sheep. (p=0.000). cTnI levels was 0.684±0.03 ng/ml in sheep with ARLA, which was significantly (p=0.000) higher than healthy sheep (0.005±0.00 ng/ml). Other cardiac biomarkers were increased in diseased group, however only elevation of serum activities of AST and CK-MB were statistically significant (p=0.002 and p=0.007 respectively). Although serum LDH activity in diseased group was higher than control group; but this difference was statistically non-significant (p=0.063). There was significant negative correlation between ruminal fluid pH with cTnI concentrations (p=0.004; r=–0.850); so that highest levels of cTnI (2.28 to 3.06 ng/ml) were recorded in three sheep with ruminal fluid p
Study of the structure of buffalo's eye lens and scleroretinal rim in Tabriz by ultrasonography
In this study, ultrasonographic condition of lens and posterior wall of buffalo's eye in Tabriz area were investigated due to their important role in vision and also measurements were made in anterior-posterior axial B-mode display methods. The ultrasonographic images were similar to the images obtained from other particularly the cow. Total thickness of the lens was 1.133 ± 0.052 cm with the thickness being 1.135 ± 0.052 cm in the right eye and 1.132 ± 0.053 cm in the left eye. Thickness of the posterior wall of the right and left eyes were 1.677 ± 0.042 mm and 1.672 ± 0.041 mm respectively with the total thickness of the posterior wall measuring 1.674 ± 0.040 mm. There was no significant difference between the left and right eyes regarding these parameters
Effect of oral supplement of zinc and copper on lameness and reproductive performance in dairy cattle
The aim of this study was to evaluate the effects of oral supplements of zinc and copper on lameness and reproductive performance in dairy cattle. Performance indicators on a dairy farm are very different. In this study which was conducted on 40 Holstein cows at one of Amol's dairy farms, some of these indicators include lameness, abortion; retained placenta and open days were studied. 360 mg zinc and 125 mg copper were added to rations of daily 20 heads of cattle (case group) daily for one year. Control group was given same diet without the supplement. Regular and seasonal blood sampling were done and serum levels of zinc and copper were analyzed. The results suggested that lameness (
Hemolytic anemia due to experimental onion poisoning in Iranian Ghezel sheep
This study was conducted on 10 male Iranian Ghezel sheep. To ensure the health of livestock and deworming treatment, sheep were kept without any intervention and on a normal and high quality diet for a month. Then onion was added to the animal's diet gradually, and after a week sheep were fed only with onions. Animals were fed with onions for 10 weeks, regular and weekly blood sampling was done during this period. Clinical findings included anemia, paleness of mucus membranes, tachycardia, weakness, lethargy and death in some cases. Red blood cell counts, hemoglobin and hematocrit decreased from week 1, reaching to a significant low on week 3 (
Electrocardiographic findings in cattle with theileriosis
Theileriosis is an important protozoal disease of domestic ruminants especially cattle, sheep and goats which is transmitted by various species of Theileria infected ticks. Anemia, electrolytic disorders and vasculitis are amongst the cardiovascular complications of theileriosis. In this study, 90 cows with theileriosis were evaluated by clinical examination, parasitologic tests and electrocardiography. In Theileria infected cattle, after 30 minutes of rest and any treatment, a 30 second ECG was recorded in lead I to determine the types of arrhythmias present. Based on electrocardiographic findings frequently observed arrhythmias included 62 cases of sinus tachycardia, 15 cases of sinus arrhythmia, 6 cases of first degree atrio-ventricular block, 1 case of ventricular extra systole and 1 case of atrial fibrillation. According to the type of arrhythmias, it can be concluded that arrhythmias in cows with theileriosis are functional and nonpathologic
Comparison of Antibacterial Activities of Walnut (Juglans regia L.) and Pine (Pinus halepensis Mill.) Leaves Alcoholic Extracts against Bacteria Isolated from Burn Wound Infections
<p>The threat of infections caused by drug resistant microorganisms is a global problem, so it is essential to carry out research on alternative antimicrobial drugs. Burn wound is an ideal environment for the development of drug resistant microorganisms. Walnut (Juglans regia L.) and pine (Pinus halepensis Mill.) leaves are ancient plants with phytochemical biological compounds. The aims of this study were to evaluate the antibacterial effects of walnut and pine leaves alcoholic extracts against bacteria isolated from burn wounds infections, and compare them with selected antibiotics. Accordingly, the ethanolic extracts of walnut and pine leaves were prepared, analyzed using Agilent 7890B gas chromatography, and main phytochemicals compounds of them were identified. The antibacterial activities of alcoholic extracts against clinical isolates (n=6 isolates for each bacteria) and standard strains of Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Acinetobacter baumannii, Escherichia coli, Staphylococcus epidermidis and Staphylococcus saprophyticuswere determined by agar diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The result of this study showed that the walnut and pine leaves extract had antimicrobial activity against all above clinical isolates. In conclusion, the findings of this study showed that the walnut leaves extract had more antibacterial activities than pine leaves extract, but generally, both extracts were able to compete with the selected antibiotics of this study.</p>
Serum Levels of Zinc, Copper and Their Carrier Proteins in Cattle with theileriosis
In this study, 90 cattle with theileriosis and 90 healthy cattle were studied based on clinical and laboratory examination including parasitological and biochemical tests. Special biochemical kits were used for determination of zinc, copper, albumin, calcium, magnesium and ferrous levels in sera. Serum levels of transferin and ceruloplasmin were measured with ELISA and Sunderman & Nomoto method, respectively. The serum level of zinc was significantly decreased in cattle suffering from theileriosis (