A procedure for implanting a spinal chamber for longitudinal in vivo imaging of the mouse spinal cord.

Studies in the mammalian neocortex have enabled unprecedented resolution of cortical structure, activity, and response to neurodegenerative insults by repeated, time-lapse in vivo imaging in live rodents. These studies were made possible by straightforward surgical procedures, which enabled optical access for a prolonged period of time without repeat surgical procedures. In contrast, analogous studies of the spinal cord have been previously limited to only a few imaging sessions, each of which required an invasive surgery. As previously described, we have developed a spinal chamber that enables continuous optical access for upwards of 8 weeks, preserves mechanical stability of the spinal column, is easily stabilized externally during imaging, and requires only a single surgery. Here, the design of the spinal chamber with its associated surgical implements is reviewed and the surgical procedure is demonstrated in detail. Briefly, this video will demonstrate the preparation of the surgical area and mouse for surgery, exposure of the spinal vertebra and appropriate tissue debridement, the delivery of the implant and vertebral clamping, the completion of the chamber, the removal of the delivery system, sealing of the skin, and finally, post-operative care. The procedure for chronic in vivo imaging using nonlinear microscopy will also be demonstrated. Finally, outcomes, limitations, typical variability, and a guide for troubleshooting are discussed

A Window in the Dark Matter Exclusion Limits

We consider the cross section limits for light dark matter candidates ($m=0.4$ to 10 GeV). We calculate the interaction of dark matter in the crust above underground dark matter detectors and find that in the intermediate cross section range, the energy loss of dark matter is sufficient to fall below the energy threshold of current underground experiments. This implies the existence of a window in the dark matter exclusion limits in the micro-barn range.Comment: 10 pages, 8 figure

Detecting Gluino-Containing Hadrons

When SUSY breaking produces only dimension-2 operators, gluino and photino masses are of order 1 GeV or less. The gluon-gluino bound state has mass 1.3-2.2 GeV and lifetime > 10^{-5} - 10^{-10} s. This range of mass and lifetime is largely unconstrained because missing energy and beam dump techniques are ineffective. With only small modifications, upcoming K^0 decay experiments can study most of the interesting range. The lightest gluino-containing baryon (uds-gluino) is long-lived or stable; experiments to find it and the uud-gluino are also discussed.Comment: 13 pp, 1 figure (uuencoded). Descendant of hep-ph/9504295, hep-ph/9508291, and hep-ph/9508292, focused on experimental search techniques. To be published in Phys Rev Let

Living roots magnify the response of soil organic carbon decomposition to temperature in temperate grassland.

Increasing atmospheric carbon dioxide (CO2) concentration is both a strong driver of primary productivity and widely believed to be the principal cause of recent increases in global temperature. Soils are the largest store of the world's terrestrial C. Consequently, many investigations have attempted to mechanistically understand how microbial mineralisation of soil organic carbon (SOC) to CO2 will be affected by projected increases in temperature. Most have attempted this in the absence of plants as the flux of CO2 from root and rhizomicrobial respiration in intact plant-soil systems confounds interpretation of measurements. We compared the effect of a small increase in temperature on respiration from soils without recent plant C with the effect on intact grass swards. We found that for 48 weeks, before acclimation occurred, an experimental 3 °C increase in sward temperature gave rise to a 50% increase in below ground respiration (ca.0.4 kg C m−2; Q10=3.5), whereas mineralisation of older SOC without plants increased with a Q10 of only 1.7 when subject to increases in ambient soil temperature. Subsequent 14C dating of respired CO2 indicated that the presence of plants in swards more than doubled the effect of warming on the rate of mineralisation of SOC with an estimated mean C age of ca.8 y or older relative to incubated soils without recent plant inputs. These results not only illustrate the formidable complexity of mechanisms controlling C fluxes in soils, but also suggest that the dual biological and physical effects of CO2 on primary productivity and global temperature have the potential to synergistically increase the mineralisation of existing soil C

Diary of Colonel and Mrs. I.N. Ebey

"Whidby Island has always figured in the history of the Puget Sound country…No further attempt at settlement was made until the arrival of Isaac N. Ebey, in 1850.

Senator Cole and the Purchase of Alaska

"The person selected to broach the subject [of the Alaska purchase] was Cornelius Cole, senator-elect from California.

The Background of the Purchase of Alaska

"How nice it would be to own the entire Pacific Coast to the Arctic. With the settlement of the Oregon Question, by dividing the Oregon Country, Alaska loses this significance, and its acquisition is not seriously contemplated until the Civil War, when it is desired as a base for naval stations in the Pacific, and purchased for that purpose.