Transgenic Maize Hybrid Crop Residues: Nutritive Value and Their Effects on Performance of Grazing Beef Cows

Several maize (Zea mays) hybrids, including three transgenic hybrids containing Bacillus thuringiensis (Bt) enzymes (Bt-maize hybrids), were planted in a field study. Mature, midgestation, beef cows (Bos tarus) strip-grazed post-harvest residues as paddocks over 126 days. Body condition-scores and weights were monitored. Forage yield, weathering losses and forage composition were monitored on grazed and ungrazed areas. Forage selection and intake were estimated. Grain yields, dropped grain, initial crop residue concentrations of dry matter (DM), organic matter (OM) and in vitro digestible organic matter (IVDOM) yields were similar among hybrids. Hybrids varied in infestation of maize borers, initial amounts of residue DM, acid detergent fiber (ADF) and acid detergent lignin (ADL) and IVDOM. Mean rates of change in forage and concentration of IVDOM selected by steers was similar between hybrids. Cows grazing residues required 50% less supplemental hay to retain the same body condition as cows consuming hay in drylot

A data-driven classification of outcome behaviors in those who cause concern to British public figures

Fixated individuals pose a significant threat to public figures. Previous research compares individuals labeled ‘approachers’ to those labeled ‘communicators.’ Typically, such studies compare a number of risk factors among the two groups to identify significant differences. This has impactful implications for the threat assessment and management of the pathologically fixated. The present study builds upon this established body of work by considering if more nuance can be disaggregated from a universe of cases referred to the Fixated Threat Assessment Center (FTAC). FTAC is a joint police and mental health unit in the U.K. which applies a public health approach to managing the pathologically fixated. The present study takes a deductive approach to detect profiles of cases from the data. First, we use cluster analysis to detect unmeasured subgroups of concerning behavior in the case files of 2,118 referrals to FTAC. We identify 5 patterns of concerning behavior: incoherent offline communicators, incoherent online communicators, angry/abusive communicators, concerning approachers, and problematic approachers. Second, we examine the rate of diagnosed mental disorder, the nature of concerns evoked, and case management actions taken among each of the five profiles identified. We conclude by highlighting how our results could inform triaging large volumes of cases, the allocation of limited resources, and more generally, how the success of the FTAC model has relevance across the management of grievance-fueled violence in general. (PsycInfo Database Record (c) 2021 APA, all rights reserved

Predictors of varying levels of risks posed by fixated individuals to British public figures

Concerning approaches and communications to the Royal Family and other British public figures are relatively numerous. This paper examines over 2000 such cases logged over a three-year period in the United Kingdom. Using police and health data, the paper conducts a series of bivariate and multivariate analyses to demonstrate the predictors of what types of risk are posed by an individual case (e.g., communicate only, approach, security breach). The results showed that (a) the rates of serious mental disorders are higher among this sample than the general population base rate, (b) approachers were significantly more likely than communicators to suffer from serious mental disorders, (c) approachers were significantly more likely than communicators to have a history of substance use and abuse problems, (d) approachers were significantly more likely than communicators to have a history of violent behavior against property and persons, and (e) the motivations of approachers and communicators significantly differ. The paper concludes with a consideration of the implications for threat assessment and management

The Claims Culture: A Taxonomy of Industry Attitudes

This paper presents an analysis of a familiar aspect of construction industry culture that we have dubbed 'the claims culture'. This is a culture of contract administration that lays a strong emphasis on the planning and management of claims. The principal elements of the analysis are two sets of distinctions. The first comprises economic and occupational orders, referring to two kinds of control that are exercised over the construction process; predicated respectively on economic ownership and occupational competence. The second refers to contrasting attitudes towards relationships and problem solving within these orders: respectively 'distributive' and 'integrative'. The concepts of economic and occupational order entail further sub-categories. The various attitudes associated with these categories and sub-categories are described. They are assessed as to their consequences for change initiatives in the industry

Comparison of Dinitrogen Fixation and Nitrogen Transfer Potentials of Four Red Clover Cultivars

The capacity to fix atmospheric N2 and thus reduce the use of N fertilizer is an important reason for including legumes in forage mixtures. Selection for more efficient N2 fixation could improve red clover (Trifolium prateme L.) (RC) production and its contribution to soil fertility. An isotope dilution study was conducted in two greenhouse experiments to compare the N2 fixation and N transfer potentials of three commercial and one experimental RC cultivars. The experimental cultivar APR-8701 was selected for traits related to increased N2 fixation (rate of acetylene reduction, root size and branch number, and nodule mass). \u27Dawn\u27 orchardgrass (Dactylis glomerata L.) (OG) was used as the grass in mixture and as the reference crop for the isotope dilution study. Four harvests were taken from each of the two experiments. Herbage was analyzed first for total N and then for isotopic composition by mass spectrometry. Under the conditions of this study, the particular traits selected for in the development of the cultivar APR-8701 resulted in a high N2 fixation rate, ranging from 88.9 to 99.8 %N derived from the atmosphere (%Ndfa) in pure and mixed stands of both experiments, that paralleled the other three cultivars tested (ranging between 86.8 and 99. 9 %Ndfa in pure and mixed stands of both experiments). However, APR-8701 was average when N transfer potentials were compared. Average %N in grass derived from the atmosphere (%Ngdfa) was 32.2 and 46.3% for OG grown with APR-8701 in experiments 1 and 2, respectively, and ranged between 30. 5 and 50.1% for OG grown with the other three cultivars. We conclude that the experimental cultivar APR-8701 showed N2 fixation rates similar to that of other superior, commercially available cultivars, however, APR-8701 only showed average N transfer capability

Large-scale manipulation of promoter DNA methylation reveals context-specific transcriptional responses and stability

BACKGROUND: Cytosine DNA methylation is widely described as a transcriptional repressive mark with the capacity to silence promoters. Epigenome engineering techniques enable direct testing of the effect of induced DNA methylation on endogenous promoters; however, the downstream effects have not yet been comprehensively assessed. RESULTS: Here, we simultaneously induce methylation at thousands of promoters in human cells using an engineered zinc finger-DNMT3A fusion protein, enabling us to test the effect of forced DNA methylation upon transcription, chromatin accessibility, histone modifications, and DNA methylation persistence after the removal of the fusion protein. We find that transcriptional responses to DNA methylation are highly context-specific, including lack of repression, as well as cases of increased gene expression, which appears to be driven by the eviction of methyl-sensitive transcriptional repressors. Furthermore, we find that some regulatory networks can override DNA methylation and that promoter methylation can cause alternative promoter usage. DNA methylation deposited at promoter and distal regulatory regions is rapidly erased after removal of the zinc finger-DNMT3A fusion protein, in a process combining passive and TET-mediated demethylation. Finally, we demonstrate that induced DNA methylation can exist simultaneously on promoter nucleosomes that possess the active histone modification H3K4me3, or DNA bound by the initiated form of RNA polymerase II. CONCLUSIONS: These findings have important implications for epigenome engineering and demonstrate that the response of promoters to DNA methylation is more complex than previously appreciated. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-022-02728-5

Microwave Assisted Synthesis of Py-Im Polyamides

Microwave synthesis was utilized to rapidly build Py-Im polyamides in high yields and purity using Boc-protection chemistry on Kaiser oxime resin. A representative polyamide targeting the 5′-WGWWCW-3′ (W = A or T) subset of the consensus Androgen and Glucocorticoid Response Elements was synthesized in 56% yield after 20 linear steps and HPLC purification. It was confirmed by Mosher amide derivatization of the polyamide that a chiral α-amino acid does not racemize after several additional coupling steps

The First 1 1/2 Years of TOTEM Roman Pot Operation at LHC

Since the LHC running season 2010, the TOTEM Roman Pots (RPs) are fully operational and serve for collecting elastic and diffractive proton-proton scattering data. Like for other moveable devices approaching the high intensity LHC beams, a reliable and precise control of the RP position is critical to machine protection. After a review of the RP movement control and position interlock system, the crucial task of alignment will be discussed.Comment: 3 pages, 6 figures; 2nd International Particle Accelerator Conference (IPAC 2011), San Sebastian, Spain; contribution MOPO01

Is the Forest Service Keeping Up With the Times?

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75358/1/j.1541-0072.1995.tb01747.x.pd