1,406 research outputs found
Determining Microscopic Viscoelasticity in Flexible and Semiflexible Polymer Networks from Thermal Fluctuations
We have developed a new technique to measure viscoelasticity in soft
materials such as polymer solutions, by monitoring thermal fluctuations of
embedded probe particles using laser interferometry in a microscope.
Interferometry allows us to obtain power spectra of fluctuating beads from 0.1
Hz to 20 kHz, and with sub-nanometer spatial resolution. Using linear response
theory, we determined the frequency-dependent loss and storage shear moduli up
to frequencies on the order of a kHz. Our technique measures local values of
the viscoelastic response, without actively straining the system, and is
especially suited to soft biopolymer networks. We studied semiflexible F-actin
solutions and, as a control, flexible polyacrylamide (PAAm) gels, the latter
close to their gelation threshold. With small particles, we could probe the
transition from macroscopic viscoelasticity to more complex microscopic
dynamics. In the macroscopic limit we find shear moduli at 0.1 Hz of G'=0.11
+/- 0.03 Pa and 0.17 +/- 0.07 Pa for 1 and 2 mg/ml actin solutions, close to
the onset of the elastic plateau, and scaling behavior consistent with G(omega)
as omega^(3/4) at higher frequencies. For polyacrylamide we measured plateau
moduli of 2.0, 24, 100 and 280 Pa for crosslinked gels of 2, 2.5, 3 and 5%
concentration (weight/volume) respectively, in agreement to within a factor of
two with values obtained from conventional rheology. We also found evidence for
scaling of G(omega) as \omega^(1/2), consistent with the predictions of the
Rouse model for flexible polymers.Comment: 16 pages, with 15 PostScript figures (to be published in
Macromolecules
Effective medium approach for stiff polymer networks with flexible cross-links
Recent experiments have demonstrated that the nonlinear elasticity of in
vitro networks of the biopolymer actin is dramatically altered in the presence
of a flexible cross-linker such as the abundant cytoskeletal protein filamin.
The basic principles of such networks remain poorly understood. Here we
describe an effective medium theory of flexibly cross-linked stiff polymer
networks. We argue that the response of the cross-links can be fully attributed
to entropic stiffening, while softening due to domain unfolding can be ignored.
The network is modeled as a collection of randomly oriented rods connected by
flexible cross-links to an elastic continuum. This effective medium is treated
in a linear elastic limit as well as in a more general framework, in which the
medium self-consistently represents the nonlinear network behavior. This model
predicts that the nonlinear elastic response sets in at strains proportional to
cross-linker length and inversely proportional to filament length. Furthermore,
we find that the differential modulus scales linearly with the stress in the
stiffening regime. These results are in excellent agreement with bulk rheology
data.Comment: 12 pages, 8 figure
Instability and front propagation in laser-tweezed lipid bilayer tubules
We study the mechanism of the `pearling' instability seen recently in
experiments on lipid tubules under a local applied laser intensity. We argue
that the correct boundary conditions are fixed chemical potentials, or surface
tensions \Sigma, at the laser spot and the reservoir in contact with the
tubule. We support this with a microscopic picture which includes the intensity
profile of the laser beam, and show how this leads to a steady-state flow of
lipid along the surface and gradients in the local lipid concentration and
surface tension (or chemical potential). This leads to a natural explanation
for front propagation and makes several predictions based on the tubule length.
While most of the qualitative conclusions of previous studies remain the same,
the `ramped' control parameter (surface tension) implies several new
qualitative results. We also explore some of the consequences of front
propagation into a noisy (due to pre-existing thermal fluctuations) unstable
medium.Comment: 12 page latex + figures using epsf.sty to be published in Journal de
Physique II, January 199
Origin of slow stress relaxation in the cytoskeleton
Dynamically crosslinked semiflexible biopolymers such as the actin
cytoskeleton govern the mechanical behavior of living cells. Semiflexible
biopolymers nonlinearly stiffen in response to mechanical loads, whereas the
crosslinker dynamics allow for stress relaxation over time. Here we show,
through rheology and theoretical modeling, that the combined nonlinearity in
time and stress leads to an unexpectedly slow stress relaxation, similar to the
dynamics of disordered systems close to the glass transition. Our work suggests
that transient crosslinking combined with internal stress can explain prior
reports of soft glassy rheology of cells, in which the shear modulus increases
weakly with frequency.Comment: 6 pages, 4 figure
Deformation of crosslinked semiflexible polymer networks
Networks of filamentous proteins play a crucial role in cell mechanics. These
cytoskeletal networks, together with various crosslinking and other associated
proteins largely determine the (visco)elastic response of cells. In this letter
we study a model system of crosslinked, stiff filaments in order to explore the
connection between the microstructure under strain and the macroscopic response
of cytoskeletal networks. We find two distinct regimes as a function primarily
of crosslink density and filament rigidity: one characterized by affine
deformation and one by non-affine deformation. We characterize the crossover
between these two.Comment: Typos fixed and some technical details clarified. To appear in Phys.
Rev. Let
Criticality and isostaticity in fiber networks
The rigidity of elastic networks depends sensitively on their internal
connectivity and the nature of the interactions between constituents. Particles
interacting via central forces undergo a zero-temperature rigidity-percolation
transition near the isostatic threshold, where the constraints and internal
degrees of freedom are equal in number. Fibrous networks, such as those that
form the cellular cytoskeleton, become rigid at a lower threshold due to
additional bending constraints. However, the degree to which bending governs
network mechanics remains a subject of considerable debate. We study disordered
fibrous networks with variable coordination number, both above and below the
central-force isostatic point. This point controls a broad crossover from
stretching- to bending-dominated elasticity. Strikingly, this crossover
exhibits an anomalous power-law dependence of the shear modulus on both
stretching and bending rigidities. At the central-force isostatic point---well
above the rigidity threshold---we find divergent strain fluctuations together
with a divergent correlation length , implying a breakdown of continuum
elasticity in this simple mechanical system on length scales less than .Comment: 6 pages, 5 figure
Non-equilibrium mechanics and dynamics of motor activated gels
The mechanics of cells is strongly affected by molecular motors that generate
forces in the cellular cytoskeleton. We develop a model for cytoskeletal
networks driven out of equilibrium by molecular motors exerting transient
contractile stresses. Using this model we show how motor activity can
dramatically increase the network's bulk elastic moduli. We also show how motor
binding kinetics naturally leads to enhanced low-frequency stress fluctuations
that result in non-equilibrium diffusive motion within an elastic network, as
seen in recent \emph{in vitro} and \emph{in vivo} experiments.Comment: 21 pages, 8 figure
Elastic response of filamentous networks with compliant crosslinks
Experiments have shown that elasticity of disordered filamentous networks
with compliant crosslinks is very different from networks with rigid
crosslinks. Here, we model and analyze filamentous networks as a collection of
randomly oriented rigid filaments connected to each other by flexible
crosslinks that are modeled as worm-like chains. For relatively large
extensions we allow for enthalpic stretching of crosslinks' backbones. We show
that for sufficiently high crosslink density, the network linear elastic
response is affine on the scale of the filaments' length. The nonlinear regime
can become highly nonaffine and is characterized by a divergence of the elastic
modulus at finite strain. In contrast to the prior predictions, we do not find
an asymptotic regime in which the differential elastic modulus scales linearly
with the stress, although an approximate linear dependence can be seen in a
transition from entropic to enthalpic regimes. We discuss our results in light
of the recent experiments.Comment: 10 pages, 11 figure
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