MOESM2 of Impact of mesenchymal stem cells’ secretome on glioblastoma pathophysiology

Additional file 2: Table S2. SWATH-MS method

MOESM1 of Impact of mesenchymal stem cells’ secretome on glioblastoma pathophysiology

Additional file 1: Table S1. Sequence of primers used for RT-qPCR analyses

MOESM4 of Impact of mesenchymal stem cells’ secretome on glioblastoma pathophysiology

Additional file 4: Figure S1. mRNA expression of genes coding for proteins detected in proteomic analyses by quantitative RT-qPCR. (A) Gel electrophoresis of CCL2, TPT1, POSTN, TGFβI, SEMA7A, PDGFC, IL6 and TBP expression in HUCPVCs. The RT-qPCR products were run on a 2% agarose gel. (B) Relative mRNA expression quantification in HUCPVCs. Data is normalized for TBP expression, and results are expressed as the mean ± SD of 3 biological replicates. M, Molecular weight marker 100 bp, ThermoScientific; #1, #2, and #3, independent biological replicates of HUCPVCs; (-), negative control

The metabolic microenvironment of melanomas: Prognostic value of MCT1 and MCT4

<p><i>BRAF</i> mutations are known drivers of melanoma development and, recently, were also described as players in the Warburg effect, while this reprogramming of energy metabolism has been identified as a possible strategy for treating melanoma patients. Therefore, the aim of this work was to evaluate the expression and prognostic value of a panel of glycolytic metabolism-related proteins in a series of melanomas. The immunohistochemical expression of MCT1, MCT4, GLUT1, and CAIX was evaluated in 356 patients presenting melanoma and 20 patients presenting benign nevi. Samples included 20 benign nevi, 282 primary melanomas, 117 lymph node and 54 distant metastases samples. <i>BRAF</i> mutation was observed in 29/92 (31.5%) melanoma patients and 17/20 (85%) benign nevi samples. <i>NRAS</i> mutation was observed in 4/36 (11.1%) melanoma patients and 1/19 (5.3%) benign nevi samples. MCT4 and GLUT1 expression was significantly increased in metastatic samples, and MCT1, MCT4 and GLUT1 were significantly associated with poor prognostic variables. Importantly, MCT1 and MCT4 were associated with shorter overall survival. In conclusion, the present study brings new insights on metabolic aspects of melanoma, paving the way for the development of new-targeted therapies.</p