Assessment of Literacy Pedagogy using Gratitude

This paper considers aspects of a course redesign that focuses on motivating Pre-service Teachers to engage in negotiating relevant literacy teaching pedagogies in their discipline. The purpose of this article is to describe how we approached the teaching of literacy with Pre-service Teachers, in ways that valued the Pre-service Teachers’ relationships with secondary students using notes of gratitude. These notes provided the Pre-service Teachers with an opportunity to communicate in plain language to the students what they learnt from them about literacy pedagogy. The shift from the focus on the subject matter of literacy to the enactment of literacy teaching and learning through valued pre-service teacher and student relationships shifted the tenor of the course. Our conclusion emphasises how this innovation in assessment enabled us to emphasise the importance of relationality in teaching and to uphold ideals of social inclusion of school students and the professional growth of Pre-service Teachers

Introducing the Fair and Logical Trade Project

We introduce our framework for logic-based composi- tional e-commerce interaction. We aim to provide open- source software which adds a light-weight formal messag- ing layer to business communications, to increase the ac- cessibility of e-commerce infrastructure to smaller business players. In the process we hope to develop a comprehensive theory of business communication. We present the logical structures and techniques we apply, and provide initial pro- totype testing results

Profiling the Human Phosphoproteome to Estimate the True Extent of Protein Phosphorylation

Mass spectrometry-based phosphoproteomics allows large-scale generation of phosphorylation site data. However, analytical pipelines need to be carefully designed and optimised to minimise incorrect identification of phosphopeptide sequences or wrong localisation of phosphorylation sites within those peptides. Public databases such as PhosphoSitePlus (PSP) and PeptideAtlas (PA) compile results from published papers or openly available MS data, but to our knowledge, there is no database-level control for false discovery of sites, subsequently leading to the likely overestimation of true phosphosites. It is therefore difficult for researchers to assess which phosphosites are “real” and which are likely to be artefacts of data processing. By profiling the human phosphoproteome, we aimed to estimate the false discovery rate (FDR) of phosphosites based on available evidence in PSP and/or PA and predict a more realistic count of true phosphosites. We ranked sites into phosphorylation likelihood sets based on layers of accumulated evidence and then analysed them in terms of amino acid conservation across 100 species, sequence properties and functional annotations of associated proteins. We demonstrated significant differences between the sets and developed a method for independent phosphosite FDR estimation. Remarkably, we estimated a false discovery rate of 86.1%, 95.4% and 82.2% within sets of described phosphoserine (pSer), phosphothreonine (pThr) and phosphotyrosine (pTyr) sites respectively for which only a single piece of identification evidence is available (the vast majority of sites in PSP). Overall, we estimate that ∼56,000 Ser, 10,000 Thr and 12,000 Tyr phosphosites in the human proteome have truly been identified to date, based on evidence in PSP and/or PA, which is lower than most published estimates. Furthermore, our analysis estimated ∼91,000 Ser, 49,000 Thr and 26,000 Tyr sites that are likely to represent false-positive phosphosite identifications. We conclude that researchers should be aware of the significant potential for false positive sites to be present in public databases and should evaluate the evidence behind the phosphosites used in their research

Industrial Additive Manufacturing: a manufacturing systems perspective

As Additive Manufacturing becomes increasingly prevalent in commercial manufacturing environments, the need to effectively consider optimal strategies for management is increased. At present most research has focused on individual machines, yet there is a wealth of evidence to suggest competitive manufacturing is best managed from a systems perspective. Through 14 case studies developed with four long-established Additive Manufacturing companies this paper explores the conduct of Industrial AM in contemporary manufacturing environments. A multitude of activities, mechanisms, and controls are identified through this detailed investigation of Additive Manufacturing operations. Based on these empirical results a general four component Industrial Additive Manufacturing System is developed, together with the identification of potential strategic opportunities to enhance future manufacturing

Using simulation to explore the influence of online reviews on supply chain dynamics

This paper extends existing research on the dynamic behaviour of supply chains by including the influence of online reviews. We model an online supply chain which contains customers and one e-commerce retailer. By using simulation, we compare the dynamic performance in a supply chain for two scenarios, namely adopting online review systems and without adopting the systems. The supply chain dynamic performance is measured by bullwhip effect and inventory variance amplification. The results demonstrate that online review systems increase both the bullwhip effect and inventory variance amplification, and this impact can be moderated by product quality, unit mismatch cost, lead time, and customer volatility. We further explore how our model could be extended to include market competition, dual sourcing, online review manipulation, and product returns. As the increase in the bullwhip effect and inventory variance amplification can be associated with supply chain inefficiency, managers who are aware of such consequence induced by online review adoption can make better decisions in supply chain management

Social media in operations and supply chain management: state-of-the-art and research directions

Recently, industrial and academic communities in the operations and supply chain management (OSCM) field are paying increasing attention to social media. However, the value of social media in OSCM is quite unclear, and more investigations are still needed. To pave the way for a directed future research, this paper systematically reviewed and synthesised 152 peer-review journal papers to identify research focus and gaps in this area, supported by an appropriate conceptual framework. The result reveals that the research interests in this area have increased dramatically within the last decade across various industries and regions. Different companies’ OSCM activities, such as sourcing and delivery, can benefit from employment of social media. This paper also indicates that future research can explore the value of social media in sourcing, delivery, product return and reverse logistics activities, forecasting and inventory management, and product development and production

3D printing the future: scenarios for supply chains reviewed

Purpose: The aim of this paper is to evaluate existing scenarios for 3D Printing in order to identify the “white space” where future opportunities have not been proposed or developed to date. Based around aspects of order penetration points, geographical scope and type of manufacturing, these gaps are identified. Design/methodology/approach: A structured literature review has been carried out on both academic and trade publications. As of the end of May 2016, this identified 128 relevant articles containing 201 future scenarios. Coding these against aspects of existing manufacturing and supply chain theory has led to the development of a framework for identify “white space” in existing thinking. Findings: The coding shows that existing future scenarios are particularly concentrated on job shop applications and pull based supply chain processes, although there are fewer constraints on geographical scope. Five distinct areas of “white space” are proposed, reflecting various opportunities for future 3DP supply chain development. Research limitations: Being a structured literature review, there are potentially articles not identified through the search criteria used. The nature of the findings is also dependent upon the coding criteria selected. However, these are theoretically derived and reflect important aspect of strategic supply chain management. Practical implications: Practitioners may wish to explore the development of business models within the “white space” areas. Originality/value: Currently, existing future 3DP scenarios are scattered over a wide, multi-disciplinary literature base. By providing a consolidated view of these scenarios, it is possible to identify gaps in current thinking. These gaps are multidisciplinary in nature and represent opportunities for both academics and practitioners to exploit

The influence of online review adoption on the profitability of capacitated supply chains

The paper explores the influence of online review adoption on supply chain profitability under the presence of a capacity constraint. Nowadays, customers increasingly rely on online reviews for decision making, and online retailers regard reviews as a norm. Although online reviews have been extensively examined in marketing disciplines, little research has been conducted to investigate their influence from a supply chain perspective. In addition, previous research has largely focused on how online review information can influence customer purchase behaviours, but ignores the more basic decision: whether and when companies should adopt reviews. This paper examines the online review adoption decision from a capacitated supply chain perspective through mathematical modelling and simulation. The simulation considers the influence of variables including online review adoption decision, capacity constraint level, lost sales penalty level, and product quality estimation on supply chain profitability. Generally, we find that online reviews can bring more profit to the supply chain than without online reviews, although such influence is moderated by the other three variables. The findings reveal the complexity of the contextual variable impacts on online review adoption, and demonstrate that decisions concerning the adoption of online reviews should take all supply-chain-related variables into consideration rather than only aiming for increasing customer orders

The impact of additive manufacturing on the product-process matrix

The relationship between volume, variety, and process choice is a fundamental tenet of manufacturing research and practice, and through the product-process matrix managers balance trade-offs between the traditionally dichotomous objectives of flexibility and cost in process selection. In this paper we examine the adherence of Additive Manufacturing systems to traditional trade-offs, and identify circumstances where they deviate from these established norms. Using engineering philosophy we develop an extension of the product-process matrix to accommodate both variety and customization measures, which is used to evaluate case study research conducted with five major Additive Manufacturing companies. Fifteen case studies inform the research, drawn from a broad range of industry sectors. A qualitative approach was taken, using semi-structured interviews and process observation. The study demonstrates that Additive Manufacturing systems can support both alignment and disjunction to established theory. For many cases a general conformance to the traditional product-process matrix ‘diagonal’ is evidenced. However, several cases show significant deviation, demonstrating the achievement of both variety and volume for both batch and line production. Through a detailed exploration of the focal cases, we highlight the characteristics of both products and Additive Manufacturing systems that can help overcome traditional trade-off constraints

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics

ABSTRACT Polo-like kinase 4 (PLK4) is the master regulator of centriole duplication in metazoan organisms. Catalytic activity and protein turnover of PLK4 are tightly coupled in human cells, since changes in PLK4 concentration and catalysis have profound effects on centriole duplication and supernumerary centrosomes, which are associated with aneuploidy and cancer. Recently, PLK4 has been targeted with a variety of small molecule kinase inhibitors exemplified by centrinone, which rapidly induces inhibitory effects on PLK4 and leads to on-target centrosome depletion. Despite this, relatively few PLK4 substrates have been identified unequivocally in human cells, and PLK4 signaling outside centriolar networks remains poorly characterised. We report an unbiased mass spectrometry (MS)-based quantitative analysis of cellular protein phosphorylation in stable PLK4-expressing U2OS human cells exposed to centrinone. PLK4 phosphorylation was itself sensitive to brief exposure to the compound, resulting in PLK4 stabilization. Analysing asynchronous cell populations, we report hundreds of centrinone-regulated cellular phosphoproteins, including centrosomal and cell cycle proteins and a variety of likely ‘non-canonical’ substrates. Surprisingly, sequence interrogation of ~300 significantly down-regulated phosphoproteins reveals an extensive network of centrinone-sensitive [Ser/Thr]Pro phosphorylation sequence motifs, which based on our analysis might be either direct or indirect targets of PLK4. In addition, we confirm that NMYC and PTPN12 are PLK4 substrates, both in vitro and in human cells. Our findings suggest that PLK4 catalytic output directly controls the phosphorylation of a diverse set of cellular proteins, including Pro-directed targets that are likely to be important in PLK4-mediated cell signaling