7 research outputs found

    Impact of diurnal variation on <i>Clock</i> expression analysis in hippocampus of epileptic rats.

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    <p>Relative amounts of <i>Clock</i> transcripts in epileptic rats ZT08 (A) and ZT12 (B) after normalization to <i>Tubb2a</i>/<i>Rplp1</i>. Significant differences were evaluated using Unpaired Student’s t-test comparing results between epileptic and each ZT of naive group. *p<0.05, **p<0.01 and ***p<0.001. Data are presented as mean+SEM (n = 4 rats in each epileptic group and 5 rats/time point in naive).</p

    Selection of the most suitable reference genes for circadian analysis in the hippocampus of rats.

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    <p>Expression stability measurements for the 8 reference genes calculated by geNorm (A) and NormFinder (B). The x-axis from left to right indicates the ranking of the genes according to their expression stability; lower values indicate higher expression stability. C) Determination of the optimal number of reference genes for normalization by geNorm. The Software calculates the normalization factor from at least two genes at which the variable V defines the pair-wise variation between two sequential normalization factors.</p

    Impact of diurnal variation on <i>Per3</i> expression analysis in hippocampus of epileptic rats.

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    <p>Relative amounts of <i>Per3</i>, transcripts in epileptic rats ZT08 (A) and ZT12 (B) after normalization to <i>Tubb2a</i>/<i>Rplp1</i>. Significant differences were evaluated using Unpaired Student’s t-test comparing results between epileptic and each ZT of naive group. *p<0.05, **p<0.01 and ***p<0.001. Data are presented as mean+SEM (n = 5 (ZT8) and 4 (ZT12) rats in epileptic group and n = 5 rats/time point in naive).</p

    Temporal expression of the core clock transcripts in the hippocampus of rats.

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    <p>A) Relative amounts of transcripts at different ZT after normalization to <i>Tubb2a</i>/<i>Rplp1</i>. Data are presented as mean (n = 5 rats/ZT). Statistical test for circandian analysis by Acrophase (left) and CirWawe (right). B) Overlap of cosine fitting curves illustrating the phase relation of clock transcripts. For clarity reasons, data are doubleblotted against <i>Zeitgeber time</i> (ZT).</p

    Impact of diurnal variation on <i>Bmal</i> expression analysis in hippocampus of epileptic rats.

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    <p>Relative amounts of <i>Bmal</i>, transcripts in epileptic rats ZT08 (A) and ZT12 (B) after normalization to <i>Tubb2a</i>/<i>Rplp1</i>. Significant differences were evaluated using Unpaired Student’s t-test comparing results between epileptic and each ZT of naive group. *p<0.05, **p<0.01 and ***p<0.001. Data are presented as mean+SEM (n = 5 (ZT8) and 4 (ZT12) rats in epileptic group and n = 5 rats/time point in naive).</p

    Primer sequences and amplification summary.

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    <p>*Clock, Circadian locomotor output cycle kaput; Cry1-2, Cryptochrome1-2; Per1-3, Period 1–3; Bmal1, Brain and muscle Arnt-like protein-1.</p><p>Primer sequences and amplification summary.</p

    Impact of diurnal variation on <i>Cry1</i> expression analysis in hippocampus of epileptic rats.

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    <p>Relative amounts of <i>Cry1</i>, transcripts in epileptic rats ZT08 (A) and ZT12 (B) after normalization to <i>Tubb2a</i>/<i>Rplp1</i>. Significant differences were evaluated using Unpaired Student’s t-test comparing results between epileptic and each ZT of naive group. *p<0.05, **p<0.01 and ***p<0.001. Data are presented as mean+SEM (n = 5 (ZT8) and 4 (ZT12) rats in epileptic group and n = 5 rats/time point in naive).</p
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