GPR120 expression by HPC and Hepatocytes in pediatric NAFLD patients.

<p>A) Immunohistochemistry for EpCAM and GPR120 in serial sections of pediatric NAFLD biopsies. CK-7-positive HPCs highly express GPR120 (arrows). B) Double immunofluorescence for EpCAM (green) and GPR120 (red) in NAFLD biopsies. Nuclei are shown in blue. Numerous EpCAM-positive progenitor cells co-express GPR120 (yellow cells: yellow arrows) confirming the findings obtained through immunohistochemistry in serial sections. C) Immunohistochemistry for GPR120 in sections of pediatric NAFLD biopsies before and after DHA treatment. A significant increase of GPR120-positive hepatocytes was clearly seen after DHA treatment. Original magnification: 40×.</p

GPR120 expressions by Macrophages in pediatric NAFLD patients.

<p>A) Immunohistochemistry for GPR120 in sections of pediatric NAFLD biopsies. B) Double immunofluorescence for CD68 (red) and GPR120 (green) in NAFLD biopsies. Nuclei are shown in blue. CD68-positive macrophages express GPR120 (yellow arrows). Original magnification: 40×. C) The histogram shows the number of GPR120-positive macrophages per high power field (HPF) at the baseline and after DHA treatment. A significant reduction of GPR120-positive macrophages was seen after DHA treatment. * = p<0.05.</p

Immunohistochemistry for Cytokeratin(CK) 7 and EpCAM in liver biopsies of pediatric NAFLD patients.

<p>A) at the beasline, pediatric NAFLD biopsies were characterized by a prominent expansion of hepatic progenitor cell (HPC) pool and the presence of reactive ductules at the periphery of portal spaces. After DHA treatment, a minimal involvement of the HPC compartment was present. Original magnification 20×. B) EpCAM-positive HPCs (arrows) were significant reduced by DHA treatment in comparison with the biopsies at the baseline. Original magnification 40×. C) Histograms show the reduction of HPC expansion (ductular reaction extension) and activation (number of EpCAM+ cells) after DHA treatment. Data are shown as Means ± Standard Deviation. * = p<0.05.</p

Nuclear expression of phosphorylated (p) NF-κB by Macrophages in pediatric NAFLD patients.

<p>A) Immunohistochemistry for serine-311-phosphorylated NF-κB (pNF-κB) in pediatric NAFLD biopsies before and after DHA treatment. In parallel with GPR120 expression, the DHA treatment determined a reduction of pNF-κB expression and its nuclear translocation in macrophages (A, yellow arrows). Original magnification: 40×. B) Histogram shows the significant reduction of pNF-κB nuclear translocation in macrophages after DHA treatment. Data are shown as Means ± Standard Deviation. * = p<0.05. C–D) The number of GPR120-positive macrophages (C) and the pNF-κB nuclear expression in macrophages. (D) were correlated with serum level of inflammatory cytokines.</p

Anthropometric and laboratory parameters of the study-population.

<p>Data are reported as Means ± SD * = p<0.05.</p

Nuclear expression of phosphorylated (p) NF-κB by hepatocytes in pediatric NAFLD patients.

<p>A) Immunohistochemistry for pNF-κB in pediatric NAFLD biopsies demonstrates the reduction of pNF-κB in hepatocyte nuclei (red arrows) after DHA treatment. B) DHA treatment determined a significant reduction of pNF-κB expression and its nuclear translocation in hepatocytes. Data are shown as Means ± Standard Deviation. * = p<0.05.</p

Correlations of total a-syn with PD patients’ clinical features.

<p>Scatter-plots showing the correlation between α-syn_total and patients’ disease duration (DD) (panel A), Movement Disorder Society-Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) (part 1–4) (panel B), Montreal Cognitive Assessment (MOCA) (panel C) and Frontal Assessment Battery (FAB) scores (panel D) in patients with PD.</p

JHC772798_Supplemental_Material – Supplemental material for Immunolocalization of Advanced Glycation End Products, Mitogen Activated Protein Kinases, and Transforming Growth Factor-β/Smads in Pelvic Organ Prolapse

<p>Supplemental material, JHC772798_Supplemental_Material for Immunolocalization of Advanced Glycation End Products, Mitogen Activated Protein Kinases, and Transforming Growth Factor-β/Smads in Pelvic Organ Prolapse by Antonella Vetuschi, Simona Pompili, Anna Gallone, Angela D’Alfonso, Maria Gabriella Carbone, Gaspare Carta, Claudio Festuccia, Eugenio Gaudio, Alessandro Colapietro and Roberta Sferra in Journal of Histochemistry & Cytochemistry</p

Salivary a-synuclein in PD patients and healthy subjects.

<p>Salivary a-syn in patients with Parkinson’s disease (PD) and healthy subjects (HS). Each histogram corresponds to the mean concentration of α-syn_total (panel A), α-syn_olig (panel B) and the α-syn_total/α-syn_olig ratio (panel C) in the saliva of patients with PD (black histogram) and HS (white histogram). Vertical bars denote standard deviation. Asterisk denote significant differences. Scatter-plot (panel D) shows the negative correlation between α-syn_total and α-syn_olig in patients with PD.</p

Correlations between total macrophage number and histo-pathological parameters in all pediatric NAFLD biopsies at baseline (N = 32).

<p>Correlations between total macrophage number and histo-pathological parameters in all pediatric NAFLD biopsies at baseline (N = 32).</p