Cell selection of tobacco and clover plants for resistance to high level of copper ions

Проведен подбор питательных сред для культивирования каллусов клевера ползучего. Подобраны селективные концентрации ионов меди для растений клевера и табака, с целью последующей клеточной селекции на устойчивость к высоким дозам тяжелых металлов. Показано, что в культуре in vitro каллусы растений обладают большей устойчивостью к действию тяжелых металлов, чем проростки растений in vivo. Получены клеточные линии, устойчивые к содержанию меди в среде более 200 мкМ. Данные культуры потеряли морфогенные свойства. Ведется анализ механизмов устойчивости и причин потери морфогенности данных культур.The culture media for white clover callus were selected. Copper ions selective concentration for tobacco and white clover plants was chosen for further cell selection for resistance to high level of heavy metals. It was shown in vitro that plant calli had greater resistance to heavy metals than the plant seedlings in vivo. Cell lines resistant to copper in concentration more than 200 mkM were obtained but the cultures lost its morphogenic properties. The resistance mechanisms and the reasons of morphogeneity loss are being conducted.Программа развития УрФУ на 2013 год (п.1.2.2.3

THE MANAGEMENT OF CORPORATE CULTURE IN RUSSIA BASED ON THE DYNAMIC CONCEPT

The arguments substantiating the need for management of corporate culture in Russia are presented. The advantages of the spiral model of development of the corporate culture are identified. The basic stages of development of corporate culture are described. The general state of the corporate culture of russian enterprises is assessed. The general recommendations for managing of corporate culture are given

THE MANAGEMENT OF CORPORATE CULTURE IN RUSSIA BASED ON THE DYNAMIC CONCEPT

The arguments substantiating the need for management of corporate culture in Russia are presented. The advantages of the spiral model of development of the corporate culture are identified. The basic stages of development of corporate culture are described. The general state of the corporate culture of russian enterprises is assessed. The general recommendations for managing of corporate culture are given

THE ROLE OF DIAGNOSIS OF CORPORATE CULTURE IN MERGER SCENARIOS

The arguments substantiating the need for diagnosis of corporate culture in the planning of the merger are presented. The basic levels of the corporate culture development are identified. The main factors influencing the development of the corporate culture are identified. Various scenarios of corporate merger are considered. The effect of differences in corporate cultures on the process of the merger are described

THE ROLE OF DIAGNOSIS OF CORPORATE CULTURE IN MERGER SCENARIOS

The arguments substantiating the need for diagnosis of corporate culture in the planning of the merger are presented. The basic levels of the corporate culture development are identified. The main factors influencing the development of the corporate culture are identified. Various scenarios of corporate merger are considered. The effect of differences in corporate cultures on the process of the merger are described

Prediction of some peroxidase functions in Arabidopsis thaliana L. By bioinformatic search

Peroxidases of class III are common in various organisms. They are involved in lignin biosynthesis and plant protection against stressors. Peroxidases are presented in many isoforms, whose role is not always clear. The aim of this study is to analyze the amino acid sequences of reference peroxidases with known functions and peroxidases from Arabidopsis thaliana L. whose functions are unknown and to consider their putative roles in lignin biosynthesis. The structural and functional organization of peroxidases was analyzed by bioinformatical methods applied to open Internet sources. Seven reference peroxidases were chosen from four plant species: Zinnia sp., Armoracia rusticana P.G. Gaertn., Lycopersicon esculentum L. N Populus alba L. Twenty-four amino acid sequences of homologous peroxidases from A. thaliana were selected for the analyses with the BLAST service. Their molecular weights and isoelectric points were calculated. Multiple alignments of amino acid sequences and phylogenetic analysis were done. Sites of binding to monolignol substrates were identified in seven peroxidases from A. thaliana, and the enzymes were assigned to the groups of S- or G-peroxidases. Amino acid replacements in the primary structures of peroxidases were analyzed. Peroxidases from A. thaliana were clustered with reference peroxidases. They formed six clusters on the phylogenetic tree, three of which contained only A. thaliana peroxidases. Peroxidases within each cluster had similar molecular weights and isoelectric points, common localization of expression, and similar functions. Thus, the use of bioinformatics, databases, and published data bring us to assumptions as to the functions of several A. thaliana class III peroxidases. AtPrx39 peroxidase was shown to be affine to sinapyl alcohol; AtPrx54, to p-coumaryl and coniferyl alcohols. They are likely to participate in lignin biosynthesis. © Tugbaeva A.S., Ermoshin A.A., Kiseleva I.S., 2019