MOESM1 of Functional genomic analyses of Enterobacter, Anopheles and Plasmodium reciprocal interactions that impact vector competence

Additional file 1: Figure S1. Impact of blood feeding Esp_Z on mosquito fitness. (A) Longevity studies were performed following blood-meal introduction of either Esp_Z, bacterial cocktail, or PBS into aseptic mosquito cohorts. A sterile blood meal was provided on day 4 (black arrow), and unfed mosquitoes were censored from the analysis. Survival was monitored daily and continued until 100 % mortality was reached. The curves represent the average percent mortality across three replicates, and the error bars indicate the standard error. Significance was determined using the log-rank test (Mantel-Cox) using a Kaplan–Meier survival analysis. (B) Fecundity analysis between Esp_Z-, bacterial cocktail- and PBS-fed blood-fed mosquito cohorts. Separate cohorts were provided a second blood meal 72 h after blood-meal introduction of Esp_Z, and circles represent the number of eggs laid per female. Horizontal bars represent the median number of eggs, error bars indicate the standard error, and three pooled biological replicates are shown. Significance was determined using the Mann–Whitney test. (C) Fertility analysis in Esp_Z-, bacterial cocktail-, and PBS-fed blood-fed mosquitoes. At 72 h after introduction, mosquitoes were offered a second, naive blood meal, and those not engorged were removed. Eggs were collected 48 h post-blood meal and allowed to hatch in rearing trays. The hatch rate indicates the percentage of eggs giving rise to 1st instar larvae; the error bars indicate the standard error of the mean, and significance was determined using an unpaired t-tes

Additional file 1: of Primordial origin and diversification of plasmids in Lyme disease agent bacteria

Figure S1. Borreliella plasmid PFam32 protein neighbor-joining tree. Figure S2. Two examples of Borreliella linear plasmids with low protein coding potential. Figure S3. Comparative maps of linear plasmids in Borreliella isolates. Maps of the following linear plasmids are shown in the following panels: A, lp5; B, lp17; C, lp25; D, lp28–2, lp28–6, lp28–7 and lp28–9; E, lp28–3; F, VS116 lp28–3; G, lp28–4; H, lp28–8; I, lp32; J, lp36; K, lp38; L, lp56. Figure S4. The PFam54 gene cluster of the Borreliella lp54 plasmids. Figure S5. Ends of the Borreliella linear chromosome sequences. Figure S6. Comparative maps of cp9 plasmids in Borreliella isolates. Figure S7. Orphan cp32-like contigs in the B. spielmanii A14S genome. Figure S8. Rearrangements in cp32-like plasmids in NBu-Borreliella genomes. Figure S9. B. bissettiae DN127 66 kbp circular plasmid cp32-quad. Figure S10. B. finlandensis SV1 integration of cp32 into lp54. Figure S11. Borreliella and relapsing fever Borrelia PFam32 neighbor-joining tree. Table S1. Borreliella and relapsing fever Borrelia PFam32. (PDF 6030 kb

Additional file 1: Figure S1. of Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi

B. burgdorferi plasmid PFam32 protein tree. Figure S2. Comparative maps of linear plasmids in 14 B. burgdorferi isolates. Maps of the following linear plasmids are shown in the following panels: A, lp5; B, lp17; C, lp21; D, lp25; E, lp28-1; F, lp28-2, lp28-6, lp28-7 and lp28-9; G, lp28-3; H, lp28-4; I, lp28-5; J, lp28-6; K, lp32-3; L, lp36; M, lp38; N, lp54; O, lp56. Figure S3. Deletions in cp32 circular plasmids. Figure S4. Comparative cp9 plasmid maps. Figure S5. All possible recombination products. Figure S6. Ancestral transfer of genetic material from the B. burgdorferi chromosome right end to a linear plasmid. Table S1. Linear plasmid locations of selected genes. (PDF 3869 kb