Lulu regulates Shroom-induced apical constriction during neural tube closure.

Apical constriction is an essential cell behavior during neural tube closure, but its underlying mechanisms are not fully understood. Lulu, or EPB4.1l5, is a FERM domain protein that has been implicated in apical constriction and actomyosin contractility in mouse embryos and cultured cells. Interference with the function of Lulu in Xenopus embryos by a specific antisense morpholino oligonucleotide or a carboxy-terminal fragment of Lulu impaired apical constriction during neural plate hinge formation. This effect was likely due to lack of actomyosin contractility in superficial neuroectodermal cells. By contrast, overexpression of Lulu RNA in embryonic ectoderm cells triggered ectopic apico-basal elongation and apical constriction, accompanied by the apical recruitment of F-actin. Depletion of endogenous Lulu disrupted the localization and activity of Shroom3, a PDZ-containing actin-binding protein that has also been implicated in apical constriction. Furthermore, Lulu and Shroom3 RNAs cooperated in triggering ectopic apical constriction in embryonic ectoderm. Our findings reveal that Lulu is essential for Shroom3-dependent apical constriction during vertebrate neural tube closure

The carboxy-terminal fragment of Lulu functions as a dominant-negative mutant.

<p>(A) Top views of stage 9 embryos injected with indicated mRNAs. 100 pg of GFP-Lulu mRNA and 5 ng of GFP-Lulu-C or Flag-Lulu-C mRNAs were injected. Arrows point to ectopic hyper-pigmentation. (B) Percentage of the embryos showing ectopic hyper-pigmentation. Phenotypes were scored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081854#pone-0081854-g004" target="_blank">Figure 4</a> legend. (C) Lysates from embryos injected with indicated mRNAs were subjected to Western Blot. α-tubulin serves as the loading control. (D) Sections of stage 9 embryos injected with indicated mRNAs were stained with anti-GFP and anti-flag antibodies. Superficial ectodermal cells are shown. Note the change of cell shape shown by the staining of GFP. Arrows indicate constricting cells. (E) Top views of stage 17 embryos uninjected or unilaterally injected with 5 ng of Lulu-C mRNA. Asterisks mark the injected side. Arrowheads point to the hinge of neural plate, and brackets indicate disappearance of the hinge. (F) Transverse sections of the neural plate of stage 17 embryos, unilaterally injected with GFP-Lulu-C mRNAs, were stained to visualize GFP, β-catenin, and F-actin. Dashed lines mark midline. Scale bar: 20 μm. </p

Depletion of Lulu disrupts apical constriction in neuroectoderm.

<p>(A, C-D) Transverse sections of the neural plate of stage 17 embryos unilaterally injected with 250 pg of GFP-CAAX mRNA and indicated MOs were stained to visualize GFP and (A) β-catenin, (C) F-actin by phalloidin, (D) phosphorylated myosin light chain (pMLC). Representative images are shown. In (C) and (D), exposure was adjusted to avoid oversaturation of apical staining. Arrows mark the injected side. Dashed lines mark midline. Scale bar: 20 µm. (B) Ratios of apical width to apico-basal cell length of superficial neuroepithelial cells were measured and compared. Up to five cells adjacent to midline were measured per section. Means +/- s. d. are shown. ***: p<0.0001, as compared to the LuluMO group. </p

Lulu is required for neural plate hinge point formation.

<p>(A) A four-cell stage embryo viewing from the animal pole. Injections were done in one dorsal animal blastomere (arrowhead). (B) Top views of stage 18 embryos unilaterally injected with indicated morpholinos (MOs, 20 ng) and GFP-Lulu mRNA (25 pg). Asterisks mark the injected side. Squared areas are magnified on the right of each panel. Arrowheads point to the hinge, visible as a pigment line, and brackets indicate weakening or disappearance of the hinge. For quantification of defects, lack of the pigment line was scored as “severe”, and weak or discontinuous pigment line was scored as “mild.” (C) Frequencies of defects shown in (B). (D) Lysates from embryos injected with indicated mRNAs and MOs were subjected to western blot. Ponceau-S staining shows loading.</p

The FERM and FA domains of Lulu are necessary for the induction of apical constriction.

<p>(A) Schematic illustration of various deletion mutants of Lulu. Amino acid numbers are indicated. (B) Embryos were injected with 250 pg of mRNAs encoding indicated Lulu mutants. Percentage of the embryos showing ectopic hyper-pigmentation at stage 9 was shown. Phenotypes were scored based on the degree of pigmentation increase, and representative images are shown on the right side. (C) Top views of stage 9 embryos injected with indicated mRNAs. (D) Sections of stage 9 embryos injected with indicated mRNAs were stained with anti-GFP and anti-β-catenin antibodies. Superficial ectodermal cells are shown. Note the change of cell shape shown by the staining of β-catenin. Scale bar: 20 μm.</p