1 research outputs found
Farinin: Characterization of a Novel Wheat Endosperm Protein Belonging to the Prolamin Superfamily
Starch granule surface-associated
proteins were separated by HPLC
and identified by direct protein sequencing. Among the proteins identified
was one that consisted of two polypeptide chains of 11 and 19 kDa
linked by disulfide bonds. Sequencing of tryptic peptides from each
of the polypeptides revealed similarities between some of the peptides
and avenin-like b proteins encoded by partial cDNAs in NCBI. To identify
a contiguous sequence that matched all of the peptides, contigs encoding
three avenin-like b proteins were constructed from ESTs of the cultivar
Butte 86. All peptide sequences were found in a protein encoded by
one of these contigs that had not been identified previously. Protein
and DNA sequences indicated that the two polypeptide chains were derived
from a parent protein that had been cleaved at the C-terminal position
of an asparagine residue. The name farinin is suggested for this protein
and other avenin-like b proteins. Evolutionary relationships of the
protein are discussed and a simple computer molecular model was constructed.
On the basis of its sequence, the new protein was likely to be allergenic
but unlikely to be active in celiac disease