Composición en ácidos grasos de fosfolípidos y triacilgliceroles de la carne del salmonete gris de labios gruesos (Chelon labrosus) que vive en agua geotérmica y agua de mar tunecina: un estudio comparativo

This study was conducted to elucidate the effects of rearing conditions on the composition of different phospholipid (PLs) classes and triacylglycerols (TAG) of the thick-lipped grey mullet (Chelon labrosus), a muscle originating from seawater and geothermal water. The major fatty acids in the examined lipid classes of the two fish groups were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1n-9), linoleic acid (C18:2n-6), arachidonic acid (C20:4n-6), eicosapentaenoic acid (C20:5n-3), and docosahexaenoic acid (C22:6n-3). The analyses demonstrated that the fatty acid profiles of the PL classes in the seawater fish group were characterized by the predominance of n-3 polyunsaturated fatty acids (PUFA). By contrast, in geothermal fish, the distribution of PUFA series proportions differed between the phospholipid fractions. It was found PUFA n-3 was particularly abundant in PS and PI, while the n-6 series dominated the PC and PE PUFA group. Nonetheless, it was found that neutral lipid fatty acids were characterized by saturated fatty acids (SFA) followed by monounsaturated fatty acids (MUFA) in the seawater fish and by PUFA in the geothermal fish. The results presented here give useful information on the role of lipid classes in the physiological adaptation of C. labrosus which can serve for the optiminzation of these aquaculture systems.Este estudio se llevó a cabo para dilucidar los efectos de las condiciones de cría sobre la composición de diferentes clases de fosfolípidos (PL) y triacilgliceroles (TAG) del músculo de salmonetes de labios gruesos (Chelon labrosus) procedentes de agua de mar y de agua geotérmica. Los principales ácidos grasos en las clases de lípidos examinados de los dos grupos de peces fueron, palmítico (C16:0), esteárico (C18:0), oleico (C18:1n-9), linoleico (C18:2n-6), araquidónico (C20:4n-6), eicosapentaenoico (C20:5n-3) y ácido docosahexaenoico (C22:6n-3). Las determinaciones mostraron que los perfiles de ácidos grasos de los PL, en el grupo de peces de agua de mar, se caracterizaron por el predominio de ácidos grasos poliinsaturados n-3 (PUFA). Por el contrario, en los peces geotérmicos, la distribución de las proporciones de las series de PUFA difirió entre las fracciones de fosfolípidos. Se encontró que los PUFA n-3 eran particularmente abundantes en PS y PI, mientras que la serie n-6 dominaba el grupo de PUFA PC y PE. No obstante, se encontró que en lipidos neutros, los mayoritarios son los ácidos grasos saturados (SFA) seguidos de los ácidos grasos monoinsaturados (MUFA) en el pescado de agua de mar y los PUFA en el pescado geotérmico. Los resultados actuales brindan información útil sobre el papel de las clases de lípidos en la adaptación fisiológica de C. labrosus que puede servir para la optimización de estos sistemas de acuicultura

Simulation of the Periodically Perturbed Separated and Reattaching Flow over a Backward-Facing Step

In this work, the Large Eddy Simulation (LES) methodology is used to study the effects of a periodic perturbation introduced into a separated shear layer behind a backward-facing step. This study carried out by acting on the two parameters characteristics of the perturbation: frequency and amplitude. The obtained results reveal the existence of an optimum perturbation frequency value, Stp = 0.25, in terms of the reduced reattachment length. At this perturbation frequency value, we observed an increase in the vortical shedding frequency in the reattachment zone with a significant change of the structure of the flow. The value of the optimum frequency appears to be independent of the perturbation amplitude. At this frequency the maximum decrease of reattachment length is 50% and the maximum increase of vortical shedding frequency is 43 % compared to the unperturbed case

Elaboration de dépôts de TiO2 par projection plasma pour le traitement de l'eau par photocatalyse

International audienc

ANALYSIS OF LIPID CLASSES AND FATTY ACID COMPOSITION IN MUSCLE OF THE GREY MULLET CHELON LABROSUS LIVING IN TUNISIAN GEOTHERMAL WATER

Abstract Lipid classes and fatty acid composition of polar, neutral lipid fractions and the fatty acid composition of grey mullet Chelon labrosus from the geothermal water were determined. (PC) and (PE) was the main component in the Polar class as the TAG in the neutral lipid. Docosahexaenoic acid (22:6n-3) and eicosapentanoic acid (20:5n-3) were rich in the PC and PE fractions. Saturated fatty acids (SFA) were the most representative, followed by Monounsaturated fatty acids (MUFA) in the neutral fraction. The major constituents of total fatty acids in Chelon labrosus were saturated: palmitic (16:0) andstearic acid (18:0), monoenes: oleic (18:1) and palmitoleic acid (16:1), polyunsaturated: arachidonic (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic acid (22:6n-3), but their amounts differed significantly

Reproductive and tissue plasticity in Arca noae (Bivalvia: Arcidae)

The reproductive strategy of an unexploited population of Arca noae from the salt-water Bizerte Lagoon (Tunisia, western Mediterranean), including its tissue plasticity, was studied. In total 309 individuals, collected monthly from October 2013 to September 2014, were examined; 142 were females, 42 were males and five were hermaphrodites. They were used in histological and immunohistochemical (stem marker: Pou5f1; proliferation marker: proliferating cell nuclear antigen (PCNA)) analyses of gonads and adjacent tissues (N = 189) as well as to compute the monthly condition index (N = 120). Water salinity, temperature and chlorophyll a concentration were recorded. Ripe ovaries were observed in two discrete periods, Octoberâ\u80\u93November and Aprilâ\u80\u93August. Both gonad ripenings were followed by spawning periods, Novemberâ\u80\u93April and Julyâ\u80\u93September. The mature oocyte density showed that the first spawning period was less vigorous than the second one. These data also indicated that A. noae is a multiple spawner. Five cases of protandric hermaphroditism occurred from December to April. Gonad tissue was strictly associated and intermingled with the digestive gland and mantle muscle fibres. Seasonal variations were observed in the relative proportions of digestive gland and gonads: the former predominated when the latter regressed (March) and vice versa (peak in June). Seasonal transitions from germinal to somatic tissue and vice versa were hypothesised to occur through transdifferentiation mechanisms based on the activity of stem and proliferating cells. The condition index roughly increased along with gonad ripening and decreased during the spawning periods, although it did not run parallel to gonad evolution, because it also depended on chlorophyll a concentration, a proxy for phytoplankton density. The condition index was significantly correlated, by multiple regression, to both mature oocyte density and chlorophyll a concentration. Arca noae appears to have evolved a flexible reproductive strategy that makes it capable of exploiting diverse environmental conditions, which also involves tissue transdifferentiation