Effect of cold shock on adult mortality.

<p>Mortality of adults subjected to cold shock was significantly higher than the mortality of adults not subjected to cold shock. However, none of the differences between FCB (open bars) and FSB (closed bars) populations were significant.</p

Effect of cold shock on adult mortality (Experiment 1).

<p>Summary of results from a four-factor mixed model ANOVA on adult mortality data using Selection (FCB and FSB), Treatment (Both-shocked and Neither-shocked) and Sex (male and female) as fixed factors crossed with random Blocks (1–5).</p><p>Effect of cold shock on adult mortality (Experiment 1).</p

Effect of cold shock on number of mating pairs observed (Experiment 1).

<p>Summary of results from a three-factor mixed model ANOVA on the number of mating pairs observed using Selection (FCB and FSB) and Treatment (Both-shocked and Neither-shocked) as fixed factors crossed with random Blocks (1–5).</p><p>Effect of cold shock on number of mating pairs observed (Experiment 1).</p

Singh et al data

Contains data from four experiments reported in the paper

Effect of cold shock on fecundity (Experiment 1).

<p>Summary of results from a four-factor mixed model ANOVA on fecundity (eggs per female) data using Selection (FCB and FSB), Treatment (Both-shocked, Female-shocked and Neither-shocked) and Period (0 and 24 hours post cold shock) as fixed factors crossed with random Blocks (1–5).</p><p>Effect of cold shock on fecundity (Experiment 1).</p

Egg viability and fecundity post cold shock (Experiment 3).

<p>Summary of results from three-factor mixed model ANOVA on (A) Egg viability and (B) Fecundity (number of eggs per female). In this experiment, females were held without access to males post cold shock. We modeled Selection (FCB and FSB) and Treatment (Cold shock and Control) as fixed factors crossed with random Blocks (1–5).</p><p>Egg viability and fecundity post cold shock (Experiment 3).</p

Effect of cold shock on fecundity.

<p>We assayed fecundity 0 and 24 hours post cold shock. Open bars represent FCB and black bars represent FSB populations. At 0 hours after cold shock, fecundity of females subjected to cold shock (Both-shocked and Female-shocked treatments) was high compared to that of females not subjected to cold shock (Neither-shocked treatment). However, there was no such difference 24 hours after cold shock. None of the differences between FCB and FSB populations were significant.</p

Effect of cold shock on egg viability (Experiment 1).

<p>Summary of results from a four-factor mixed model ANOVA on egg viability data using Selection (FCB and FSB), Treatment (Both-shocked, Female-shocked and Neither-shocked) and Period (0 and 24 hours post cold shock) as fixed factors crossed with random Blocks (1–5).</p><p>SS: Numerator sum of squares, MS Num: Numerator mean square, DF Num: Numerator degrees of freedom, DF Den: Denominator degrees of freedom.</p><p>Effect of cold shock on egg viability (Experiment 1).</p

Effect of cold shock on larval survivorship (Experiment 2).

<p>Summary of results from a three-factor mixed model ANOVA on larval survivorship data using Selection (FCB and FSB) and Treatment (Cold shock and Control) as fixed factors crossed with random Blocks (1–5).</p><p>Effect of cold shock on larval survivorship (Experiment 2).</p