Differential growth of wrinkled biofilms

Biofilms are antibiotic-resistant bacterial aggregates that grow on moist surfaces and can trigger hospital-acquired infections. They provide a classical example in biology where the dynamics of cellular communities may be observed and studied. Gene expression regulates cell division and differentiation, which affect the biofilm architecture. Mechanical and chemical processes shape the resulting structure. We gain insight into the interplay between cellular and mechanical processes during biofilm development on air-agar interfaces by means of a hybrid model. Cellular behavior is governed by stochastic rules informed by a cascade of concentration fields for nutrients, waste and autoinducers. Cellular differentiation and death alter the structure and the mechanical properties of the biofilm, which is deformed according to Foppl-Von Karman equations informed by cellular processes and the interaction with the substratum. Stiffness gradients due to growth and swelling produce wrinkle branching. We are able to reproduce wrinkled structures often formed by biofilms on air-agar interfaces, as well as spatial distributions of differentiated cells commonly observed with B. subtilis.Comment: 19 pages, 13 figure

A microfluidic device for the study of the orientational dynamics of microrods

We describe a microfluidic device for studying the orientational dynamics of microrods. The device enables us to experimentally investigate the tumbling of microrods immersed in the shear flow in a microfluidic channel with a depth of 400 mu and a width of 2.5 mm. The orientational dynamics was recorded using a 20 X microscopic objective and a CCD camera. The microrods were produced by shearing microdroplets of photocurable epoxy resin. We show different examples of empirically observed tumbling. On the one hand we find that short stretches of the experimentally determined time series are well described by fits to solutions of Jeffery's approximate equation of motion [Jeffery, Proc. R. Soc. London. 102 (1922), 161-179]. On the other hand we find that the empirically observed trajectories drift between different solutions of Jeffery's equation. We discuss possible causes of this orbit drift.Comment: 11 pages, 8 figure

Aperiodic tumbling of microrods advected in a microchannel flow

We report on an experimental investigation of the tumbling of microrods in the shear flow of a microchannel (40 x 2.5 x 0.4 mm). The rods are 20 to 30 microns long and their diameters are of the order of 1 micron. Images of the centre-of-mass motion and the orientational dynamics of the rods are recorded using a microscope equipped with a CCD camera. A motorised microscope stage is used to track individual rods as they move along the channel. Automated image analysis determines the position and orientation of a tracked rods in each video frame. We find different behaviours, depending on the particle shape, its initial position, and orientation. First, we observe periodic as well as aperiodic tumbling. Second, the data show that different tumbling trajectories exhibit different sensitivities to external perturbations. These observations can be explained by slight asymmetries of the rods. Third we observe that after some time, initially periodic trajectories lose their phase. We attribute this to drift of the centre of mass of the rod from one to another stream line of the channel flow.Comment: 14 pages, 8 figures, as accepted for publicatio

Reservoirs and radiocarbon: ¹⁴C dating problems in Myvatnssveit, Northern Iceland

This paper examines 2 potential sources of the radiocarbon offset between human and terrestrial mammal (horse) bones recovered from Norse (~AD 870–1000) pagan graves in Mývatnssveit, north Iceland. These are the marine and freshwater 14C reservoir effects that may be incorporated into human bones from dietary sources. The size of the marine 14C reservoir effect (MRE) during the Norse period was investigated by measurement of multiple paired samples (terrestrial mammal and marine mollusk shell) at 2 archaeological sites in Mývatnssveit and 1 site on the north Icelandic coast. These produced 3 new ΔR values for the north coast of Iceland, indicating a ΔR of 106 ± 10 14C yr at AD 868–985, and of 144 ± 28 14C yr at AD 1280–1400. These values are statistically comparable and give an overall weighted mean ΔR of 111 ± 10 14C yr. The freshwater reservoir effect was similarly quantified using freshwater fish bones from a site in Mývatnssveit. These show an offset of between 1285 and 1830 14C yr, where the fish are depleted in 14C relative to the terrestrial mammals. This is attributed to the input of geothermally derived CO2 into the groundwater and subsequently into Lake Mývatn. We conclude the following: i) some of the Norse inhabitants of Mývatnssveit incorporated non-terrestrial resources into their diet that may be identified from the stable isotope composition of their bone collagen; ii) the MRE off the north Icelandic coast during the Norse period fits a spatial gradient of wider North Atlantic MRE values with increasing values to the northwest; and iii) it is important to consider the effect that geothermal activity could have on the 14C activity of samples influenced by groundwater at Icelandic archaeological sites

Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F(2α )Metabolite in Cycling Gilts and Castrated Boars

The present study was designed to evaluate the effects of synthetic ACTH (1–24, tetracosactid) and porcine CRH on the plasma levels of cortisol and PGF(2α )metabolite in cycling gilts (n = 3) and castrated boars (n = 3). The experiments were designed as crossover studies for each gender separately. Each animal received, during three consecutive days; 1) ACTH (Synacthen(® )Depot) at a dose of 10 μg/kg body weight in 5 ml physiological saline, 2) porcine CRH at a dose 0.6 μg/kg body weight in 5 ml physiological saline or 3) physiological saline (5 ml). The test substances were administered via an indwelling jugular cannula in randomized order according to a Latin square. The administration of ACTH to cycling gilts resulted in concomitant elevations of cortisol and PGF(2α )metabolite with peak levels reached at 70.0 ± 10.0 and 33.3 ± 6.7 min, respectively. Similarly, the administration of ACTH to castrated boars resulted in concomitant elevation of cortisol and PGF(2α )metabolite with peak levels reached at 60.0 ± 0.0 and 20.0 ± 0.0 min, respectively. Cortisol peaked at 20 min after administration of CRH in both cycling gilts and castrated boars with maximum levels of 149.3 ± 16.5 nmol/1 and 138.3 ± 10.1 nmol/1, respectively. It can be concluded that administration of synthetic ACTH (tetracosactid) to pigs caused a concomitant elevation of cortisol and PGF(2α )metabolite levels in both cycling gilts as well as castrated boars. The administration of CRH to pigs resulted in an elevation of cortisol levels in both cycling gilts and castrated boars. Conversely, PGF(2α )metabolite levels were not influenced by the administration of CRH either in cycling gilts or in castrated boars

Topological orders and Edge excitations in FQH states

Fractional quantum Hall (FQH) liquids contain extremely rich internal structures which represent a whole new kind of ordering. We discuss characterization and classification of the new orders (which is called topological orders). We also discuss the edge excitations in FQH liquids, which form the so-called chiral Luttinger liquids. The chiral Luttinger liquids at the edges also have very rich structures as a reflection of the rich topological orders in the bulk. Thus, edge excitations provide us a practical way to measure topological orders in experiments.Comment: 67 pages, plain-tex, 3 figures. The section about spin vector was rewritten to make it more readabl