Dactylomelane Diterpenes from the Sea Hare <i>Aplysia depilans</i>

A chemical investigation of the organic extract of the sea hare <i>Aplysia depilans</i>, collected off Skyros Island, Greece, yielded eight new brominated diterpenes (<b>1</b>–<b>8</b>), featuring the rare dactylomelane skeleton, together with the previously reported luzodiol (<b>9</b>). The structure elucidation and the assignment of the relative configurations of the new natural products were based on extensive NMR spectroscopic and MS spectrometric analyses. Compounds <b>1</b>–<b>9</b> were evaluated for their cytotoxic activities against five human tumor cell lines, but were proven inactive

Disulfides with Anti-inflammatory Activity from the Brown Alga <i>Dictyopteris membranacea</i>

Six new (<b>1</b>, <b>2</b>, and <b>4</b>–<b>7</b>) and two previously reported (<b>3</b> and <b>8</b>) disulfides, along with 4-butyl-2,6-cycloheptadienone, γ-tocopherol, and δ-tocopherol, were isolated from an organic extract of the brown alga <i>Dictyopteris membranacea</i>, collected at Gerolimenas Bay, Greece. The structure elucidation of the isolated natural products was based on analysis of their spectroscopic data. Compounds <b>1</b>, <b>3</b>–<b>6</b>, and <b>8</b> were evaluated for their antibacterial and anti-inflammatory activities. None of the compounds displayed antibacterial activity against two resistant strains of <i>Staphylococcus aureus</i> and one strain of <i>Escherichia coli</i>. In contrast, metabolite <b>5</b> was able to cause strong inhibition of NO production with an IC₅₀ value of 3.8 μM using an LPS stimulation assay

MOESM1 of Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

Additional file 1: Table S1. List of primers. Table S2. Experimental and bibliographic 1H and 13C NMR data (in CDCl3) of 11β-hydroxy-manoyl oxide (4). Figure S1. 1H NMR spectrum of 11β-hydroxy-manoyl oxide (4). Figure S2. 13C NMR spectrum of 11β-hydroxy-manoyl oxide (4)

<i>Origanum</i> species native to the island of Crete: <i>in vitro</i> antioxidant characteristics and liquid chromatography–mass spectrometry identification of major polyphenolic components

<div><p>Extracts from three <i>Origanum</i> species, including <i>Origanum microphyllum</i>, <i>Origanum dictamnus</i> and <i>Origanum vulgare</i> subsp. <i>hirtum</i>, native to the island of Crete (southern Greece), were partly fractionated through successive partition with ethyl acetate and <i>n</i>-butanol. All the fractions obtained were profiled with regard to their major polyphenolic constituents, using liquid chromatography-diode array-mass spectrometry. Furthermore, the antioxidant potency of each fraction was assessed by estimating the antiradical activity (<i>A</i>_AR) and the hydroxyl free radical scavenging activity (SA_HFR). The chromatographic analyses revealed a rich profile mainly for the ethyl acetate fractions, composed principally by flavones, which were accompanied by a limited number of phenylpropanoids, flavanones and dihydroflavonols. The highest values of antioxidant activity were displayed by the ethyl acetate extract of <i>O. dictamnus</i>, which also possessed the richest polyphenolic composition.</p></div

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10 mai 19241924/05/10 (A53).Appartient à l’ensemble documentaire : PoitouCh

Additional file 3: Figure S4. of Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

1H-NMR spectrum of pisiferic acid (compound 2). (PDF 116 kb

Additional file 9: Table S1. of Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

Thirty-five most expressed genes/contigs in M. spicata trichomes based on their FPKM values produced by the RSEM software. In the last column their top hit is referred, produced by BLAST searches in the NR database. The length of the contigs is sometimes not an integer number due to the fact that one contig may correspond to several transcripts of diverse length. The RSEM software was run on the data produced by the transcriptome sequencing of M. spicata trichomes by Jin et al. [37]. (PDF 103 kb

Additional file 4: Figure S5. of Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

1H-NMR spectrum of O-methyl-pisiferic acid (compound 3). (PDF 144 kb